Identification of Different Phenotypes of Interstitial Cells in the Upper and Deep Lamina Propria of the Human Bladder Dome

Gevaert, Thomas; Vanstreels, Els; Daelemans, Dirk; Franken, Jan; Aa, Frank Van der; Roskams, Tania; Ridder, Dirk De

doi:10.1016/j.juro.2014.05.096

Cited by 50 publications

(72 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…the area between the urothelium and detrusor smooth muscle) and IC in the detrusor [4] (inter-and intramuscular). Having been reported in bladders from different species including rat [5], guinea pig [4,6], mouse [7] and human beings [8], these two IC populations appear to be a constant, species-independent finding. Functional data on bladder IC are still limited, and the general hypothesis is that LP IC might be involved in non-neural sensory signal transduction from the urothelium towards the detrusor muscle, while detrusor IC might have a role in pacemaking and/or transduction of pacemaking signals in the detrusor area [3].…”

Section: Introductionmentioning

confidence: 79%

“…Several authors also reported on the absence of the typical ultrastructural ICC characteristics in IC in the bladder, lending additional evidence for another phenotype for these cells [8,18,19]. We know from previous work that IC in the bladder constitute a complex cell family including fibroblasts, myofibroblasts, ICC-like cells and telocytes, with different IC phenotypes in the lamina propria versus the detrusor [8,18,19]. At present, our knowledge of Lower panels: immunohistochemical stains for KIT on guinea pig bladder and gut with clone A4502.…”

Section: Kitmentioning

confidence: 89%

See 1 more Smart Citation

The stem cell growth factor receptor KIT is not expressed on interstitial cells in bladder

Gevaert¹,

Vanstreels²,

Daelemans³

et al. 2017

European Urology Supplements

View full text Add to dashboard Cite

The mast/stem cell growth factor receptor KIT has long been assumed to be a specific marker for interstitial cells of Cajal (ICC) in the bladder, with possible druggable perspectives. However, several authors have challenged the presence of KIT + ICC in recent years. The aim of this study was therefore to attempt to clarify the conflicting reports on KIT expression in the bladder of human beings, rat, mouse and guinea pig and to elucidate the possible role of antibody-related issues and interspecies differences in this matter. Fresh samples were obtained from human, rat, mouse and guinea pig cystectomies and processed for single/double immunohistochemistry/immunofluorescence. Specific antibodies against KIT, mast cell tryptase (MCT), anoctamin-1 (ANO1) and vimentin were used to characterize the cell types expressing KIT. Gut (jejunum) tissue was used as an external antibody control. Our results revealed KIT expression on mast cells but not on ICC in human, rat, mouse and guinea pig bladder. Parallel immunohistochemistry showed KIT expression on ICC in human, rat, mouse and guinea pig gut, which confirmed the selectivity of the KIT antibody clones. In conclusion, we have shown that KIT + cells in human, rat, mouse and guinea pig bladder are mast cells and not ICC. The present report is important as it opposes the idea that KIT + ICC are present in bladder. In this perspective, functional concepts of KIT + ICC being involved in sensory and/or motor aspects of bladder physiology should be revised.

show abstract

Section: Introductionmentioning

confidence: 79%

Section: Kitmentioning

confidence: 89%

The stem cell growth factor receptor KIT is not expressed on interstitial cells in bladder

Gevaert¹,

Vanstreels²,

Daelemans³

et al. 2017

European Urology Supplements

View full text Add to dashboard Cite

show abstract

“…The role this compact subepithelial layer has received considerable attention lately, having been proposed to modulate afferent discharge and sensations [10, 11]. Whether these cells are ICs with a different phenotype [30] or fibroblasts with myoid differentiation, given the expression of α -smooth muscle actin [34], awaits further investigations. In the present study, these suburothelial ICs in the bladder and urethra were not affected by either CYP or Imatinib treatment.…”

Section: Discussionmentioning

confidence: 99%

Proliferation of Interstitial Cells in the Cyclophosphamide-Induced Cystitis and the Preventive Effect of Imatinib

Sancho

Triguero

Lafuente-Sanchis

et al. 2017

BioMed Research International

View full text Add to dashboard Cite

Cyclophosphamide- (CYP-) induced cystitis in the rat is a well-known model of bladder inflammation that leads to an overactive bladder, a process that appears to involve enhanced nitric oxide (NO) production. We investigated the changes in the number and distribution of interstitial cells (ICs) and in the expression of endothelial NO synthase (eNOS) in the bladder and urethra of rats subjected to either intermediate or chronic CYP treatment. Pronounced hyperplasia and hypertrophy of ICs were evident within the lamina propria and in the muscle layer. IC immunolabeling with CD34, PDGFRα, and vimentin was enhanced, as reflected by higher colocalization indexes of the distinct pairs of markers. Moreover, de novo expression of eNOS was evident in vimentin and CD34 positive ICs. Pretreatment with the receptor tyrosine kinase inhibitor Imatinib prevented eNOS expression and ICs proliferation, as well as the increased voiding frequency and urinary tract weight provoked by CYP. As similar results were obtained in the urethra, urethritis may contribute to the uropathology of CYP-induced cystitis.

show abstract

“…There is also evidence of 2 main interstitial cell populations in the upper and deeper lamina propria in the human bladder dome region, which have distinct ultrastructural and immunohistochemical phenotypes [20]. This differentiation in detrusor muscle development and ultrastructural composition may play a role in the pathophysiology associated with IC.…”

Section: Discussionmentioning

confidence: 99%

A Feasibility Study to Determine Whether Clinical Contrast Enhanced Magnetic Resonance Imaging can Detect Increased Bladder Permeability in Patients with Interstitial Cystitis

Towner

Wisniewski

et al. 2016

Journal of Urology

View full text Add to dashboard Cite

Purpose Interstitial cystitis/bladder pain syndrome (IC/BPS) is a bladder pain disorder associated with voiding symptomatology and other systemic chronic pain disorders. Currently diagnosis of IC/BPS is complicated, as patients present with wide ranges of symptoms, physical examination findings, and clinical test responses. One hypothesis is that IC symptoms arise from increased bladder permeability to urine solutes. This study established the feasibility of using contrast-enhanced magnetic resonance imaging (CE-MRI) to quantify bladder permeability in IC patients. Materials and Methods Permeability alterations in bladder urothelium were assessed with intravesical administration of a MRI contrast agent (Gd-DTPA) in a small cohort of patients. MRI signal intensities (SI) in IC patient and control bladders were compared regionally and for entire bladders. Results Quantitative assessment of MRI SI indicated a significant increase in SI within anterior bladder regions (p<0.01) compared to posterior regions in IC patients, and significant increases in SI within anterior bladder regions (p<0.001) and kurtosis (descriptor of shape of probability distribution) and skewness (measure of asymmetry of probability distribution) associated with contrast enhancement in total bladders (p<0.05) for IC patients compared to controls. Regarding symptomatology, IC cases differed significantly from controls for the SF-36, PPUF and ICPI questionnaires with no overlap in range of scores for each group, and were significantly different for ICSI but with a slight overlap in range of scores. Conclusions The data suggests that CE-MRI provides an objective, quantifiable measurement of bladder permeability that could be used to stratify bladder pain patients and monitor therapy.

show abstract

Identification of Different Phenotypes of Interstitial Cells in the Upper and Deep Lamina Propria of the Human Bladder Dome

Cited by 50 publications

References 30 publications

The stem cell growth factor receptor KIT is not expressed on interstitial cells in bladder

The stem cell growth factor receptor KIT is not expressed on interstitial cells in bladder

Proliferation of Interstitial Cells in the Cyclophosphamide-Induced Cystitis and the Preventive Effect of Imatinib

A Feasibility Study to Determine Whether Clinical Contrast Enhanced Magnetic Resonance Imaging can Detect Increased Bladder Permeability in Patients with Interstitial Cystitis

Contact Info

Product

Resources

About