Identification of CTX-M β-lactamases among Escherichia coli from the community in New York City

Tiruvury, Hemavarna; Johnson, James R.; Mariano, Noriel; Grenner, Louise; Colon-Urban, Rita; Erritouni, Mohamed; Wehbeh, Wehbeh; Segal‐Maurer, Sorana; Rahal, James J.; Johnston, Brian; Urban, Carl

doi:10.1016/j.diagmicrobio.2011.11.008

Cited by 15 publications

(14 citation statements)

References 11 publications

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“…We have previously used other molecular techniques to characterize β-lactamase encoding genes in 24% of the isolates analyzed in this study [7] [8] [22] [23] LaBombardi VJ, unpublished results]. However, recent studies have demonstrated sensitivities and specificities of 100% and accuracies of 94% for the detection of known resistance genes using the Check-Points MDR CT103 XL microarray kit (4,5).…”

Section: Discussionmentioning

confidence: 99%

Detection and Characterization of <i>β</i>-Lactamase Encoding Genes in Carbapenem Non-Susceptible Gram-Negative Bacteria and Susceptibility of Isolates to Ceftazidime-Avibactam at a New York City Community Hospital

Urban¹,

Colon-Urban²,

LaBombardi³

et al. 2016

OJMM

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A surveillance study was undertaken to identify prominent β-lactamase encoding genes in 131 carbapenem non-susceptible gram-negative clinical isolates at a New York City community hospital. KPC carbapenemases were detected in 89% of Enterobacteriaceae as well as additional TEM, SHV, and CTX-M class A enzymes. OXA-23 and OXA-24 were the prevalent class D carbapenemases identified in Acinetobacter species. One OXA-23 in M. morganii and one OXA-48 in K. pneumoniae were also identified. Among class C β-lactamases CMY, ACT/MIR, DHA, and FOX were detected. The in vitro activity of ceftazidime-avibactam by E-test methodology was tested with minimal inhibitory concentrations (MIC) of ≤3 µg/ml for 97.8% of all Enterobacteriaceae, MIC 50/90 of 16/>256 µg/ml for carbapenem non-susceptible Acinetobacter, and 3/6 µg/ml for carbapenem non-susceptible Pseudomonas aeruginosa. Periodic surveillance of isolates to characterize current and emerging β-lactamase genotypes present in local isolates may help identify outbreak situations, provide assistance to infection control and antibiotic stewardship programs, and potentially improve patient outcomes.

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Section: Discussionmentioning

confidence: 99%

Detection and Characterization of <i>β</i>-Lactamase Encoding Genes in Carbapenem Non-Susceptible Gram-Negative Bacteria and Susceptibility of Isolates to Ceftazidime-Avibactam at a New York City Community Hospital

Urban¹,

Colon-Urban²,

LaBombardi³

et al. 2016

OJMM

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“…Accordingly, to ensure appropriate empirical therapy, ED physicians may need to consider the possibility of community-acquired ESBLs, even in pediatric patients who lack identifiable risk factors, especially in high-prevalence locales for ESBL-producing E. coli (11,12).…”

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confidence: 99%

Community-Associated Escherichia coli Harboring CTX-M β-Lactamases from Urine Cultures from Pediatric Patients

Woods¹,

Johnson²,

Padkowsky³

et al. 2012

Antimicrob Agents Chemother

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“…The second objective was to assess the association of particular CTX-M enzymes with the clonal background of strains. The third objective was to compare the distribution of the ST131 isolates with that of the non-ST131 isolates with regard to medical wards, clinical samples, and antibiotic susceptibility in order to better understand the epidemiology of this prevalent clone that often produces CTX-M-15 enzymes (1,6,7,11,15,25,26,30). …”

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Phylogenetic Distribution of CTX-M- and Non-Extended-Spectrum-β-Lactamase-Producing Escherichia coli Isolates: Group B2 Isolates, Except Clone ST131, Rarely Produce CTX-M Enzymes

et al. 2012

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i Escherichia coli is the species most frequently associated with clinical infections by extended-spectrum-␤-lactamase (ESBL)-producing isolates, with the CTX-M ESBL enzymes being predominant and found in genetically diverse E. coli isolates. The main objective of this study was to compare, on the basis of a case-control design, the phylogenetic diversity of 152 CTX-M-producing and 152 non-ESBL-producing clinical E. coli isolates. Multilocus sequence typing revealed that even though CTX-M enzymes were largely disseminated across the diversity of E. coli isolates, phylogenetic group B2 showed a particularly heterogeneous situation. First, clone ST131 of group B2 was strongly associated with CTX-M production (55 [79%] of 70 isolates), with CTX-M-15 being predominant. Second, the remaining members of group B2 were significantly less frequently associated with CTX-M production (9 [12%] of 75) than E. coli phylogenetic groups A, B1, and D (88 [55%] of 159). CTX-M-producing ST131 E. coli isolates were significantly more frequent in patients hospitalized in geriatric wards or long-term care facilities. Besides, the non-ESBL ST131 isolates significantly more frequently showed resistance to penicillins than the non-ESBL, non-ST131 isolates did. In conclusion, the present study emphasizes the particular antimicrobial resistance and epidemiologic characteristics of clone ST131 within group B2, which could result from the higher antibiotic exposure of this clone, as it is the predominant clone of group B2 carried in the human gut. E scherichia coli is the Enterobacteriaceae species that causes the largest number of infections, but this species is also a widespread gut commensal of humans and animals. Strains of E. coli are therefore frequently exposed to antimicrobial agents, which has resulted in the emergence and rapid diffusion of antibioticresistant E. coli clinical isolates. Extended-spectrum ␤-lactamases (ESBLs), which confer resistance to extended-spectrum cephalosporins, were, until the year 2000, produced essentially by Klebsiella pneumoniae and Enterobacter sp. isolates responsible for nosocomial infections. However, E. coli has become dominant among the ESBL-producing enterobacterial species. This is especially worrisome as the human gut carriage of E. coli favors the dissemination of ESBLs in the community (31). Interestingly, the switch of dominant species among the ESBL-producing enterobacterial isolates occurred concomitantly with the emergence of novel ESBL enzymes that belong to the CTX-M family. The CTX-M enzymes have superseded the TEM and SHV ESBLs (2) and are found in genetically diverse E. coli isolates. Among CTX-M enzymes, CTX-M-15 predominates and has been associated with the dissemination of a particular E. coli clone of sequence type 131 (ST131) (23,27).The species E. coli is genetically diverse, and strains have been classified into a number of phylogenetic groups, the most frequent ones being A, B1, B2, and D (12, 14, 29, 32). The ST131 clone belongs to phylogenetic group B2 and is associated wi...

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Identification of CTX-M β-lactamases among Escherichia coli from the community in New York City

Cited by 15 publications

References 11 publications

Detection and Characterization of <i>β</i>-Lactamase Encoding Genes in Carbapenem Non-Susceptible Gram-Negative Bacteria and Susceptibility of Isolates to Ceftazidime-Avibactam at a New York City Community Hospital

Detection and Characterization of <i>β</i>-Lactamase Encoding Genes in Carbapenem Non-Susceptible Gram-Negative Bacteria and Susceptibility of Isolates to Ceftazidime-Avibactam at a New York City Community Hospital

Community-Associated Escherichia coli Harboring CTX-M β-Lactamases from Urine Cultures from Pediatric Patients

Phylogenetic Distribution of CTX-M- and Non-Extended-Spectrum-β-Lactamase-Producing Escherichia coli Isolates: Group B2 Isolates, Except Clone ST131, Rarely Produce CTX-M Enzymes

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Identification of CTX-M β-lactamases among Escherichia coli from the community in New York City

Cited by 15 publications

References 11 publications

Detection and Characterization of &lt;i&gt;β&lt;/i&gt;-Lactamase Encoding Genes in Carbapenem Non-Susceptible Gram-Negative Bacteria and Susceptibility of Isolates to Ceftazidime-Avibactam at a New York City Community Hospital

Detection and Characterization of &lt;i&gt;β&lt;/i&gt;-Lactamase Encoding Genes in Carbapenem Non-Susceptible Gram-Negative Bacteria and Susceptibility of Isolates to Ceftazidime-Avibactam at a New York City Community Hospital

Community-Associated Escherichia coli Harboring CTX-M β-Lactamases from Urine Cultures from Pediatric Patients

Phylogenetic Distribution of CTX-M- and Non-Extended-Spectrum-β-Lactamase-Producing Escherichia coli Isolates: Group B2 Isolates, Except Clone ST131, Rarely Produce CTX-M Enzymes

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Detection and Characterization of <i>β</i>-Lactamase Encoding Genes in Carbapenem Non-Susceptible Gram-Negative Bacteria and Susceptibility of Isolates to Ceftazidime-Avibactam at a New York City Community Hospital

Detection and Characterization of <i>β</i>-Lactamase Encoding Genes in Carbapenem Non-Susceptible Gram-Negative Bacteria and Susceptibility of Isolates to Ceftazidime-Avibactam at a New York City Community Hospital