Identification of differentially expressed genes of primary spermatocyte against round spermatid isolated from human testis using the laser capture microdissection technique

Liang, Gang; Zhang, Xiao Dong; Wang, Lu Jing; Sha, Yu Shen; Zhang, Jian Chao; Miao, Shiying; Zong, Shu Dong; Wang, Lin Fang; Koide, S. S.

doi:10.1038/sj.cr.7290254

Cited by 42 publications

(24 citation statements)

References 21 publications

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“…It has been postulated that it may be involved in functions other than electron transfer in complex IV, such as gamete maturation, fertilization, and/or embryogenesis (Chakrabarti et al, 2006(Chakrabarti et al, , 2007(Chakrabarti et al, , 2009Chapman et al, 2008). These speculations are substantiated by earlier reports of enhanced expression of cox2 during rat and human spermatogenesis (Saunders et al, 1993;Liang et al, 2004).…”

Section: Introductionsupporting

confidence: 72%

Doubly uniparental inheritance and highly divergent mitochondrial genomes of the freshwater mussel Unio tumidus (Bivalvia: Unionidae)

Soroкa

Burzyński

2017

Hydrobiologia

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Unio tumidus is a native European freshwater mussel from the family Unionidae. These mussels have a unique system of mitochondrial DNA inheritance called doubly uniparental inheritance (DUI). Under DUI, two types of mitochondrial DNA are present: haplotype F (female genome)-inherited from mother and haplotype M (male genome)-inherited from fathers to male offspring. The F genome occurs in eggs and in somatic tissues of both sexes, whereas the M genome is present in male gonads and gametes. We characterized three M and three F mitochondrial genomes of Unio tumidus. The lengths of these genomes were 15769-15770 bp for F type and 16607 bp for M type. In both genomes, the set of 38 genes which is typical for Unionidae was identified: 37 metazoan genes and gender-specific ORFs. The non-coding sequences constituted only 5.2 and 3.5% of F and M genome, respectively. Both genomes were similarly high in average AT content (65-66%) but intraspecific nucleotide diversity amongst the three M genomes of U. tumidus was four times lower than amongst F genomes. The patterns of polymorphisms across the mitogenomes of the closest relatives confirmed that the M genomes accumulate more substitutions and the conserved regions within one lineage are also conserved in the other.

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Section: Introductionsupporting

confidence: 72%

Doubly uniparental inheritance and highly divergent mitochondrial genomes of the freshwater mussel Unio tumidus (Bivalvia: Unionidae)

Soroкa

Burzyński

2017

Hydrobiologia

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“…Its presence may reflect the energy requirements needed during meiosis (Saunders et al, 1993). It is also present in pachytene spermatocytes of human testis (Liang et al, 2004).…”

Section: Mitochondriamentioning

confidence: 99%

Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 4: Intercellular bridges, mitochondria, nuclear envelope, apoptosis, ubiquitination, membrane/voltage‐gated channels, methylation/acetylation, and transcription factors

Hermo

Pelletier

Cyr

et al. 2009

Microscopy Res & Technique

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As germ cells divide and differentiate from spermatogonia to spermatozoa, they share a number of structural and functional features that are common to all generations of germ cells and these features are discussed herein. Germ cells are linked to one another by large intercellular bridges which serve to move molecules and even large organelles from the cytoplasm of one cell to another. Mitochondria take on different shapes and features and topographical arrangements to accommodate their specific needs during spermatogenesis. The nuclear envelope and pore complex also undergo extensive modifications concomitant with the development of germ cell generations. Apoptosis is an event that is normally triggered by germ cells and involves many proteins. It occurs to limit the germ cell pool and acts as a quality control mechanism. The ubiquitin pathway comprises enzymes that ubiquitinate as well as deubiquitinate target proteins and this pathway is present and functional in germ cells. Germ cells express many proteins involved in water balance and pH control as well as voltage-gated ion channel movement. In the nucleus, proteins undergo epigenetic modifications which include methylation, acetylation, and phosphorylation, with each of these modifications signaling changes in chromatin structure. Germ cells contain specialized transcription complexes that coordinate the differentiation program of spermatogenesis, and there are many male germ cell-specific differences in the components of this machinery. All of the above features of germ cells will be discussed along with the specific proteins/genes and abnormalities to fertility related to each topic.

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“…27,28 Alternatively, stage-specific meiotic cells can be obtained by microdissection and can also be used as a source of RNA. 29,30 However, both of these techniques are technically demanding and time consuming. To simplify the strategy, total testis samples obtained during progression of the first round of spermatogenesis can also be applied for this purpose.…”

Section: Screening Of Genes Involved In Meiosis I H Kogo Et Almentioning

confidence: 99%

Screening of genes involved in chromosome segregation during meiosis I: toward the identification of genes responsible for infertility in humans

et al. 2010

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Prophase I of male meiosis during early spermatogenesis involves dynamic chromosome segregation processes, including synapsis, meiotic recombination and cohesion. Genetic defects in the genes that participate in these processes consistently cause reproduction failure in mice. To identify candidate genes responsible for infertility in humans, we performed gene expression profiling of mouse spermatogenic cells undergoing meiotic prophase I. Cell fractions enriched in spermatogonia, leptotene/zygotene spermatocytes or pachytene spermatocytes from developing mouse testis were separately isolated by density gradient sedimentation and subjected to microarray analysis. A total of 726 genes were identified that were upregulated in leptotene/zygotene spermatocytes. To evaluate the screening efficiency for meiosis-specific genes, we randomly selected 12 genes from this gene set and characterized each gene product using reverse transcription (RT)-PCR of RNA from gonadal tissues, in situ hybridization on testicular tissue sections and subcellular localization analysis of the encoded protein. Four of the 12 genes were confirmed as genes expressed in meiotic stage and 2 of these 4 genes were novel, previously uncharacterized genes. Among the three confirmation methods that were used, RT-PCR appeared to be the most efficient method for further screening. These 726 candidates for human infertility genes might serve as a useful resource for next-generation sequencing combined with exon capture by microarray.

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Identification of differentially expressed genes of primary spermatocyte against round spermatid isolated from human testis using the laser capture microdissection technique

Cited by 42 publications

References 21 publications

Doubly uniparental inheritance and highly divergent mitochondrial genomes of the freshwater mussel Unio tumidus (Bivalvia: Unionidae)

Doubly uniparental inheritance and highly divergent mitochondrial genomes of the freshwater mussel Unio tumidus (Bivalvia: Unionidae)

Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 4: Intercellular bridges, mitochondria, nuclear envelope, apoptosis, ubiquitination, membrane/voltage‐gated channels, methylation/acetylation, and transcription factors

Screening of genes involved in chromosome segregation during meiosis I: toward the identification of genes responsible for infertility in humans

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