Identification of Cryptococcus neoformans by MALDI-TOF mass spectrometry in blood culture

Araújo, Max Roberto Batista; Santos, Elaine Gomes de Morais; Wolf, V.; Seabra, Luisa Ferreira

doi:10.4322/2357-9730.78285

Cited by 1 publication

(2 citation statements)

References 8 publications

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“…The role of the susceptibility test result as a predictor for early or late clinical outcomes remains unclear. A previous study showed some correlation between fluconazole MICs and the poor prognosis of CM [ 4 , 11 , 20 ]. However, we did not observe such correlation in our study.…”

Section: Discussionmentioning

confidence: 99%

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Lack of Association between YEASTONE Antifungal Susceptibility Tests and Clinical Outcomes of Cryptococcus Meningitis

Lin

Cheng

et al. 2023

JoF

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The relation between antifungal susceptibility and treatment outcomes is not well-characterized. There is paucity of surveillance data for cerebrospinal fluid (CSF) isolates of cryptococcus investigated with YEASTONE colorimetric broth microdilution susceptibility testing. A retrospective study of laboratory-confirmed cryptococcus meningitis (CM) patients was conducted. The antifungal susceptibility of CSF isolates was determined using YEASTONE colorimetric broth microdilution. Clinical parameters, CSF laboratory indices, and antifungal susceptibility results were analyzed to identify risk factors for mortality. High rates of resistance to fluconazole and flucytosine were observed in this cohort. Voriconazole had the lowest MIC (0.06 µg/mL) and lowest rate of resistance (3.8%). In a univariate analysis, hematological malignancy, concurrent cryptococcemia, high Sequential Organ Failure Assessment (SOFA) score, low Glasgow coma scale (GCS) score, low CSF glucose level, high CSF cryptococcal antigen titer, and high serum cryptococcal antigen burden were associated with mortality. In a multivariate analysis, meningitis with concurrent cryptococcemia, GCS score, and high CSF cryptococcus burden, were independent predictors of poor prognosis. Both early and late mortality rates were not significantly different between CM wild type and non-wild type species.

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Section: Discussionmentioning

confidence: 99%

“…Cryptococcus species were identified using matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF MS) following the manufacturer’s recommendations [ 10 , 11 , 12 ].…”

Section: Methodsmentioning

confidence: 99%

Lack of Association between YEASTONE Antifungal Susceptibility Tests and Clinical Outcomes of Cryptococcus Meningitis

Lin

Cheng

et al. 2023

JoF

View full text Add to dashboard Cite

show abstract

Identification of Cryptococcus neoformans by MALDI-TOF mass spectrometry in blood culture

Cited by 1 publication

References 8 publications

Lack of Association between YEASTONE Antifungal Susceptibility Tests and Clinical Outcomes of Cryptococcus Meningitis

Lack of Association between YEASTONE Antifungal Susceptibility Tests and Clinical Outcomes of Cryptococcus Meningitis

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