Identification of differentially expressed long non-coding RNAs and messenger RNAs involved with muscle development in Dazu black goats through RNA sequencing

Integrated transcriptome analysis reveals roles of long non-coding RNAs (lncRNAs) in caprine skeletal muscle mass and meat quality

“…The characteristics of lncRNAs identi ed in this study are in concordance with previous studies in skeletal muscle tissue. For example, intergenic lncRNA was found to be the most common in skeletal muscle of Dazu black goats [24], rabbit [34] and cattle [13]. Our observation that known lncRNAs had shorter transcript length than mRNAs, is consistent with ndings in Anhui white goats [22], cattle [13] and donkey [35].…”

“…For example, a total of 547 lncRNAs were differentially expressed in skeletal muscles of Anhui white goats among ve fetal stages and two kid stages, and they were involved in signaling pathways closely associated with muscle development, including structure formation, p53 signaling pathway, and MAPK signaling pathway [22]. Additionally, 577 and 648 differentially expressed lncRNAs were found in skeletal muscle tissues between embryonic and postnatal stages of Jianzhou big-eared goats and Dazu black goats, respectively [23][24]. However, there have been no reports on comparison of lncRNAs expression pro les in skeletal muscle tissues between different goat breeds.…”

“…The RT-qPCR reaction was conducted in triplicate using 2 × ChamQ SYBR qPCR Master Mix (Vazyme, Nanjing, China). The expression levels of the lncRNAs were normalized by caprine GAPDH [24] and β-actin [23] and then calculated using 2 −ΔΔCt method. The primer information involved was listed in Table 1.…”

Temporal Transcriptome Dynamics of Longissimus dorsi Reveals the Mechanism of the Differences in Muscle Development and IMF Deposition between Fuqing Goats and Nubian Goats

“…In this study, the expression of genes related to myoblast fusion, skeletal muscle satellite cell proliferation, and growth factors, including MYOD1 [ 9 ], MYF5 [ 45 ], PAX7 [ 48 ], and MSTN [ 49 ], was greater in FE3 than in NE3, even though the transcript levels of several other myogenesis-related genes ( MEF2A [ 48 ] and IGF2 [ 48 ]) were similar ( Figure 15 ). Interestingly, many downregulated DEGs ( KIF23 , CCDC69 , CCNA2 , MKI67 , KIF11 , RACGAP1 , NUSAP1 , SKP2 , ZBTB18 , NES , LOC102180034 , CAPN6 , TUBA1A , LOC102178700 , and PEG10 ) from the FE3 vs. NE3 set were significantly enriched in the cell cycle (ko04110) and DNA replication (ko03030) terms ( Figure 16 ), which indicated that the differentiation and fusion of myogenesis between FQs and NBYs were not consistent and that the myogenesis of FQs progressed more slowly in FQs than in NBYs.…”