Identification of differentially expressed microRNAs and the potential of microRNA‑455‑3p as a novel prognostic biomarker in glioma

Abstract

Glioma is an aggressive central nervous system malignancy. MicroRNAs (miRNAs/miRs) have been reported to be involved in the tumorigenesis of numerous types of cancer, including glioma. The present study aimed to identify the differentially expressed miRNAs in glioma, and further explore the clinical value of miR-455-3p in patients with glioma. GEO2R was used for the identification of the differentially expressed miRNAs according to the miRNA expression profiles obtained from the Gene Expression Omnibus database. OncomiR was used to analyze the relationship of miRNAs with the survival outcomes of the patients with glioma. A total of 108 patients with glioma were recruited to examine the expression levels of miR-455-3p and further explore its clinical value. The bioinformatics analysis results suggested that a total of 64 and 48 differentially expressed miRNAs were identified in the GSE90603 and GSE103229 datasets, respectively. There were 12 miRNAs in the overlap of the two datasets, of which three were able to accurately predict overall cancer survival, namely hsa-miR-7-5p, hsa-miR-21-3p and hsa-miR-455-3p. In patients with glioma, miR-455-3p was determined to be significantly upregulated (P<0.001). Additionally, patients with high miR-455-3p expression had significantly lower 5-year overall survival than those with low miR-455-3p expression (log-rank test, P=0.001). Cox regression analysis further determined that miR-455-3p was an independent prognostic indicator for overall survival in patients with glioma (hazard ratio=2.136; 95% CI=1.177-3.877; P=0.013). In conclusion, the present study revealed a series of miRNAs with potential functional roles in the pathogenesis of glioma, and provides findings that indicate miR-455-3p as a promising biomarker for the prognosis of glioma.

CeRNA Network Analysis Representing Characteristics of Different Tumor Environments Based on 1p/19q Codeletion in Oligodendrogliomas

“…In addition, in the results of an expanded study on lower grade glioma, more statistically significant genes were found. There were reports with findings consistent with those of ours on hsa-miR-1262, hsa-miR-455-3p, LPAL2 [83][84][85][86], and some reports even contradicted our findings on hsa-miR-204-5p and hsa-miR-197-3p [87,88]. Although related studies are still insufficient, the previous report about hsa-miR-186-5p in glioblastoma contradicted our findings [89].…”

A Novel miRNA–mRNA Axis Involves in Regulating Transcriptional Disorders in Pancreatic Adenocarcinoma

“… 26 However, in glioma patients, the miRNA was noted to be significantly upregulated, and an increasing expression of this miRNA was associated with decreasing five-year overall survival rates. 27 The specific mechanism of miR-455-3p, with respect to PAAD, is still unclear. Therefore, further studies on the expression and mechanism of miR-455-3p may assist in examining it as a potential early biomarker and tumor suppressor gene.…”

Regulation of gene expression by miRNA-455-3p, upregulated in the conjunctival epithelium of patients with Stevens–Johnson syndrome in the chronic stage

“…Also, miR-455 has been proposed as a cancer biomarker and it may be involved in the etiology of cancer. Wang et al 18 reported that in patients with glioma, miR-455-3p was significantly upregulated and according to Liu et al 19 , miR-455-3p elicited aberrant up-regulation in various human cancer types and was significantly associated with the lower overall survival of cancer patients. Moreover, Liu et al 20 found that miRNA455-3p promotes the invasion and migration of targeting tumor suppressor etoposide-induced 2.4 and suggested that it might be a potential prognostic biomarker and a therapeutic target in patients with triple-negative breast cancer.…”