Identification of CRMP4 as a Convergent Regulator of Axon Outgrowth Inhibition

Alabed, Yazan Z.; Pool, Madeline; Tone, Stephan Ong; Fournier, Alyson E.

doi:10.1523/jneurosci.5055-06.2007

Cited by 96 publications

(142 citation statements)

References 58 publications

(75 reference statements)

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“…28), mouse polyclonal DPYSL3 (1:1000; Abnova, Taiwan), mouse ␤III-tubulin (1:1000 dilution; Covance, Princeton, NJ), mouse mCherry (1:200; Abcam, La Jolla, CA), mouse actin (1:2000; Millipore, Bedford, MA), mouse GAPDH (1:1000; Abcam), Alexa Fluor secondary antibodies (1:1000 dilution; Invitrogen), and HRP-coupled IgG antibodies (1:10,000; Jackson ImmunoResearch Laboratories, West Grove, PA). F-actin was labeled with rhodamine-or fluorescein-labeled phalloidin (1:250; Invitrogen).…”

Section: Methodsmentioning

confidence: 99%

“…Constructs-The HSV-CRMP4S-V5 construct was described previously (28). CRMP4S, CRMP4⌬N300 (amino acids 300 -570), CRMP4⌬C50 (amino acids 1-520), CRMP4⌬C100 (amino acids 1-470), and CRMP4⌬470 -520 (deletion of amino acids 470 -520) DNAs were amplified by PCR from the pcDNA3.1/V5-His-CRMP4S construct and subcloned into the pHSV-PUC-IRES-GFP vector using SalI and XbaI restriction sites.…”

Section: Methodsmentioning

confidence: 99%

“…Distribution of CRMP4 in Hippocampal Neurons-To investigate the potential mechanism through which CRMP4 impacts growth cone morphology and axon growth, we stained 3 DIV hippocampal neurons with an anti-CRMP4 antibody that recognizes both short and long CRMP4 isoforms (28). CRMP4L was weakly expressed in lysates from E18 hippocampal neurons; thus our staining likely reflects the expression of CRMP4S (Fig.…”

Section: Crmp4 Promotes Growth Cone Spreading and Axonmentioning

confidence: 99%

“…In addition, the CRMP4 isoform binds directly to F-actin and organizes F-actin into bundles in vitro. CRMP4 is also the sole CRMP isoform that can bind to RhoA, a key regulator of the actin cytoskeleton (27,28). In zebrafish, CRMP4 function is important for proper positioning of neural crest cells and caudal primary motor neurons, whereas in the mouse, CRMP4 supports proper bifurcation of apical dendrites (29 -31).…”

mentioning

confidence: 99%

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Collapsin Response Mediator Protein 4 Regulates Growth Cone Dynamics through the Actin and Microtubule Cytoskeleton

Khazaei

Girouard

Alchini

et al. 2014

Journal of Biological Chemistry

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Background: Intricate regulation of the growth cone cytoskeleton controls growth cone dynamics. Results: Loss of CRMP4 disrupts growth cone cytoskeletal dynamics, growth cone expansion, and axon growth. Conclusion: CRMP4 regulates both the actin and microtubule growth cone cytoskeleton. Significance: CRMP4 plays a critical role in regulating cytoskeletal dynamics underlying growth cone properties.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Crmp4 Promotes Growth Cone Spreading and Axonmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Collapsin Response Mediator Protein 4 Regulates Growth Cone Dynamics through the Actin and Microtubule Cytoskeleton

Khazaei

Girouard

Alchini

et al. 2014

Journal of Biological Chemistry

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“…Additionally, seven genes were identified that were categorized by the gene ontology biological process GO:0007399 (nervous system development) (P Ͻ 0.04). Three of these genes encode components of signal transduction pathways that regulate the movement and/or development of neurons within the embryonic brain: chemokine (C-X-C motif) receptor 4 (Cxcr4), glypican 4 (Gpc4), and dihydropyrimidinaselike 4 (Dpysl4) (30)(31)(32)(33)(34)(35). For example, Cxcr4 regulates the migration of embryonic neurons into the cerebellum (35).…”

Section: Stage-dependent Expression By Sertoli Cells Of Genes Previouslymentioning

confidence: 99%

Stage-specific gene expression is a fundamental characteristic of rat spermatogenic cells and Sertoli cells

Johnston¹,

Wright

DiCandeloro³

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

135

141

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Mammalian spermatogenesis is a complex biological process that occurs within a highly organized tissue, the seminiferous epithelium. The coordinated maturation of spermatogonia, spermatocytes, and spermatids suggests the existence of precise programs of gene expression in these cells and in their neighboring somatic Sertoli cells. The objective of this study was to identify the genes that execute these programs. Rat seminiferous tubules at stages I, II-III, IV-V, VI, VIIa,b, VIIc,d, VIII, IX-XI, XII, and XIII-XIV of the cycle were isolated by microdissection, whereas Sertoli cells, spermatogonia plus early spermatocytes, pachytene spermatocytes, and round spermatids were purified from enzymatically dispersed testes. Microarray analysis by using Rat Genome 230 2.0 arrays identified 16,971 probe sets that recognized testicular transcripts, and 398 of these were identified as testis-specific. Expression of 1,286 probe sets were found to differ at least 4-fold between two cell types and also across the stages of the cycle. Pathway and annotated cluster analyses of those probe sets predicted that entire biological pathways and processes are regulated cyclically in specific cells. Important among these are the cell cycle, DNA repair, and embryonic neuron development. Taken together, these data indicate that stage-regulated gene expression is a widespread and fundamental characteristic of spermatogenic cells and Sertoli cells.array analysis ͉ spermatogenesis ͉ seminiferous tubules ͉ stages of the cycle of the seminiferous epithellum ͉ contraception I n mammals, spermatogenesis encompasses a series of precisely timed cellular events that take place in a highly organized tissue, the seminiferous epithelium. Within each cross-section of this tissue, spermatogonia, spermatocytes, and spermatids are in intimate physical association with somatic Sertoli cells, and, together, these cells progress synchronously through the stages of the cycle of the seminiferous epithelium (1, 2). Four cycles are required for spermatogonia, and their progeny to complete spermatogenesis. The synchrony of germ cell development has a significant effect on the structures and functions of both the germ cells and their associated somatic Sertoli cells, suggesting the existence of precise and coordinated cyclic programs of gene expression. However, because investigators have evaluated the stage-dependent changes in expression of only a few genes, whether or not cyclic gene expression is a rare or prevalent characteristic of these cells is unknown (3-7). It is also not known whether genes encoding components of important biological pathways and processes are coordinately regulated in a cyclic manner. However, such genes may represent new targets for the development of male contraceptives.Given the coordinated nature of spermatogenesis, we hypothesized that the expression of large numbers of genes differ substantially, both between specific cell types within the seminiferous epithelium and within a given cell type as it progresses through the stages of...

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Post‐translational regulation of Crmp in developing and regenerating chick spinal cord

Gögel¹,

Lange²,

Leung³

et al. 2010

Developmental Neurobiology

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It is becoming apparent that regulation at the protein level plays crucial roles in developmental and pathological processes. Therefore, we performed a proteomics screen to identify proteins that are differently expressed or modified at stages of development permissive (E11) and nonpermissive for regeneration (E15) of the chick spinal cord. Proteins regulated either developmentally or in response to spinal-cord injury included collapsin-response-mediator proteins (Crmps), known to modulate microtubule dynamic and axonal growth. No significant changes in Crmp transcripts following injury were observed, indicating regulation mainly at the protein level. Analysis of Crmp-2 protein and its phosphorylated forms, pS522 and pT514, showed that Crmp-2 is developmentally regulated and also expressed in neural progenitors in vivo and in neurospheres. Its cellular localization changed both with development and following spinal-cord injury. In addition, although overall levels of Crmp-2 expression were not affected by injury, abundance of certain phosphorylated forms was altered. pT514 Crmp-2 appeared to be associated with dividing neural progenitors and was greatly reduced at nonpermissive stages for regeneration, whereas it did not seem affected by injury. In contrast, phosphorylation of Crmp-2 at S522 was upregulated early after injury in regenerating spinal cords and the ratio between phosphorylated to total Crmp-2 increased, as indicated by 2D Western blots. Altogether, this study shows highly dynamic regulation of Crmp-2 forms during development and identifies post-translational changes in Crmp-2 as putative contributors to the maintenance of spinal-cord regenerative ability, possibly via a transient stabilization of the neuronal cytoskeleton.

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Identification of CRMP4 as a Convergent Regulator of Axon Outgrowth Inhibition

Cited by 96 publications

References 58 publications

Collapsin Response Mediator Protein 4 Regulates Growth Cone Dynamics through the Actin and Microtubule Cytoskeleton

Collapsin Response Mediator Protein 4 Regulates Growth Cone Dynamics through the Actin and Microtubule Cytoskeleton

Stage-specific gene expression is a fundamental characteristic of rat spermatogenic cells and Sertoli cells

Post‐translational regulation of Crmp in developing and regenerating chick spinal cord

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