Identification of Cultivable Microorganisms from Primary Teeth with Necrotic Pulps

Abstract

The objective of this study was to identify cultivable microorganisms from primary teeth with necrotic pulps. This experimental study included 21 patients of both sexes between 4 and 7 years of age with necrotic pulps in primary teeth. Twenty-one maxillary and mandibular molars containing at least 1 necrotic canal, an abscess or sinus tract, one or more radiolucent areas in the furcation or periapical region, teeth having at least two thirds of root length, and carious lesions directly exposed to the oral environment were included. After antisepsis of the oral cavity, anesthesia of the affected tooth, and isolation and disinfection of the operative field, 3 sterile absorbent paper points were sequentially placed for 30 seconds for the collection of samples. The samples were immediately processed in an anaerobic chamber, and all isolated microorganisms were identified. Anaerobic species (anaerobic facultative and moderate anaerobes) were isolated in all root canals; 68.4% of root canal samples studied showed a polymicrobial nature. Most of the isolate consisted of Bifidobacterium Spp2 and Streptococcus intermedius. Other less frequently encountered species were Actinomyces israelii, Bifidobacterium spp 1, Clostridium spp, and Candida albicans. Results indicate the existence of combinations of bacterial species in root canal infections of the primary dentition with necrotic pulps, anaerobic bacteria predominating.

Scardovia wiggsiae and its potential role as a caries pathogen

“…In another study, Parascardovia denticolens was cultured from the forefront of carious lesions with vitally exposed pulps suggesting this Scardovia- related species was associated with lesion progression in dentin [39]. In another study, the major taxon cultured from deciduous pulps was S. wiggsiae ( Bifidobacterium Ssp2 was S. wiggsiae by 16S rRNA sequences) [40]. In a pyrosequencing study, increased relative abundance of Scardovia was higher in dentin caries compared to caries-free sites or initial carious lesions [41].…”

Anaerobic culture to detect periodontal and caries pathogens

“…A separate culture study of infected pulps in children identified high proportions of S. wiggsiae (as Bifidobacterium spp. 2) [68] suggesting that this species is a significant part of the microbial complex involved in the progression of deep dentinal caries.…”

Comparing Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Phenotypic and Molecular Methods for Identification of Species within the Streptococcus anginosus Group

“…These organisms may be nonhemolytic or display beta-or alphahemolysis when grown on blood agar plates (4, 5, 10), and they may present any of the A, C, F, or G Lancefield group antigens or lack Lancefield group antigen altogether (4,5,8,10). SAG, as with other members of the viridans group streptococci, are part of the human microbiota, colonizing the oral cavity, nasopharynx, and gastrointestinal and genitourinary tracts (4,(11)(12)(13). However, these bacteria may also cause infections that can range from mild, such as pharyngitis, to severe, such as bacteremia and abscesses in internal organs, with the three SAG species tending to be associated with different clinical syndromes.…”

“…SAG, as with other members of the viridans group streptococci, are part of the human microbiota, colonizing the oral cavity, nasopharynx, and gastrointestinal and genitourinary tracts (4,(11)(12)(13). However, these bacteria may also cause infections that can range from mild, such as pharyngitis, to severe, such as bacteremia and abscesses in internal organs, with the three SAG species tending to be associated with different clinical syndromes.…”