Identification of differentially expressed genes in dormant (banjhi) bud of tea (Camellia sinensis (L.) O. Kuntze) using subtractive hybridization approach

Thirugnanasambantham, Krishnaraj; Gajjeraman, Prabu; Palanisamy, S.; Chandrabose, Suresh Ramraj Subhas; Mandal, Abul Kalam Azad

doi:10.1016/j.plaphy.2011.03.011

Cited by 27 publications

(8 citation statements)

References 36 publications

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“…Except for a few exclusive GO terms attributed to some groups of unigenes from each cultivar, overall they shared the same GO terms. Other studies of bud dormancy using the SSH technique have described subtractions with transcripts belonging to overlapping functions, even between reciprocal libraries (Leida et al 2010;Krishnaraj et al 2011). This functional equivalence indicates that basal metabolic pathways are equally active throughout the dormancy cycle, although the genes involved may not be necessarily the same.…”

Section: Functional Enrichment Of Genes Identified By Sshmentioning

confidence: 99%

Differential Transcriptional Profiles of Dormancy-Related Genes in Apple Buds

Falavigna

Porto²,

Buffon³

et al. 2013

Plant Mol Biol Rep

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The production of temperate fruit crops depends on plant developmental processes, primarily the shift from the juvenile phase to the reproductive phase, dormancy transitions and flowering. Apple tree (Malus ×domestica Borkh.) development is regulated by chilling temperatures, which are required for bud dormancy progression. The apple cultivar Castel Gala is a spontaneous mutation of "Gala Standard". "Castel Gala" is characterized by a 50 % decrease in the chilling requirement (CR) for dormancy release, which results in an earlier budbreak. This work explores the contrasting phenotypes of these cultivars using suppression subtractive hybridization (SSH). From 1,019 unigenes identified by SSH, we selected 28 candidate genes putatively associated with dormancy cycling. Reverse transcription-quantitative polymerase chain reaction was used to validate the differential expression profiles and to transcriptionally characterize these genes in three distinct apple cultivars ("Castel Gala", "Royal Gala" and "Fuji Standard") during a cycle comprising growth to dormancy. Of the 28 candidate genes analyzed, 17 confirmed the differences in expression predicted by SSH. Seasonal transcript accumulation during the winter was observed for several genes, with higher steady-state mRNA levels maintained longer in cultivars with a high CR. The transcription profiles suggest that these genes may be associated with dormancy establishment and maintenance. Of the 17 candidate genes, transcripts coding for dormancy-associated MADS-box (DAM), dehydrins, GAST1, LTI65, NAC, HTA8, HTA12 and RAP2.12-like proteins displayed major differences in gene expression between cultivars through the winter. These genes were therefore considered good candidates for key roles in the dormancy process in apple trees. Keywords Apple . Bud dormancy . Gene expression . Malus ×domestica . RT-qPCR . Suppression subtractive hybridization Abbreviations ABA Abscisic acid AP2 APETALA2 ARC5 Accumulation and replication of chloroplast 5 ARP6 Actin-related protein 6 CAMTA1 Calmodulin-binding transcription activator 1 CBF C-repeat binding factor CO CONSTANS COR Cold-regulated CR Chilling requirement CRT C-repeat DAM Dormancy-associated MADS-box DHN Dehydrin DRE Dehydration-responsive element DREB Dehydration-responsive element binding protein EST Expressed sequence tag FT FLOWERING LOCUS T GAST1 GA stimulated transcript 1 GO Gene ontologyThe nucleotide sequences reported in this paper have been submitted to GenBank with the accession numbers JZ480898 to JZ482228.

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Section: Functional Enrichment Of Genes Identified By Sshmentioning

confidence: 99%

Differential Transcriptional Profiles of Dormancy-Related Genes in Apple Buds

Falavigna

Porto²,

Buffon³

et al. 2013

Plant Mol Biol Rep

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“…It is a momentous economic crop in the world and has terrific value as sources of tea polyphenols, caffeine, theanine and vitamin etc. (Krishnaraj et al, 2011;Thirugnanasambantham et al, 2013). Tea plant is thermophilic and poor in cold tolerance during winter.…”

Section: Introductionmentioning

confidence: 99%

Metabolite profiling of tea (Camellia sinensis L.) leaves in winter.

Shen

Wang

Chen

et al. 2015

Scientia Horticulturae

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“…However, these authors have carried out a seasonal monitoring and no cold/dark treatment was applied. The successfully isolated partial gene CjERF was highly similar to OsEREBP1 (rice), a transcriptional activator involved in defense signaling pathway ( Cheong et al, 2003 ), belonging to the AP2/ERF family transcription factors that is generally induced by cold as seen in Arabidopsis ( Thomashow, 2010 ), rice ( Tian et al, 2011 ), and C. sinensis ( Krishnaraj et al, 2011 ). Their main role is to bind to cis -elements in the promoters of cor (cold-regulated) genes and activate their expression ( Chinnusamy et al, 2007 ).…”

Section: Discussionmentioning

confidence: 99%

“…Dormancy leads also to changes in the expression of a large pool of genes ( Horvath et al, 2003 ; Horvath, 2009 ). As emerged from recent studies concerning cold-induced and winter dormant C. sinensis (L.) Kuntze ( Wang et al, 2009 , 2014 ; Krishnaraj et al, 2011 ; Paul and Kumar, 2011 , 2013 ), several genes responsible for the primary metabolism and stress responsive pathways are involved in the initiation, maintenance and release of dormancy, and can function as dormancy breaking markers. More in detail, some of them are commonly recognized as cold-regulated ( cor ) genes involved in cold acclimation (CAP Arabidopsis cor genes; Breton et al, 2003 ) or are known to activate cor genes themselves (AP2/ERF family transcription factor genes; Chinnusamy et al, 2007 ).…”

Section: Introductionmentioning

confidence: 99%

Cold Treatment Breaks Dormancy but Jeopardizes Flower Quality in Camellia japonica L.

Berruti

Christiaens

Keyser³

et al. 2015

Front. Plant Sci.

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Camellia japonica L. is an evergreen shrub whose cultivars are of great ornamental value. In autumn, after flower bud differentiation, dormancy is initiated. As in many other spring flowering woody ornamentals, winter low temperatures promote dormancy release of both flower and vegetative buds. However, warm spells during late autumn and winter can lead to unfulfilled chilling requirements leading to erratic and delayed flowering. We hypothesized that storing plants at no light and low temperature could favor dormancy breaking and lead to early and synchronized flowering in response to forcing conditions in C. japonica ‘Nuccio’s Pearl’. Plants with fully developed floral primordia were stored at dark, 7°C, and RH > 90% for up to 8 weeks. To monitor endodormancy release during the storage, we evaluated the content of abscisic acid (ABA) in flower buds and the expression profiles of five putative genes related to dormancy and cold acclimation metabolism in leaves and flower buds. In addition, the expression of four anthocyanin biosynthesis pathway genes was profiled in flower buds to assess the effect of the treatment on flower pigment biosynthesis. At 0, 4, 6, and 8 weeks of cold treatment, 10 plants were transferred to the greenhouse and forced to flower. Forced plant flower qualities and growth were observed. The ABA content and the expression profiles of two dormancy-related genes (CjARP and CjDEH) suggested that dormancy breaking occurred after 6–8 weeks of cold treatment. Overall, plants treated for 6–8 weeks showed earlier vegetative sprouting, enhanced, and homogeneous flowering with reduced forcing time. Prolonged cold treatments also reduced flower size and longevity, anthocyanin content, and pigment biosynthesis-related gene transcripts. In conclusion, the cold treatment had a promotive effect on dormancy breaking but caused severe drawbacks on flower quality.

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Identification of differentially expressed genes in dormant (banjhi) bud of tea (Camellia sinensis (L.) O. Kuntze) using subtractive hybridization approach

Cited by 27 publications

References 36 publications

Differential Transcriptional Profiles of Dormancy-Related Genes in Apple Buds

Differential Transcriptional Profiles of Dormancy-Related Genes in Apple Buds

Metabolite profiling of tea (Camellia sinensis L.) leaves in winter.

Cold Treatment Breaks Dormancy but Jeopardizes Flower Quality in Camellia japonica L.

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