Identification of deoxynucleoside—polyaromatic hydrocarbon adducts by capillary zone electrophoresis—Continuous Flow-fast atom bombardment mass spectrometry

Wolf, Susan M.; Vouros, Paul; Norwood, C.B.; Jackim, Eugene

doi:10.1016/1044-0305(92)87089-h

Cited by 27 publications

(14 citation statements)

References 32 publications

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“…In one of the earliest examples, characterization of a mixture of several deoxynucleoside adducts of different polycyclic aromatic hydrocarbons by CZE-CF-FAB-MS was demonstrated by Wolf et al [74]. The adducts were formed by covalent attachment of four PAH and amino-PAH compounds to 2'-deoxyguanosine (dG).…”

Section: Dna Adductsmentioning

confidence: 98%

“…Therefore, separation of the modified and the unmodified nucleotides as well as within the modified nucleotide group offers the method the much needed selectivity. Nowadays, capillary LC, nano-LC [68][69][70][71][72][73], and CZE [57,[59][60][61][74][75][76][77][78][79][80][81][82][83][84][85][86][87] are more and more used as separation techniques for the analysis of DNA adducts and have proven to allow a very sensitive analysis procedure, e.g., in conjunction with mass spectrometric detection. In the elucidation of DNA adducts, ESI is currently the most used ionization technique when interfacing LC or CZE separations to MS.…”

Section: Dna Adductsmentioning

confidence: 99%

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Analysis of nucleic acid constituents by on‐line capillary electrophoresis‐mass spectrometry

et al. 2005

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This review is focused on the capillary electrophoresis-mass spectrometric (CE-MS) analysis of nucleic acid constituents in the broadest sense, going from nucleotides and adducted nucleotides over nucleoside analogues to oligonucleotides. These nucleic acid constituents play an important role in a variety of biochemical processes. Hence, their isolation, identification, and quantification will undoubtedly help reveal the process of life and disease mechanisms, such as carcinogenesis, and can also be useful for antitumor and antiviral drug research to provide valuable information about mechanism of action, pharmacokinetics, pharmacodynamics, toxicity, therapeutic drug level monitoring, and quality control related to this substance class. Fundamental investigations into their structure, the search for modifications, the occurrence and biochemical impact of structural variation amongst others, are therefore of great value. In view of the related bioanalytical procedures, the coupling of CE to MS has emerged as a powerful tool for the analysis of the complex mixtures of nucleic acid constituents: CE confers rapid analysis and efficient resolution, while MS provides high selectivity and sensitivity with structural characterization of minute amounts of compound. After an introduction about the biochemical and analytical perspectives on the nucleic acid constituents, the different modes of CE used in this field of research as well as the relevant CE-MS interfaces and the difficulties associated with quantitative CE-MS are briefly discussed. A large section is finally devoted to field-oriented applications.

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Section: Dna Adductsmentioning

confidence: 98%

Section: Dna Adductsmentioning

confidence: 99%

Analysis of nucleic acid constituents by on‐line capillary electrophoresis‐mass spectrometry

et al. 2005

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“…The authors concluded that, even though the adduct levels that can be detected with CE-MS are still several orders of magnitude higher than with the standard 32P-post-labeling technique, the possibility of identification justifies its parallel use. The group of Vouros [166] successfully analyzed PAH and amino-PAH adducts to deoxyguanosine by CE-FAB-MS. They could easily detect as low as 63 pg of synthetic PAH-DNA adduct by monitoring the signals arising from the loss of deoxyribose from the MH+ ion.…”

Section: Nucleotidesmentioning

confidence: 99%

Analyte identification in capillary electrophoretic separation techniques

et al. 1998

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A review on applications of on-line hyphenation in capillary electrophoresis and capillary electrochromatography for the identification of migrating analytes is presented. There is an urgent need for unambiguous analyte identification by combining spectral information and observed migration times, because the parameters influencing the migration times and separation efficiencies in these separation techniques are not easily controlled, especially when real samples containing unknown interferences have to be analyzed. The spectrometric techniques covered here are ultraviolet and visible radiation (UV/Vis) absorption, fluorescence including fluorescence line-narrowing spectroscopy, Raman spectroscopy, nuclear magnetic resonance and mass spectrometry. Attention is essentially confined to literature reports in which the extra information provided by the detector is really used for identification purposes, especially in real-life samples, while the interfacing as such and analyte detectabilities in standard solutions are only briefly discussed. This article covers an extensive fraction of the literature published on this topic until the beginning of 1998.

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“…The CE-CF-FAB systems reported in literature have all been based on either the liquid-junction (Minard et al, 1988;Reinhoud et al, 1989Reinhoud et al, , 1990Wolf et al, 1992) or the sheathflow design (Caprioli et al, 1989;Moseley, Deterding, & Tomer, 1989a,b;Moseley et al, 1990Moseley et al, , 1991Wolf & Vouros, 1995). The feasibility of CE-CF-FAB-MS was first reported by Minard et al (1988) with a liquid-junction interface.…”

Section: B Cf-fabmentioning

confidence: 99%

“…Initial liquid-junction interfaces were found to result in significant band-broadening and plate numbers were often limited to 10,000 theoretical plates (Minard et al, 1988;Reinhoud et al, 1989). Modification of the interface design by Caprioli et al (1989) and Wolf et al (1992) led to significant improvements in CE separation efficiency.…”

Section: B Cf-fabmentioning

confidence: 99%

Ionization techniques in capillary electrophoresis‐mass spectrometry: Principles, design, and application

Hommerson

Khan

Jong

et al. 2011

Mass Spectrometry Reviews

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A major step forward in the development and application of capillary electrophoresis (CE) was its coupling to ESI-MS, first reported in 1987. More than two decades later, ESI has remained the principal ionization technique in CE-MS, but a number of other ionization techniques have also been implemented. In this review the state-of-the-art in the employment of soft ionization techniques for CE-MS is presented. First the fundamentals and general challenges of hyphenating conventional CE and microchip electrophoresis with MS are outlined. After elaborating on the characteristics and role of ESI, emphasis is put on alternative ionization techniques including sonic spray ionization (SSI), thermospray ionization (TSI), atmospheric pressure chemical ionization (APCI), atmospheric pressure photoionization (APPI), matrix-assisted laser desorption ionization (MALDI) and continuous-flow fast atom bombardment (CF-FAB). The principle of each ionization technique is outlined and the experimental set-ups of the CE-MS couplings are described. The strengths and limitations of each ionization technique with respect to CE-MS are discussed and the applicability of the various systems is illustrated by a number of typical examples.

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Identification of deoxynucleoside—polyaromatic hydrocarbon adducts by capillary zone electrophoresis—Continuous Flow-fast atom bombardment mass spectrometry

Cited by 27 publications

References 32 publications

Analysis of nucleic acid constituents by on‐line capillary electrophoresis‐mass spectrometry

Analysis of nucleic acid constituents by on‐line capillary electrophoresis‐mass spectrometry

Analyte identification in capillary electrophoretic separation techniques

Ionization techniques in capillary electrophoresis‐mass spectrometry: Principles, design, and application

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