Identification of Cryptic Species of Four Candida Complexes in a Culture Collection

Fontecha, Gustavo; Montes, Kathy; Ortiz, Bryan; Galindo, Celeste; Braham, Sharleen

doi:10.3390/jof5040117

Cited by 24 publications

(37 citation statements)

References 72 publications

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“…NAC species were identified by PCR-RFLP assays using universal primers (Integrated DNA Technologies, USA) and restriction enzyme MspI as described by previous studies [24,25]. Total volume of PCR reactions was 30 μl containing 3 μl of DNA solution, 15 μl 2X Master Mix (Cat.# M7505, Promega, USA), 0.75 μl of each primer (0.25 μM) and distilled water up to 30 μl.…”

Section: Determination Of Nac Based On Pcr-rflp Techniquementioning

confidence: 99%

Prevalence, species distribution and antifungal susceptibility of Candida albicans causing vaginal discharge among symptomatic non-pregnant women of reproductive age at a tertiary care hospital, Vietnam

et al. 2021

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Background Vaginal candidiasis is frequent in women of reproductive age. Accurate identification Candida provides helpful information for successful therapy and epidemiology study; however, there are very limited data from the Vietnam have been reported. This study was performed to determine the prevalence, species distribution of yeast causing vaginal discharge and antifungal susceptibility patterns of Candida albicans among symptomatic non-pregnant women of reproductive age. Methods Vaginal discharge samples were collected from 462 women of reproductive age in Hanoi, Vietnam between Sep 2019 and Oct 2020. Vaginal swabs from these patients were examined by direct microscopic examination (10% KOH). CHROMagar™ Candida medium and Sabouraud dextrose agar supplemented with chloramphenicol (0.5 g/l) were used to isolate yeast, and species identification was performed using morphological tests and molecular tools (PCR and sequencing). Antifungal susceptibility testing was determined according to the Clinical and Laboratory Standards Institute guidelines (M27-A3 and M27-S4). Results The prevalence of vaginal yeast colonization in non-pregnant women was 51.3% of 462 participants. Nine different yeast species were identified. Among these isolates, C. albicans (51.37%) was the most frequent, followed by C. parapsilosis (25.88%), C. glabrata (11.37%), C. tropicalis (4.31%), C. krusei (3.92%), C. africana (1.57%), Saccharomyces cerevisiae (0.78%), C. nivariensis (1 isolates, 0.39%), and C. lusitaniae (1 isolates, 0.39%), respectively. Among C. albicans, all 46 isolates were 100% susceptible to micafungin, caspofungin, and miconazole. The susceptibility rates to amphotericine B, 5-flucytosine, fluconazole, itraconazole and voriconazole were 95.65, 91.30, 91.30, 82.61 and 86.95%, respectively. Conclusions The prevalence of VVC among symptomatic non-pregnant women of reproductive age in Vietnam was higher than many parts of the world. The high frequency of non-albicans Candida species, which were often more resistant to antifungal agents, was a notable feature. Resistance rates of vaginal C. albicans isolates to antifungal agents was low. Our findings suggest that continued surveillance of changes in species distribution and susceptibility to antifungals should be routinely screened and treated.

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Section: Determination Of Nac Based On Pcr-rflp Techniquementioning

confidence: 99%

Prevalence, species distribution and antifungal susceptibility of Candida albicans causing vaginal discharge among symptomatic non-pregnant women of reproductive age at a tertiary care hospital, Vietnam

et al. 2021

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“…Within the isolates classified in the albicans complex, 2.8% were C. dubliniensis , while C. africana was not found. Fontecha et al reported 5% of C. dubliniensis and 3% of C. africana among 66 yeasts isolated in the same hospital [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

“…Since C. dubliniensis was first identified in Ireland as the cause of oropharyngeal candidiasis in patients living with HIV, it has been identified in many other countries [ 19 , 28 , 57 - 61 ]. The pathogenesis and antifungal susceptibility of C. dubliniensis are not clearly defined.…”

Section: Discussionmentioning

confidence: 99%

“…A technique was optimized based on pools in order to offer an easy and economical method to identify C. auris . This method was then applied to screen 112 suspected isolates of C. auris , and as reported in 2017 and 2019, it was not possible to demonstrate the presence of C. auris in the country yet [ 20 , 57 ]. Consequently, the evidence seems to suggest that C. auris is not yet found in Honduras, or at least in the hospital where this study was carried out.…”

Section: Discussionmentioning

confidence: 99%

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Candida species isolated from clinical samples in a tertiary hospital in Honduras: Where is Candida auris?

Ortiz¹,

Aguilar²,

Galindo³

et al. 2022

CMM

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Background and Purpose: Infections by emerging and multiresistant Candida species are becoming more frequent throughout the world. This study aimed to describe Candida species in different wards of a tertiary hospital in Honduras. Materials and Methods: The prevalence of species within the C. albicans complex was estimated using a molecular approach, and C. auris was investigated using a yeast poolbased DNA extraction method. In total, 328 yeast isolates were identified using phenotypic approaches. For the identification of species within the albicans complex, a molecular approach based on the size polymorphisms of the hpw1 gene was used. In addition, a technique was optimized based on DNA extraction in pools for the rapid identification of C. auris. Results: A total of 11 species of Candida were identified in the hospital wards. C. albicans showed the highest number of isolates (52.4%). Within the C. albicans complex, C. albicans sensu stricto was the most common, followed by C. dubliniensis. However, C. auris was not found. Conclusion: Reports on the distribution of Candida species in Honduras are limited; accordingly, the data from this study are of importance for a better understanding of their epidemiology. Moreover, a simple method was offered for the detection of C. auris that could help in its detection in low-resource settings.

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“…However, it is described that the methodologies traditionally used for identifying yeasts, such as conventional phenotypic tests (biochemical tests, chromogenic media), are not able to correctly identify the cryptic species of Candida spp. (Fontecha, et al, 2019). An increase in the number of infections caused by unusual Candida species has been described in recent years (Tsai, et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Candida spp. isolated from the bloodstream in a reference hospital in Brazil: using MALDI-TOF/MS to identify unusual species of Candida

Aguiar

Menezes

Penatti

et al. 2022

RSD

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Candidemia is a health problem with a high mortality rate. The aims of this study were to compare the identification of Candida spp. via traditional methodologies and mass spectrometry (MALDI-TOF/MS) in order to verify the antifungal susceptibility profile of these isolates and analyze the clinical evolution of patients. This was a two-year prospective cohort conducted at the Hospital de Clínicas de Uberlândia (HCU-UFU). Candida spp. were identified via VITEK®-2 and MALDI-TOF/MS. Antifungal susceptibility tests were conducted via VITEK®-2. Clinical-demographic data were assessed from medical records and variables were described as mean, standard deviation, frequency and percentage, and risk factors were evaluated using univariate and multivariate analysis. A total of 113 patients and 126 Candida spp. isolates were studied. C. albicans was most frequent (30%); MALDI-TOF/MS identified unusual species, including misidentification of C. famata. Most of the isolates (95%) were susceptible to the tested antifungals. Most patients were men (65%) and older than 60 years of age (45%); 85% of patients were treated with fluconazole. The 30-day mortality rate was 56%; patients who did not receive treatment and those who started later had a lower survival rate (p <0.0001). MALDI-TOF/MS was able to identify unusual Candida spp. Candidemia presented a high mortality rate; the correct identification of Candida spp. and the introduction of appropriate and timely therapy are essential for increasing patient survival.

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Identification of Cryptic Species of Four Candida Complexes in a Culture Collection

Cited by 24 publications

References 72 publications

Prevalence, species distribution and antifungal susceptibility of Candida albicans causing vaginal discharge among symptomatic non-pregnant women of reproductive age at a tertiary care hospital, Vietnam

Prevalence, species distribution and antifungal susceptibility of Candida albicans causing vaginal discharge among symptomatic non-pregnant women of reproductive age at a tertiary care hospital, Vietnam

Candida species isolated from clinical samples in a tertiary hospital in Honduras: Where is Candida auris?

Characterization of Candida spp. isolated from the bloodstream in a reference hospital in Brazil: using MALDI-TOF/MS to identify unusual species of Candida

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