Identification of csypyrone B1 as the novel product of Aspergillus oryzae type III polyketide synthase CsyB

Seshime, Yasuyo; Juvvadi, Praveen R.; Kitamoto, Katsuhiko; Ebizuka, Yutaka; Fujii, Isao

doi:10.1016/j.bmc.2010.04.058

Cited by 45 publications

(35 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Seshime et al proposed that this product is biosynthesized by condensation of succinyl-CoA and three acetyl/malonyl-CoAs, and the following pyrone ring cyclization. Because the expression of CsyB in E. coli has not been successful so far, the precise biosynthetic mechanism remains unclear (44). In vitro studies are required to further decipher the unusual mechanism of this enzyme.…”

Section: Fungal Type III Pkssmentioning

confidence: 99%

Type III polyketide synthases in natural product biosynthesis

et al. 2012

View full text Add to dashboard Cite

SummaryPolyketides represent an important class of biologically active and structurally diverse compounds in nature. They are synthesized from acyl-coenzyme A substrates by polyketide synthases (PKSs). PKSs are classified into three groups: types I, II, and III. This article introduces recent studies on type III PKSs identified from plants, bacteria, and fungi, and describes the catalytic functions of these enzymes in detail. Plant type III PKSs have been widely studied, as exemplified by chalcone synthase, which plays an important role in the synthesis of plant metabolites. Bacterial type III PKSs fall into five groups, many of which were identified from Streptomyces, a genus that has been well known for its production of bioactive molecules and genetic alterability. Although it was believed that type III PKSs exist exclusively in plants and bacteria, recent fungal genome sequencing projects and biochemical studies revealed the presence of type III PKSs in filamentous fungi, which provides a new chance to study fungal secondary metabolism and synthesize ''unnatural'' natural products. Type III PKSs have been used for the biosynthesis of novel molecules through precursordirected and structure-based mutagenesis approaches.2012 IUBMB IUBMB Life, 64(4): [285][286][287][288][289][290][291][292][293][294][295] 2012

show abstract

Section: Fungal Type III Pkssmentioning

confidence: 99%

Type III polyketide synthases in natural product biosynthesis

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Although type III PKSs are found in bacteria (9) and fungi (5,10), they are more widely distributed in green land plants (Embryophyta). Each plant has a set of taxon-specific type III PKSs, which produce metabolites that are involved in UV protection (flavonoids), antimicrobial defense (stilbenes, bibenzyls, and alkylresorcinols), flower pigmentation (anthocyanins), spore/pollen protection (hydroxyalkyl pyrones), pollen tube growth (flavonols), and legume nodulation (isoflavonoids).…”

mentioning

confidence: 99%

Physcomitrella PpORS, Basal to Plant Type III Polyketide Synthases in Phylogenetic Trees, Is a Very Long Chain 2′-Oxoalkylresorcinol Synthase

Kim

Colpitts

Wiedemann

et al. 2013

Journal of Biological Chemistry

View full text Add to dashboard Cite

Background: Physcomitrella PpORS is an ancient member of the plant type III polyketide synthase (PKS) family. Results: PpORS, produced in nonprotonemal moss cells, synthesizes pentaketide 2Ј-oxoalkylresorcinols using a unique substrate binding site. Conclusion: PpORS is a novel very long chain 2Ј-oxoalkylresorcinol synthase.Significance: This is the first step toward understanding the co-evolution of the type III PKS family and land plants.

show abstract

“…[1,2-13 C 2 ]Acetate feeding experiments showed that csypyrone B1 could be derived from succinyl-CoA and malonylCoAs. 12 Dr Kenji Watanabe (University of Shizuoka) reported the formation of spirocarbon at spirotryprostatin in Saccharomyces cerevisiae by expressing FAD-dependent monooxygenase responsible for fumiquinazoline biosynthesis. Professor Katsuya Gomi (Tohoku University) described the Cre/loxP-mediated marker recycling system.…”

Section: Polyketide and Pksmentioning

confidence: 99%

The International Conference of Natural Product Biosynthesis (ICNPB, 8th US–Japan seminar on the Biosynthesis of Natural Products)

Awakawa

2012

J Antibiot

View full text Add to dashboard Cite

Hideaki Oikawa (Hokkaido University) and the organizing committee, which covered many important topics on biosynthesis of bioactive natural products. Ninety-two participants attended the conference composed of nine sessions. The major topics of this meeting were analyses of biosynthetic routes or enzymes to produce secondary metabolites in microbes and plants, and redesign of the metabolites by engineering the biosynthetic routes or enzymes. This report will briefly summarize some presentations that were given at the conference.

show abstract

Identification of csypyrone B1 as the novel product of Aspergillus oryzae type III polyketide synthase CsyB

Cited by 45 publications

References 22 publications

Type III polyketide synthases in natural product biosynthesis

Type III polyketide synthases in natural product biosynthesis

Physcomitrella PpORS, Basal to Plant Type III Polyketide Synthases in Phylogenetic Trees, Is a Very Long Chain 2′-Oxoalkylresorcinol Synthase

The International Conference of Natural Product Biosynthesis (ICNPB, 8th US–Japan seminar on the Biosynthesis of Natural Products)

Contact Info

Product

Resources

About