Identification of Cronobacter spp (enterobacter sakazakii) from raw milk and environmental samples of  dairy farms

Berhilevych, Oleksandra; Касянчук, В. В.

doi:10.15587/1729-4061.2017.114637

Cited by 7 publications

(6 citation statements)

References 14 publications

(26 reference statements)

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“…In the current work, the overall C. sakazakii isolation rate of (6.7 %) is nearly similar to that has been recently reported A potent method for the quick and accurate identification of C. sakazakii and other Cronobacter species is the PCR detection of the 16S rRNA gene. (Bergilevich et al, 2015;Chen et al, 2018). In this study, five isolates were tested with PCR, and all were accurately identified as C. sakazakii.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Molecular identification of Cronobacter sakazakii isolated from different foodstuffs and infant stools

Abuelsoud,

Rizk,

Maarouf

2023

Benha Veterinary Medical Journal

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Cronobacter sakazakii is a foodborne pathogen that can cause serious infections (enterocolitis and meningitis) with high fatalities in neonatal infants. This work was designed to isolate Cronobacter sakazakii and explore its molecular identification and prevalence rate. The present study was performed on 225 random samples of powdered milk infant formula, milk powder, minced meat, fresh cow milk, and diarrheic infant stool (45 for each) collected from different shops and hospitals in Cairo, Kaliobia, and Monofia Governorates, Egypt. Cronobacter sakazakii isolates were characterized by conventional phenotypical methods and MICROBACT TM kits, then genotypic identification of the common 16S rRNA gene by PCR. Out of the fifteen Cronobacter sakazakii isolates obtained, the majority were isolated from powdered milk infant formula samples 5 isolates (11.1%), 4 from milk powder (8.9%), 3 from diarrheic infant stool (6.7%), 2 from minced meat (4.4%), and 1 from fresh cow milk samples (2.2%). The Cronobacter sakazakii 16S rRNA gene was detected in all tested isolates. Therefore, the results of this work highlight the importance of Cronobacter sakazakii as a high public health concern in newborns, indicating the potential risk of infant infection by this bacteria from mostly all sources of infant food.

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Section: Discussionmentioning

confidence: 99%

Isolation and Molecular identification of Cronobacter sakazakii isolated from different foodstuffs and infant stools

Abuelsoud,

Rizk,

Maarouf

2023

Benha Veterinary Medical Journal

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“…To this end, the samples of raw milk with different content of fat, protein, and titrated acidity were stored under the following conditions: the temperature is 4, 6, 8, and 10 °C, the duration is from 2 to 48 hours. Each sample of milk was examined after 1,2,4,6,8,10,12,16,20,24,36, and 48 hours. The influence of milk acidity on the number of bacteria from the Enterobacteriaceae family was determined for the following values: 16,17,18,19,20 °T.…”

Section: Discussion Of Studying the Efficiency Of A Predicting Technimentioning

confidence: 99%

“…The microbial contamination of raw milk may happen from three main sources: from the udder, from the surface of the skin of the udder, and from dairy equipment. The lack of sanitary-and-hygienic conditions during milking increases the amount of bacteria in raw milk [9][10][11]. Enterobacteriaceae is a family of the gram-negative, non-sporous bacteria, which ferment lactose with the formation of acid and gas.…”

Section: в даний час встановлено що штучні нейронні мережі (шнм) забmentioning

confidence: 99%

“…The models involved the samples of raw milk with a different content of fat, protein, and acidity, stored at different parameters for temperature and duration. Each sample of milk was examined after 1,2,4,6,8,10,12,16,20,24,36 The design of new ANNs was used to establish the relationship between the input and output layers. The network consists of three layers: an input layer (5 input parameters: milk storage temperature (4, 6, 8, and 10 °C), duration of milk storage (from 2 to 48 hours), the content of fat and protein in milk (%), the acidity of milk (°T), the hidden layers (with 30 neurons), and an output layer (the predicted number of bacteria from the Enterobacteriaceae family), as shown in Fig.…”

Section: Design Of Experimental Models and Building A Database On mentioning

confidence: 99%

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Construction of a method for predicting the number of enterobacteria in milk using artifical neural networks

Berhilevych

Касянчук

Chernetskyi

et al. 2019

EEJET

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“…Cronobacter sakazakii is ubiquitous and has been isolated from a wide variety of sources including dried herbs and spices, soil, starches, milk products, and expressed breast milk ( Berhilevych and Kasianchuk, 2017 ; Forsythe, 2018 ; Jang et al, 2018a ). However, C. sakazakii can be found in PIF, factories, hospitals, and homes ( Centre for Disease Control, 2020b ).…”

Section: Cronobacter Speciesmentioning

confidence: 99%

Current and Future Perspectives on the Role of Probiotics, Prebiotics, and Synbiotics in Controlling Pathogenic Cronobacter Spp. in Infants

Parreira

Goodridge

et al. 2021

Front. Microbiol.

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Cronobacter species, in particular C. sakazakii, is an opportunistic bacterial pathogen implicated in the development of potentially debilitating illnesses in infants (<12months old). The combination of a poorly developed immune system and gut microbiota put infants at a higher risk of infection compared to other age groups. Probiotics and prebiotics are incorporated in powdered infant formula and, in addition to strengthening gut physiology and stimulating the growth of commensal gut microbiota, have proven antimicrobial capabilities. Postbiotics in the cell-free supernatant of a microbial culture are derived from probiotics and can also exert health benefits. Synbiotics, a mixture of probiotics and prebiotics, may provide further advantages as probiotics and gut commensals degrade prebiotics into short-chain fatty acids that can provide benefits to the host. Cell-culture and animal models have been widely used to study foodborne pathogens, but sophisticated gut models have been recently developed to better mimic the gut conditions, thus giving a more accurate representation of how various treatments can affect the survival and pathogenicity of foodborne pathogens. This review aims to summarize the current understanding on the connection between Cronobacter infections and infants, as well as highlight the potential efficacy of probiotics, prebiotics, and synbiotics in reducing invasive Cronobacter infections during early infancy.

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Identification of Cronobacter spp (enterobacter sakazakii) from raw milk and environmental samples of dairy farms

Cited by 7 publications

References 14 publications

Isolation and Molecular identification of Cronobacter sakazakii isolated from different foodstuffs and infant stools

Isolation and Molecular identification of Cronobacter sakazakii isolated from different foodstuffs and infant stools

Construction of a method for predicting the number of enterobacteria in milk using artifical neural networks

Current and Future Perspectives on the Role of Probiotics, Prebiotics, and Synbiotics in Controlling Pathogenic Cronobacter Spp. in Infants

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