Identification of cytochrome P450 monooxygenase genes and their expression profiles in cyhalothrin-treated Colorado potato beetle, Leptinotarsa decemlineata

Wan, Pin-Jun; Shi, Xiaoqin; Kong, Ye; Zhou, Lecong; Guo, Wen‐Chao; Ahmat, Tursun; Li, Guoqing

doi:10.1016/j.pestbp.2013.10.004

Cited by 61 publications

(39 citation statements)

References 66 publications

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“…We individually silenced genes coding for prothoracicotropic hormone (PTTH)–Torso signaling components ( LdPTTH and LdTorso ) and ecdysteroidogenesis enzymes ( Phantom ( LdPHM ) and Shade ( LdSHD ); Wan et al ., ; Kong et al ., ; Zhu et al ., ) by dietary introduction of dsRNA (Fig. S1), and found that the messenger RNA (mRNA) levels of both LdCDA2a and LdCDA2b were significantly decreased in these RNAi larvae, compared with those in the controls (Fig.…”

Section: Resultsmentioning

confidence: 98%

“…Specific primers used to clone the fragments of dsRNAs are listed in Table S1. These dsRNAs were individually expressed using Escherichia coli HT115 (DE3) competent cells lacking RNase III following the established method (Wan et al ., ). Individual colonies were inoculated, and then grown until cultures reached an OD600 value of 1.0.…”

Section: Methodsmentioning

confidence: 97%

“…The same method as previously reported (Wan et al ., ) was used to introduce dsRNA into larvae. Potato leaves were immersed with a bacterial suspension containing a dsRNA for 5 s, removed, and dried for 2 h under airflow on filter paper.…”

Section: Methodsmentioning

confidence: 99%

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Silencing chitin deacetylase 2 impairs larval–pupal and pupal–adult molts in Leptinotarsa decemlineata

Chen

Wang

et al. 2018

Insect Molecular Biology

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Insect chitin deacetylases (CDAs) are carbohydrate esterases that catalyze N-deacetylation of chitin to generate chitosan, a process essential for chitin organization and compactness during the formation of extracellular chitinous structure. Here we identified two CDA2 splice variants (LdCDA2a and LdCDA2b) in Leptinotarsa decemlineata. Both splices were abundantly expressed in larval foregut, rectum, and epidermis; their levels peaked immediately before ecdysis within each instar. In vivo results revealed that the two isoforms transcriptionally responded, positively and negatively respectively, to 20-hydroxyecdysone and juvenile hormone signaling pathways. RNA interference (RNAi)-aided knockdown of the two LdCDA2 variants (hereafter LdCDA2) or LdCDA2b, rather than LdCDA2a, resulted in three negative effects. First, foliage consumption was significantly reduced, larval developing period was lengthened, and larval growth was retarded. Second, chitin contents were reduced, whereas glucose, trehalose, and glycogen contents were increased in the LdCDA2 and LdCDA2b RNAi larvae. Third, approximately 20% of LdCDA2 and LdCDA2b RNAi larvae were trapped within the exuviae and finally died. About 60% of the abnormal pupae died as pharate adults. Around 20% of the RNAi pupae emerged as deformed adults, with small size and wrinkled wings. These adults eventually died within 1 week after molting. Our results reveal that knockdown of CDA2 affects chitin accumulation. Consequently, LdCDA2 may be a potential target for control of L. decemlineata larvae.

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Section: Resultsmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 97%

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Silencing chitin deacetylase 2 impairs larval–pupal and pupal–adult molts in Leptinotarsa decemlineata

Chen

Wang

et al. 2018

Insect Molecular Biology

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“…The expressed sequence tags (ESTs) of the putative AS and JHAMT genes were obtained from the L. decemlineata transcriptome data Swevers et al, 2013;Wan et al, 2013;Kumar et al, 2014). To verify the correctness of the sequence, total RNA was extracted from the fourth instars and first-strand cDNA was synthesized using the reverse transcriptase (M-MLV RT) (Takara Bio., Dalian, China) and an oligo (dT)18 primer, and was used as a template to perform polymerase chain reaction (PCR) using primers in Table 1.…”

Section: Molecular Cloning and Phylogenetic Analysismentioning

confidence: 99%

Involvement of a putative allatostatin in regulation of juvenile hormone titer and the larval development in Leptinotarsa decemlineata (Say)

Meng

Liu

Lü

et al. 2015

Gene

Self Cite

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“…In the Colorado potato beetle Leptinotarsa decemlineata , the most important insect defoliator of potatoes, the main ecdysteroidogenesis (Wan et al , ; Kong et al , ) and 20E signalling (Ogura et al , ; Guo et al , ; Guo et al , ; Xu et al , ; ) genes have been identified. In the present paper, we intended to address the two questions.…”

Section: Introductionmentioning

confidence: 99%

Functional characterization of ultraspiracle in Leptinotarsa decemlineata using RNA interference assay

Deng

et al. 2019

Insect Molecular Biology

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A heterodimer of ultraspiracle (USP) and ecdysone receptor (EcR) mediates 20‐hydroxyecdysone (20E) signalling cascade to regulate insect moulting and metamorphosis. However, at least two questions remain to be addressed in terms of the molecular importance of USP in insect species. First, is USP involved in both regulation of ecdysteroidogenesis and mediation of 20E signalling in non‐drosophilid insects, as in Drosophila melanogaster? Second, does USP play any role in larval metamorphosis except as the partner of heterodimeric receptor to activate the downstream 20E signalling genes? In this paper, we found that RNA interference (RNAi) of LdUSP in the final (fourth) instar larvae reduced the messenger RNA levels of four ecdysteroidogenesis genes (Ldspo, Ldphm, Lddib and Ldsad) and 20E titre, and repressed the expression of five 20E signal genes (EcRA, HR3, HR4, E74 and E75) in Leptinotarsa decemlineata. The LdUSP RNAi larvae remained as prepupae, with developing antennae, legs and discs of forewings and hindwings. Dietary supplement with 20E restored the expression of the five 20E signal genes, but only partially alleviated the decreased pupation rate in LdUSP RNAi beetles. Knockdown of LdUSP at the penultimate (third) instar larvae did not affect third–fourth instar moulting. However, silencing LdUSP caused similar but less severe impairments on pupation. Accordingly, we propose that USP is undoubtedly necessary for ecdysteroidogenesis, for mediation of 20E signalling and for initiation of metamorphosis in L. decemlineata.

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Identification of cytochrome P450 monooxygenase genes and their expression profiles in cyhalothrin-treated Colorado potato beetle, Leptinotarsa decemlineata

Cited by 61 publications

References 66 publications

Silencing chitin deacetylase 2 impairs larval–pupal and pupal–adult molts in Leptinotarsa decemlineata

Silencing chitin deacetylase 2 impairs larval–pupal and pupal–adult molts in Leptinotarsa decemlineata

Involvement of a putative allatostatin in regulation of juvenile hormone titer and the larval development in Leptinotarsa decemlineata (Say)

Functional characterization of ultraspiracle in Leptinotarsa decemlineata using RNA interference assay

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