Identification of differentially expressed genes of Xanthomonas axonopodis pv. citri by representational difference analysis of cDNA

Mehta, Ângela; Rosato, Yoko Bomura

doi:10.1590/s1415-47572005000100024

Cited by 7 publications

(6 citation statements)

References 44 publications

(57 reference statements)

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“…The 50S ribosomal proteins L25, L5 and L18 are 5S RNA binding proteins, and it has been reported that the presence of L25 is essential for the stimulation of L5 binding (Newberry & Garrett, 1980). The differential expression of ribosomal proteins was reported by Mehta & Rosato (2005) when analysing the gene expression of X. axonopodis pv. citri in different culture conditions.…”

Section: Resultsmentioning

confidence: 99%

In vivo proteome analysis of Xanthomonas campestris pv. campestris in the interaction with the host plant Brassica oleracea

Andrade

Silva

Pereira³

et al. 2008

FEMS Microbiology Letters

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The genus Xanthomonas is composed of several species that cause severe crop losses around the world. In Latin America, one of the most relevant species is Xanthomonas campestris pv. campestris, which is responsible for black rot in cruciferous plants. This pathogen causes yield losses in several cultures, including cabbage, cauliflower and broccoli. Although the complete structural genome of X. campestris pv. campestris has been elucidated, little is known about the protein expression of this pathogen in close interaction with the host plant. Recently, a method for in vivo analysis of Xanthomonas axonopodis pv. citri was developed. In the present study, this technique was employed for the characterization of the protein expression of X. campestris pv. campestris in close interaction with the host plant Brassica oleracea. The bacterium was infiltrated into leaves of the susceptible cultivar and later recovered for proteome analysis. Recovered cells were used for protein extraction and separated by two-dimensional electrophoresis. Proteins were analysed by peptide mass fingerprinting or de novo sequencing and identified by searches in public databases. The approach used in this study may be extremely useful in further analyses in order to develop novel strategies to control this important plant pathogen.

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Section: Resultsmentioning

confidence: 99%

In vivo proteome analysis of Xanthomonas campestris pv. campestris in the interaction with the host plant Brassica oleracea

Andrade

Silva

Pereira³

et al. 2008

FEMS Microbiology Letters

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“…Although the Xoo MAI1 SSH-microarray does not allow analyses of genome-wide gene expression profiles, specific biological questions can be answered more efficiently, for example, identification of virulence determinants in African Xoo strains. In contrast with other large-scale approaches to the study of gene expression in the Xanthomonas genus [ 10 , 16 , 25 , 29 , 30 ], this is the first report to compare bacterial gene expression in planta and at different time points during infection.…”

Section: Introductionmentioning

confidence: 98%

In planta gene expression analysis of Xanthomonas oryzae pathovar oryzae, African strain MAI1

et al. 2010

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BackgroundBacterial leaf blight causes significant yield losses in rice crops throughout Asia and Africa. Although both the Asian and African strains of the pathogen, Xanthomonas oryzae pv. oryzae (Xoo), induce similar symptoms, they are nevertheless genetically different, with the African strains being more closely related to the Asian X. oryzae pv. oryzicola (Xoc).ResultsChanges in gene expression of the African Xoo strain MAI1 in the susceptible rice cultivar Nipponbare were profiled, using an SSH Xoo DNA microarray. Microarray hybridization was performed comparing bacteria recovered from plant tissues at 1, 3, and 6 days after inoculation (dai) with bacteria grown in vitro. A total of 710 bacterial genes were found to be differentially expressed, with 407 up-regulated and 303 down-regulated. Expression profiling indicated that less than 20% of the 710 bacterial transcripts were induced in the first 24 h after inoculation, whereas 63% were differentially expressed at 6 dai. The 710 differentially expressed genes were one-end sequenced. 535 sequences were obtained from which 147 non-redundant sequences were identified. Differentially expressed genes were related to metabolism, secretion and transport, pathogen adherence to plant tissues, plant cell-wall degradation, IS elements, and virulence. In addition, various other genes encoding proteins with unknown function or showing no similarity to other proteins were also induced. The Xoo MAI1 non-redundant set of sequences was compared against several X. oryzae genomes, revealing a specific group of genes that was present only in MAI1. Numerous IS elements were also found to be differentially expressed. Quantitative real-time PCR confirmed 86% of the identified profile on a set of 14 genes selected according to the microarray analysis.ConclusionsThis is the first report to compare the expression of Xoo genes in planta across different time points during infection. This work shows that as-yet-unidentified and potentially new virulence factors are appearing in an emerging African pathogen. It also confirms that African Xoo strains do differ from their Asian counterparts, even at the transcriptional level.

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“…Xac recovery was performed as described by Mehta and Rosato [ 132 ], with some modifications. The citrus leaves were excised from plants at 3 or 5DAI.…”

Section: Methodsmentioning

confidence: 99%

“…The extraction of total proteins was performed as described by Mehta and Rosato [ 132 ], with some modifications. Each Xac pellet was washed in phosphate buffer (1.24 g/L K 2 HPO 4 ; 0.39 g/L KH 2 PO 4 ; 8.8 g/L NaCl, pH 7.2).…”

Section: Methodsmentioning

confidence: 99%

Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp. citri during Citrus sinensis infection

et al. 2017

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Background Xanthomonas citri subsp. citri (Xac) is the causal agent of citrus canker. A proteomic analysis under in planta infectious and non-infectious conditions was conducted in order to increase our knowledge about the adaptive process of Xac during infection.ResultsFor that, a 2D–based proteomic analysis of Xac at 1, 3 and 5 days after inoculation, in comparison to Xac growth in NB media was carried out and followed by MALDI-TOF-TOF identification of 124 unique differentially abundant proteins. Among them, 79 correspond to up-regulated proteins in at least one of the three stages of infection. Our results indicate an important role of proteins related to biofilm synthesis, lipopolysaccharides biosynthesis, and iron uptake and metabolism as possible modulators of plant innate immunity, and revealed an intricate network of proteins involved in reactive oxygen species adaptation during Plants` Oxidative Burst response. We also identified proteins previously unknown to be involved in Xac-Citrus interaction, including the hypothetical protein XAC3981. A mutant strain for this gene has proved to be non-pathogenic in respect to classical symptoms of citrus canker induced in compatible plants.ConclusionsThis is the first time that a protein repertoire is shown to be active and working in an integrated manner during the infection process in a compatible host, pointing to an elaborate mechanism for adaptation of Xac once inside the plant.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-017-1063-x) contains supplementary material, which is available to authorized users.

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Identification of differentially expressed genes of Xanthomonas axonopodis pv. citri by representational difference analysis of cDNA

Cited by 7 publications

References 44 publications

In vivo proteome analysis of Xanthomonas campestris pv. campestris in the interaction with the host plant Brassica oleracea

In vivo proteome analysis of Xanthomonas campestris pv. campestris in the interaction with the host plant Brassica oleracea

In planta gene expression analysis of Xanthomonas oryzae pathovar oryzae, African strain MAI1

Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp. citri during Citrus sinensis infection

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