Identification of differential signs of squamous cell lung carcinoma by means of the mass spectrometry profiling of blood plasma

Pyatnitskiy, Mikhail A.; Лисица, А. В.; Moshkovskii, Sergei A.; Arnotskaya, N. E.; Ахмедов, Б. Б.; Заридзе, Давид; Полоцкий, Б. Е.; Шевченко, В. Е.

doi:10.1134/s1061934811140139

Cited by 4 publications

(3 citation statements)

References 19 publications

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“…Profiling of blood plasma and bioinformatics tools allowed the selection of 15 mass spectral protein peaks that may potentially serve for the early diagnosis of the disease. 183 To identify potential biomarkers of ovarian cancer, mass spectrometric proteome profiling of tumor pleural effusion (TPE) liquid fraction from patients has been performed. 184 Methodology of analysis of the TPE protein composition included removal of high-abundant proteins by affine chromatography, additional fractionation of the low-abundant proteins based on their lipophilicity and high-resolution mass spectrometric analysis.…”

Section: Medical Chemistrymentioning

confidence: 99%

Mass Spectrometry in Russia

Заикин

Сысоев

2013

Eur J Mass Spectrom (Chichester)

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The present review covers the main research in the area of mass spectrometry from the 1990s which was about the same time as the Russian Federation emerged from the collapse of the Soviet Union (USSR). It consists of two main parts-application of mass spectrometry to chemistry and related fields and creation and development of mass spectrometric technique. Both traditional and comparatively new mass spectrometric methods were used to solve various problems in organic chemistry (reactivity of gas-phase ions, structure elucidation and problems of identification, quantitative and trace analysis, differentiation of stereoisomers, derivatization approaches etc.), biochemistry (proteomics and peptidomics, lipidomics), medical chemistry (mainly the search of biomarkers, pharmacology, doping control), environmental, petrochemistry, polymer chemistry, inorganic and physical chemistry, determination of natural isotope ratio etc. Although a lot of talented mass spectrometrists left Russia and moved abroad after the collapse of the Soviet Union, the vitality of the mass spectral community proved to be rather high, which allowed the continuation of new developments in the field of mass spectrometric instrumentation. They are devoted to improvements in traditional magnetic sector mass spectrometers and the development of new ion source types, to analysis and modification of quadrupole, time-of-flight (ToF) and ion cyclotron resonance (ICR) analyzers. The most important achievements are due to the creation of multi-reflecting ToF mass analyzers. Special attention was paid to the construction of compact mass spectrometers, particularly for space exploration, of combined instruments, such as ion mobility spectrometer/mass spectrometer and accelerating mass spectrometers. The comparatively young Russian Mass Spectrometry Society is working hard to consolidate the mass spectrometrists from Russia and foreign countries, to train young professionals on new appliances and regularly holds conferences on mass spectrometry. For ten years, a special journal Mass-spektrometria has published papers on all disciplines of mass spectrometry.

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Section: Medical Chemistrymentioning

confidence: 99%

Mass Spectrometry in Russia

Заикин

Сысоев

2013

Eur J Mass Spectrom (Chichester)

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“…С помощью времяпролетной масс-спектрометрии с матрично-активированной лазерной десорбцией/ионизацией (MALDI-TOF-MS) можно получить профили экспрессии белков в биологических образцах человека и обнаружить потенциальные биомаркеры рака. Недавно показана эффективность этой технологии для открытия белковых маркеров в сыворотке/плазме крови человека [7][8][9][10][11].…”

Section: Introductionunclassified

Search of potential gastric cancer biomarkers using low molecular weight blood plasma proteome profiling by mass spectrometry

et al. 2014

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Gastric cancer, one of the most widespread malignant tumors, still lacks reliable serum/plasma biomarkers of its early detection. In this study we have developed, unified, and tested a new methodology for search of gastric cancer biomarkers based on profiling of low molecular weight proteome (LMWP) (1-17 kDa). This approach included three main components: sample pre-fractionation, matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS), data analysis by a bioinformatics software package. Applicability and perspectives of the developed approach for detection of potential gastric cancer markers during LMWP analysis have been demonstrated using 69 plasma samples from patients with gastric cancer (stages I-IV) and 238 control samples. The study revealed peptides/polypeptides, which may be potentially used for detection of this pathology.

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“…14 In principle, lung cancer specific peptides/proteins would be released in the blood and be detectable with highly sensitive and specific methods, allowing the diagnosis of the disease even in the presence of very small tumors, potentially still at an early and curable stage. [15][16][17][18][19] To improve the performance of plasma/serum proteomics, several pre-fractionation strategies have been developed, sometimes in combination with shotgun liquid chromatography tandem mass spectrometry (LC-MS/MS) approaches. [17][18][19] However, studies including large numbers of patients are still lacking at present.…”

mentioning

confidence: 99%

Human Blood Plasma Proteome Mapping for Search of Potential Markers of the Lung Squamous Cell Carcinoma

Шевченко

Ковалев

Arnotskaya

et al. 2013

Eur J Mass Spectrom (Chichester)

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Blood plasma proteomes obtained from 77 lung squamous cell carcinoma (LSCC) patients (Stages I-III) and 67 healthy controls (all males) were analyzed by using the label-free liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the search of potential cancer biomarkers. All plasma samples were depleted of 14 highly-abundant plasma proteins by immune-affinity column chromatography before LC-MS/MS. We identified and quantified 809 differential proteins with molecular weights from 6.4 kDa to 3900 kDa using a label-free method. Three hundred and sixty four proteins were identified in all three groups. Changes in levels of an expression of blood plasma proteins associated with LSCC were discovered. Among them, 43 proteins were overexpressed and 39 proteins were down-regulated by more than two-fold between the plasmas of lung cancer patients and healthy men. We focused our attention on proteins whose expression levels increased from control to early stage and then to advanced stage tumor. Each of the 43 unique overexpressed proteins was classified according to its cellular localization, biological processes, molecular function and classes. Many of these proteins are involved in biological pathways pertinent to tumor progression and metastasis and some of these deregulated proteins may be useful clinical markers.

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Identification of differential signs of squamous cell lung carcinoma by means of the mass spectrometry profiling of blood plasma

Cited by 4 publications

References 19 publications

Mass Spectrometry in Russia

Mass Spectrometry in Russia

Search of potential gastric cancer biomarkers using low molecular weight blood plasma proteome profiling by mass spectrometry

Human Blood Plasma Proteome Mapping for Search of Potential Markers of the Lung Squamous Cell Carcinoma

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