Identification of differentially expressed cDNA sequences and histological characteristics of Hevea brasiliensis calli in relation to their embryogenic and regenerative capacities

Charbit, Erika; Legavre, Thierry; Lardet, Ludovic; Bourgeois, Emmanuelle; Ferrière, Nicole; Carron, Marc-Philippe

doi:10.1007/s00299-003-0737-z

Cited by 30 publications

(20 citation statements)

References 31 publications

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“…For example, hypermethylation of DNA was found to be associated with a lack of organogenic potential in radiata pine micrografts collected from mature versus juvenile trees (Fraga et al 2002a). Other evidence that gene expression was linked to embryogenic potential came from the work by Charbit et al (2004) who distinguished four of 28 complementary DNAs of Hevea brasiliensis (rubber tree) that were differentially expressed in embryogenic and regenerating cultures versus nonembryogenic cultures.…”

Section: Introductionmentioning

confidence: 95%

Biological characterization of young and aged embryogenic cultures of Pinus pinaster (Ait.)

Klimaszewska

Noceda

Pelletier

et al. 2008

In Vitro Cell.Dev.Biol.-Plant

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Pinus pinaster (Ait.) somatic embryogenesis (SE) has been developed during the last decade, and its application in tree improvement programs is underway. Nevertheless, a few more or less important problems still exist, which have an impact on the efficiency of specific SE stages. One phenomenon, which had been observed in embryogenic tissue (embryonal mass, EM) initiated from immature seed, has been the loss of the ability to produce mature somatic embryos after the tissue had been cultured for several months. In an attempt to get insight into the differences between young cultures of EM (3-mo-old since the first subculture) of P. pinaster that produced mature somatic embryos and the same lines of significantly increased age (18-mo-old, aged EM) that stopped producing mature somatic embryos, we analyzed in both types of materials the levels of endogenous hormones, polyamines, the global DNA methylation, and associated methylation patterns. In addition, we included in the analysis secondary EM induced from mature somatic embryos. The analysis showed that the two tested genotypes displayed inconsistent hormonal and polyamine profiles in EM cultures of a similar phenotype and that it might be difficult to attribute one specific profile to a specific culture phenotype among genotypes. Experiments were also undertaken to determine if the global DNA methylation and/or the resulting methylation pattern could be manipulated by treatment of the cultures with a hypomethylating drug 5-azacytidine (5-azaC). An aged EM was exposed to different concentrations and durations of 5-azaC, and its response in culture was established by fresh mass increases and somatic embryo maturation potential. All of the analyses are new in maritime pine, and thus, they provide the first data on the biochemistry of EM in this species related to embryogenic potential.

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Section: Introductionmentioning

confidence: 95%

Biological characterization of young and aged embryogenic cultures of Pinus pinaster (Ait.)

Klimaszewska

Noceda

Pelletier

et al. 2008

In Vitro Cell.Dev.Biol.-Plant

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“…However, this indirect secondary somatic embryogenesis process is restricted to just a few clones and the embryo and plant regeneration capacity is variable. The absence of plant regeneration potential in some friable callus lines has been linked to early vacuolization of cells in the embryogenic globules [34]. Some transcriptional changes have also been reported for certain Hevea callus lines with different embryogenic potentials [34].…”

Section: Introductionmentioning

confidence: 99%

Some ethylene biosynthesis and AP2/ERF genes reveal a specific pattern of expression during somatic embryogenesis in Hevea brasiliensis

et al. 2012

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BackgroundEthylene production and signalling play an important role in somatic embryogenesis, especially for species that are recalcitrant in in vitro culture. The AP2/ERF superfamily has been identified and classified in Hevea brasiliensis. This superfamily includes the ERFs involved in response to ethylene. The relative transcript abundance of ethylene biosynthesis genes and of AP2/ERF genes was analysed during somatic embryogenesis for callus lines with different regeneration potential, in order to identify genes regulated during that process.ResultsThe analysis of relative transcript abundance was carried out by real-time RT-PCR for 142 genes. The transcripts of ERFs from group I, VII and VIII were abundant at all stages of the somatic embryogenesis process. Forty genetic expression markers for callus regeneration capacity were identified. Fourteen markers were found for proliferating calli and 35 markers for calli at the end of the embryogenesis induction phase. Sixteen markers discriminated between normal and abnormal embryos and, lastly, there were 36 markers of conversion into plantlets. A phylogenetic analysis comparing the sequences of the AP2 domains of Hevea and Arabidopsis genes enabled us to predict the function of 13 expression marker genes.ConclusionsThis first characterization of the AP2/ERF superfamily in Hevea revealed dramatic regulation of the expression of AP2/ERF genes during the somatic embryogenesis process. The gene expression markers of proliferating callus capacity to regenerate plants by somatic embryogenesis should make it possible to predict callus lines suitable to be used for multiplication. Further functional characterization of these markers opens up prospects for discovering specific AP2/ERF functions in the Hevea species for which somatic embryogenesis is difficult.

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“…[45]. Until now, some protein markers for embryogenic potential were described [16,24,25,[46][47][48][49][50][51], as well as a structural marker of embryogenic competency and regeneration ability [52]. Two types of protein markers described in the literature are chitinase and glucanase enzymes.…”

Section: Discussionmentioning

confidence: 99%

The role of chitinases and glucanases in somatic embryogenesis of black pine and hybrid firs

et al. 2013

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Glucanase and chitinase enzymes play an important role in different plant processes including defense against pathogens and morphogenesis. Moreover, their role in the processes of somatic embryogenesis has been demonstrated. It has been suggested, that the presence of this type of proteins might be a marker for embryogenic potential of callus cultures. In this work we screened for the presence of glucanases and chitinases in liquid growth media of a set of conifer embryogenic cell lines in order to find correlation with their embryogenic potential. We have found that none of the 12 chitinase isoforms detected in culture media of Pinus nigra Arn. or the nine chitinases detected in media with Abies alba × A. cephalonica and Abies alba × A. numidica embryogenic tissues could be linked to their embryogenic capacity. Similarly, none of the six glucanase isoforms detected in the extracellular fluid of Pinus nigra Arn. cultures can be assigned as a marker of embryogenic potential. Thus, our data indicate the large variability and doubtless importance of glucanases and chitinases for cell growth and development of somatic embryos, however, do not support the premise that they are markers of embryogenesis.

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Identification of differentially expressed cDNA sequences and histological characteristics of Hevea brasiliensis calli in relation to their embryogenic and regenerative capacities

Cited by 30 publications

References 31 publications

Biological characterization of young and aged embryogenic cultures of Pinus pinaster (Ait.)

Biological characterization of young and aged embryogenic cultures of Pinus pinaster (Ait.)

Some ethylene biosynthesis and AP2/ERF genes reveal a specific pattern of expression during somatic embryogenesis in Hevea brasiliensis

The role of chitinases and glucanases in somatic embryogenesis of black pine and hybrid firs

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