Identification of cortactin molecular forms in human urine and their possible diagnostic value

Starykovych, M. O.; Souchelnytskyi, Serhiy; Fayura, O.; Abrahamovych, Orest; Abrahamovych, M.; Lukavetskyy, N.; Stoika, Rostyslav; Kit, Yu. Ya.

doi:10.15407/ubj93.04.103

Cited by 1 publication

(1 citation statement)

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“…The protein content of urine samples taken from patients with liver cirrhosis or lung cancer and from healthy donors was investigated using a precipitation procedure followed by SDS electrophoresis and MALDI analysis of the relevant excised and digested bands [ 62 ]. Peptide mass fingerprinting identified a 100 kDa protein as the Src substrate cortactin, while various molecular forms of cortactin that differ in the tested groups were identified by Western blot analysis with specific anti-human cortactin antibodies, implying that urine cortactin isoforms could be measured for the diagnosis of acute and systemic diseases.…”

Section: Urine Samples Analysismentioning

confidence: 99%

MALDI-TOF/MS Analysis of Non-Invasive Human Urine and Saliva Samples for the Identification of New Cancer Biomarkers

Zambonin

Aresta

2022

Molecules

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Cancer represents a group of heterogeneous diseases that are a leading global cause of death. Even though mortality has decreased in the past thirty years for different reasons, most patients are still diagnosed at the advanced stage, with limited therapeutic choices and poor outcomes. Moreover, the majority of cancers are detected using invasive painful methods, such as endoscopic biopsy, making the development of non-invasive or minimally invasive methods for the discovery and fast detection of specific biomarkers a crucial need. Among body fluids, a valuable non-invasive alternative to tissue biopsy, the most accessible and least invasive are undoubtedly urine and saliva. They are easily retrievable complex fluids containing a large variety of endogenous compounds that may provide information on the physiological condition of the body. The combined analysis of these fluids with matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF/MS), a reliable and easy-to-use instrumentation that provides information with relatively simple sample pretreatments, could represent the ideal option to rapidly achieve fast early stage diagnosis of tumors and their real-time monitoring. On this basis, the present review summarizes the recently reported applications relevant to the MALDI analysis of human urine and saliva samples.

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Section: Urine Samples Analysismentioning

confidence: 99%