Identification of critically carcinogenesis-related genes in basal cell carcinoma

Abstract

BackgroundBasal cell carcinoma (BCC) is a frequent malignant tumor of skin cancers with high morbidity. The objective of this study was to identify critical genes and pathways related to the carcinogenesis of BCC and gain more insights into the underlying molecular mechanisms of BCC.Materials and methodsThe gene expression profiles of GSE7553 and GSE103439 were downloaded from the Gene Expression Omnibus database with 19 tumors and 6 normal skin tissues. Differentially expressed genes (DEGs) were screened between BCC samples and normal tissues, followed by gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis. Subsequently, protein–protein interaction (PPI) network was constructed for these DEGs, and module analysis was performed.ResultsA total of 313 DEGs were obtained. Among them, 222 genes were upregulated and 91 genes were downregulated. Enrichment analysis indicated that the upregulated genes were significantly enriched in cell cycle and mitosis, while the downregulated genes were mainly associated with unsaturated fatty acid metabolic process and cell differentiation. In addition, TOP2A, CDK1, and CCNB1 were identified as the top three hub genes ranked by degrees in the PPI network. Meanwhile, three subnetworks were derived, which indicated that these DEGs were significantly enriched in pathways, including “cell cycle”, “extracellular matrix–receptor interaction”, “basal cell carcinoma”, and “hedgehog signaling pathway”.ConclusionsThe novel critical DEGs and pathways identified in this study may serve pivotal roles in the carcinogenesis of BCC and indicate more molecular targets for the treatment of BCC.

Genome-wide promoter methylation profiling in a cellular model of melanoma progression reveals markers of malignancy and metastasis that predict melanoma survival

“…This is the first time that the promoter methylation levels of Pyrodx2 and Ptgfrn have been described in melanoma, which could be further explored as novel individual biomarkers for this disease. PYROXD2 is differentially expressed in basal cell carcinoma [ 47 ] and is localized in the mitochondrial inner membrane, where it is associated with increased cell proliferation and ATP production [ 48 ], highlighting its role in melanoma survival and metabolism.…”

Clinicopathological and dermoscopic correlation of pigmented basal cell carcinoma

“…It is more common in males. 3 The main etiopathogenensis is due to ultraviolet radiation which causes mutations in p53 tumour suppressor gene and damages DNA. Sonic hedgehog signalling pathway is activated by loss of PTCH 1 which activates smoothened gene (SMO) thus inhibiting suppressor fused (SUFU) gene leading to activation of GL1, 2, 3.…”

Towards Improving Skin Cancer Diagnosis by Integrating Microarray and RNA-Seq Datasets

“…On the one hand, the most remarkable inquiries underlined the dysregulation of up to 6 DEGs in MSC cases [83], [84] and development risk [85] (except ADAMTS3 and PI3) and the implication of up to 5 DEGs in PS development or inflammatory processes [80], [86] (except MLANA, ADAMTS3 and KRT14). On the other hand, the differentiating role of specific DEGs in NMSC cases was highlighted: the overexpression of ADAMTS3 in BCC [87] or the hypothetical implication of KRT14 in the malignant transformation of potential stem cells as origin of MCC [88]. Based on all these precedent evidences and the results shown (Fig.…”