Identification of differentially expressed circulating exosomal lncRNAs in IgA nephropathy patients

Abstract

Background: Although immunoglobulin A nephropathy (IgAN) is one of the foremost primary glomerular disease, treatment of IgAN is still in infancy. Non-invasive biomarkers are urgently needed for IgAN diagnosis. We investigate the difference in expression profiles of exosomal long non-coding-RNAs (lncRNAs) in plasma from IgAN patients compared with their healthy first-degree relatives, which may reveal novel non-invasive IgAN biomarkers. Methods: We isolated exosomes from the plasma of both IgAN patients and their healthy first-degree relatives. High-throughput RNA sequencing and real-time quantitative polymerase chain reaction (qRT-PCR) was used to validate lncRNA expression profiles. Pathway enrichment analysis was used to predict their nearest protein-coding genes. Results: lncRNA-G21551 was significantly down-regulated in IgAN patients. Interestingly, the nearest protein-coding gene of lncRNA-G21551 was found to be encoding the low affinity receptor of the Fc segment of immunoglobulin G (FCGR3B). Conclusions: Exosomal lncRNA-G21551, with FCGR3B as the nearest protein-coding gene, was down-regulated in IgAN patients, indicating its potential to serve as a non-invasive biomarker for IgAN.

Resistance Training Diminishes the Expression of Exosome CD63 Protein without Modification of Plasma miR-146a-5p and cfDNA in the Elderly

“…Elderly individuals with the severity of coronary stenosis have elevated plasma miR-146a-5p levels [ 42 ]. Furthermore, Guo et al [ 43 ] reported a positive correlation between plasma miR-146a-5p and both carotid intima-media thickness and brachial-ankle pulse wave velocity in middle-aged to elderly diabetic patients. This discrepancy with our findings is most likely due to health status because the current study recruited only elderly individuals without a history of inflammatory and/or metabolic diseases, which may have eliminated some confounding factors compared with young adults.…”

Effects of exercise on exosome release and cargo in in vivo and ex vivo models: A systematic review

“…The potential role of exosomes has been recently investigated not only as less invasive biomarkers in a wide range of diseases, including various cancer (breast [Hannafon et al, 2016], cervix [Zheng et al, 2019], endometrium [Srivastava et al, 2018], gastric [Tang et al, 2020], lung [Cazzoli et al, 2013], pancreatic [Melo et al, 2015], prostate [Barceló et al, 2019], ovaries [Maeda et al, 2020]), Alzheimer's disease (Hamlett et al, 2017), Parkinson's disease (Kitamura et al, 2018), brain insulin resistance (Mullins et al, 2017), diabetes (Krishnan et al, 2019), cardiomyopathies (T. Wu, Chen, et al, 2018), nephropathies (Guo et al, 2020) and chronic kidney disease (Khurana et al, 2017), and multiple sclerosis (Ebrahimkhani et al, 2017), but also as therapeutic agents for both drug delivery vehicles (tumor therapy [Altanerova et al, 2019; Qi et al, 2016; Y. Tian et al, 2014], Parkinson's disease [Haney et al, 2015], cerebral ischemia therapy [T. Tian et al, 2018]) and a genetic or protein material transfer mediator (Alzheimer's disease [Yang et al, 2020], breast cancer [Sansone et al, 2017], ischemic myocardium [Gollmann‐Tepeköylü et al, 2020]) or graft‐versus‐host disease (Kordelas et al, 2014). In this regard, another potential translational perspective could be the transfusion or transferring of exosomes or enriched exosomes.…”

“…diabetes (Krishnan et al, 2019), cardiomyopathies (T. Wu, Chen, et al, 2018), nephropathies (Guo et al, 2020) and chronic kidney disease (Khurana et al, 2017), and multiple sclerosis (Ebrahimkhani et al, 2017), but also as therapeutic agents for both drug delivery vehicles (tumor therapy [Altanerova et al, 2019;Qi et al, 2016;Y. Tian et al, 2014], Parkinson's disease [Haney et al, 2015], cerebral ischemia therapy [T. Tian et al, 2018]) and a genetic or protein material transfer mediator (Alzheimer's disease [Yang et al, 2020], breast cancer [Sansone et al, 2017], ischemic myocardium [Gollmann-Tepeköylü et al, 2020]) or graft-versus-host disease (Kordelas et al, 2014).…”

Contribution of LOC105371267 and MRPS30-DT Genetic Polymorphisms in IgA Nephropathy Among Chinese Han Population 

“…Guo et al used high-throughput RNA sequencing and qRT-PCR to test the exosomes isolated from plasma of IgAN patients and their healthy rst-degree relative. The results revealed that exsomal lncRNA-G21551 was down-regulated in IgAN patients, indicating its potential to serve as a non-invasive biomarker for IgAN [14]. In the study of Zuo et al, peripheral blood mononuclear cells were collected from both IgAN patients and healthy controls to identify differentially expressed lncRNAs and mRNAs by microarray analysis and quantitative polymerase chain reaction.…”