Identification of differentially expressed mRNAs in human fetal liver across gestation

Malhotra, Khushbeer; Luehrsen, Kenneth R.; Costello, Lawrence; Raich, Teresa J.; Sim, Kim; Foltz, Lisa; Davidson, Scott; Xu, Hongxia; Chen, Audrey; Yamanishi, Douglas T.; Lindemann, Garrett W.; Cain, Carol A.; Madlansacay, Mary Rose; Hashima, S.; Pham, Thu Lan; Mahoney, Walt; Schueler, Paula A.

doi:10.1093/nar/27.3.839

Cited by 46 publications

(38 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This may suggest that MAC30 is one of the developmentally regulated proteins that are expressed in the hematopoietic development (2). MAC30 was first described to be overexpressed in meningiomas, and altered expression was also found in different types of human tumors (1)(2)(3). MAC30 is expressed at moderate levels in normal pancreatic acinar cells, strongly present in tubular complexes of chronic pancreatitis, and observed at low levels in most pancreatic cancer cells.…”

Section: Introductionmentioning

confidence: 99%

Expression of MAC30 protein is related to survival and biological variables in primary and metastatic colorectal cancers

Moparthi¹,

Arbman²,

Wallin³

et al. 2007

Int J Oncol

View full text Add to dashboard Cite

Abstract. MAC30 is highly expressed in several types of tumors including colorectal cancers, however, its clinicopathological and biological significance in colorectal cancers is currently not known. The aim of our study was to investigate MAC30 expression in distant normal mucosa, adjacent normal mucosa, primary tumors and metastases of colorectal cancer, and to determine the relationship between MAC30 expression and clinicopathological and biological variables. MAC30 expression was immunohistochemically examined in distant normal mucosa (n=54), adjacent normal mucosa (n=123), primary tumors (n=217) and lymph node metastases (n=56) from colorectal cancer patients. MAC30 cytoplasmic expression was increased from distant normal mucosa to primary tumor and to metastasis (p<0.0001-0.04). Furthermore, 40% primary and 37% metastatic tumors showed stronger cytoplasmic expression of MAC30 at the tumor invasive margins compared to inner tumor areas. Strong cytoplasmic expression of MAC30 in the metastasis was related to a poor prognosis (p=0.04). MAC30 cytoplasmic expression was positively related to expression of proliferating cell nuclear antigen (p=0.04), p53 (p=0.04), nucleoporin 88 (p=0.001), legumain (p=0.004) and particularly interesting new cysteine-histidine rich protein (p=0.004). However, MAC30 expression in the nucleus and stroma did not have any clinicopathological and biological significance (p>0.05). In conclusion, MAC30 protein may play a role in development of colorectal cancer, and can be considered as a prognostic factor.

show abstract

Section: Introductionmentioning

confidence: 99%

Expression of MAC30 protein is related to survival and biological variables in primary and metastatic colorectal cancers

Moparthi¹,

Arbman²,

Wallin³

et al. 2007

Int J Oncol

View full text Add to dashboard Cite

show abstract

“…14 In addition, hepatoblasts express ␣-fetoprotein (AFP), insulin-like growth factor-2 (IGF), IGF-binding proteins, and some ribosomal proteins not expressed by hepatocytes. 15 Hepatocytic maturation is marked by the expression of a number of genes, proteases (eg, cathepsin L), acute phase proteins (eg, orosomucoid), enzymes involved in the metabolism of nucleotide intermediates (eg, nicotinamide methyl-transferase), of glucids (eg, glucose-6-phosphatase), and of amino acids (eg, tyrosine-aminotransferase) characteristic of the different liver metabolic functions. 15,16 Finally, many proteins, such as cytokeratins 8 and 18 and albumin, expressed by hepatoblasts continue to be expressed by hepatocytes.…”

Section: Introductionmentioning

confidence: 99%

“…15 Hepatocytic maturation is marked by the expression of a number of genes, proteases (eg, cathepsin L), acute phase proteins (eg, orosomucoid), enzymes involved in the metabolism of nucleotide intermediates (eg, nicotinamide methyl-transferase), of glucids (eg, glucose-6-phosphatase), and of amino acids (eg, tyrosine-aminotransferase) characteristic of the different liver metabolic functions. 15,16 Finally, many proteins, such as cytokeratins 8 and 18 and albumin, expressed by hepatoblasts continue to be expressed by hepatocytes. 13,15 In many respects, hepatoblasts appear similar to oval cells proliferating in response to liver damage.…”

Section: Introductionmentioning

confidence: 99%

Fetal liver stroma consists of cells in epithelial-to-mesenchymal transition

Chagraoui

Lepage-Noll

Anjo

et al. 2003

Blood

140

100

View full text Add to dashboard Cite

Liver becomes the predominant site of hematopoiesis by 11.5 dpc (days after coitus) in the mouse and 15 gestational weeks in humans and stays so until the end of gestation. The reason the liver is the major hematopoietic site during fetal life is not clear. In this work, we tried to define which of the fetal liver microenvironmental cell populations would be associated with the development of hematopoiesis and found that a population of cells with mixed endodermal and mesodermal features corresponded to hematopoieticsupportive fetal liver stroma. Stromal cells generated from primary cultures or stro-

show abstract

“…To date several studies have reported that the imprinted Igf2 [20][21][22][23][24] and Dlk1 [18,19,25] genes are involved in the development of fetal liver hematopoiesis. During the first half of the embryonic period, the space between the hepatic cell cords Igf2 and Dlk1 expression were quantitatively analyzed by realtime PCR.…”

Section: Discussionmentioning

confidence: 99%

Synergistic Role of Igf2 and Dlk1 in Fetal Liver Development and Hematopoiesis in Bi-Maternal Mice

Kawahara

Kono

2008

Journal of Reproduction and Development

View full text Add to dashboard Cite

Abstract. Mouse bi-maternal embryos (BMEs) that contain two haploid sets of genomes from non-growing (ng) and fully-grown (fg) oocytes develop to embryonic day (E) 13.5. However, the ng/fg BMEs never develop beyond E13.5 because of repression of the paternally expressed imprinted genes, Igf2 and Dlk1. The present study was conducted to address the issue of whether fetal hematopoietic disorder is involved in the restricted development of BMEs. FACS analysis revealed that the livers of ng wt /fg BMEs contained increased numbers of immature c-kit + /ter119 -hematopoietic cells, were while the numbers of mature c-kit -/ter119 + hematopoietic cells were decreased. This finding was supported by histological observations. Quantitative gene expression analysis revealed that Igf2 and Dlk1 expression was repressed in the liver. To understand the role of paternally-methylated imprinted genes on chromosomes 7 and 12, particularly Igf2 and Dlk1, in fetal liver hematopoiesis, we constructed ng Δch7 /fg, ng [177][178][179][180][181][182] 2008) ue to imprinted genes, which exhibit monoallelic expression depending upon parental origin-dependent epigenetic modifications, such as DNA methylation imposed during gametogenesis, mouse parthenogenetic embryos never develop beyond day 10 of gestation in mice [1][2][3][4]. However, reconstructed bi-maternal embryos (ng/fg BMEs), which contain 2 haploid sets of genomes obtained from a non-growing stage oocyte (ng) and a fully grown oocyte (fg), develop to embryonic day (E) 13.5 [5]. A detailed gene expression analysis revealed that this extended development of ng/fg BMEs results from the appropriate expression of a majority of the imprinted genes: however, the paternally-methylated and paternally-expressed genes are still repressed [6,7]. To induce expression of Igf2 and Dlk1 from the ng oocyte allele, we reconstructed ng/fg BMEs using ng oocytes harboring a deletion in the H19 transcription unit and its differentially methylated region (Δch7) or a deletion in the methylated region IG-DMR (Δch12) in the ng allele. We demonstrated that both genotypes of ng Δch7 /fg BMEs [7] and ng Δch12 /fg BMEs [8] developed to E18.5, but also that they experienced severe growth retardation and died soon after recovery from the uterus. Furthermore, we recently demonstrated that ng/fg BMEs harboring both Δch7 and Δch12 (ΔDouble) expressed Igf2 and Dlk1 at appropriate levels and developed to normal adults at high frequency [9]. From these results, it has been suggested that both Igf2 and Dlk1 are critical for normal mouse embryonic development, while these paternally expressed imprinted genes function as a strict barrier to development beyond mid-gestation (E13.5) in mice [7,8,10]. However, phenotypical analyses for ng/fg bi-maternal fetuses have rarely been reported. In order to gain a further understanding of the features of the bi-maternal fetuses, we focused on hematopoiesis because the fetal hematopoietic system in the liver is critically important for survival and growth of mouse embryos. In mouse...

show abstract

Identification of differentially expressed mRNAs in human fetal liver across gestation

Cited by 46 publications

References 30 publications

Expression of MAC30 protein is related to survival and biological variables in primary and metastatic colorectal cancers

Expression of MAC30 protein is related to survival and biological variables in primary and metastatic colorectal cancers

Fetal liver stroma consists of cells in epithelial-to-mesenchymal transition

Synergistic Role of Igf2 and Dlk1 in Fetal Liver Development and Hematopoiesis in Bi-Maternal Mice

Contact Info

Product

Resources

About