Identification of consumed stone flounder, Kareius bicoloratus (Basilewsky), from the stomach contents of sand shrimp, Crangon affinis (De Haan) using mitochondrial DNA analysis

Asahida, Takashi; Yamashita, Yoh; Kobayashi, Takanori

doi:10.1016/s0022-0981(97)00039-7

Cited by 57 publications

(40 citation statements)

References 10 publications

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“…Although piscivorous predation is largely absent from this region, invertebrate predation may be significant. Although the common invertebrate marine predators such as chaetognaths, cnidarians and ctenophores are absent from the ETM brackish water, many other potential predators are found in high densities, for example, the sand shrimp Crangon septemspinosa, a documented predator of fish larvae (Bertram & Leggett 1994, Witting & Able 1995, Asahida et al 1997. Investigations of predation must be carried out before we can conclude that predation contributes to the sizeselective mortality of rainbow smelt larvae documented here.…”

Section: Impact On Vertical Migrationmentioning

confidence: 87%

Cestode parasitism as a regulator of early life-history survival in an estuarine population of rainbow smelt Osmerus mordax

Bourque¹,

Dodson

Ryan³

et al. 2006

Mar. Ecol. Prog. Ser.

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In the St. Lawrence middle estuary, the larvae and juveniles of rainbow smelt Osmerus mordax are infected with intestinal parasites (the cestode Proteocephalus tetrastomus). Such parasitism is suspected to impair growth rate, which may in turn influence the ability of smelt to migrate vertically and to be retained in the estuarine turbidity maximum (ETM), the nursery area of this population. We hypothesised that parasitism contributes to size-selective mortality by altering patterns of vertical migration, thus contributing to transport losses from the nursery area. Parasitism was found to affect growth rate, as determined by otolith microstructure analysis, and to contribute to mortality. Larvae which survived the parasitic infection were found to be, on average, larger than the uninfected survivors at some point in their early life history prior to infection. Significant sizeselective mortality was observed among infected larvae relative to uninfected larvae. There was, however, no significant difference in the vertical distribution of parasitised versus non-parasitised larvae, indicating that size-selective mortality was not due to transport losses from the nursery area. Rather, starvation or predation was most likely responsible for observed mortality patterns. We illustrate that variability in the incidence of parasitic infection among years may form the basis for variability in recruitment and the abundance of the adult smelt population.

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Section: Impact On Vertical Migrationmentioning

confidence: 87%

Cestode parasitism as a regulator of early life-history survival in an estuarine population of rainbow smelt Osmerus mordax

Bourque¹,

Dodson

Ryan³

et al. 2006

Mar. Ecol. Prog. Ser.

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“…Numerous studies focused on the polymerase chain reaction (PCR) technology, which can reproduce millions of copies in vitro. Traditionally, the next step depends on various means to discriminate the species using species-specific DNA sequences, such as RAPD (Agustí et al 1999), RFLP (Asahida et al 1997), Group-specific primer (Symondson et al 2002;Admassu et al 2006) or multiple PCR (Harwood et al 2007). Moreover, quantitative polymerase chain reaction (qPCR) is a powerful tool for trophic linkages analysis (Zhang et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Application of a novel method PCR-ligase detection reaction for tracking predator—prey trophic links in insect-resistant GM rice ecosystem

Tian

Wang

et al. 2011

Ecotoxicology

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Insect-resistant genetically modified (IRGM) rice is on the verge of commercial release in China, however, its potential non-target effect on non-target insect natural enemies remains controversial. Tracking trophic interactions between predators and preys in IRGM rice ecosystem can provide new insights into better understanding of the ecological risks of IRGM rice. In the present study, a novel method based on ligase detection reaction (LDR), PCR-LDR was introduced to track 15 prey species in the gut of a predaceous spider Pirata subpiraticus, a dominant natural enemy in rice field. Our results indicated that PCR-LDR could provide high specificity and sensitivity in tracking prey-predator interactions in rice ecosystems. PCR-LDR could detect as little as 1,000 th of DNA mixture. Reliable detection of DNA samples of prey species using PCR-LDR could be significantly affected by digestion time and prey species. In the analysis of 200 field-collected P. subpiraticus and 105 field-collected Tetragnatha maxillosa individuals using PCR-LDR, prey remains were identified in 78.3 and 74.3% of the individuals, respectively, from which significant predation differences between the two spider species were observed. Predation behavior of the spider species was not significantly different between Bt and non-Bt control rice lines. These results indicated that PCR-LDR can be used as an important tool for ecological studies, especially on the interactions between predators and preys in IRGM rice or other similar ecosystems.

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“…Once the primers have been designed, they can easily and inexpensively be applied in other studies. Although this approach has been successfully applied in marine (Asahida et al 1997;Jarman et al 2002) and terrestrial predator-prey systems (Agusti et al 2003a), the molecular toolbox has to be improved to study complex food webs.…”

Section: Introductionmentioning

confidence: 99%

Detecting predation and scavenging by DNA gut-content analysis: a case study using a soil insect predator-prey system

2004

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White grubs (larvae of Coleoptera: Scarabaeidae) are abundant in below-ground systems and can cause considerable damage to a wide variety of crops by feeding on roots. White grub populations may be controlled by natural enemies, but the predator guild of the European species is barely known. Trophic interactions within soil food webs are difficult to study with conventional methods. Therefore, a polymerase chain reaction (PCR)-based approach was developed to investigate, for the first time, a soil insect predator-prey system. Can, however, highly sensitive detection methods identify carrion prey in predators, as has been shown for fresh prey? Fresh Melolontha melolontha (L.) larvae and 1- to 9-day-old carcasses were presented to Poecilus versicolor Sturm larvae. Mitochondrial cytochrome oxidase subunit I fragments of the prey, 175, 327 and 387 bp long, were detectable in 50% of the predators 32 h after feeding. Detectability decreased to 18% when a 585 bp sequence was amplified. Meal size and digestion capacity of individual predators had no influence on prey detection. Although prey consumption was negatively correlated with cadaver age, carrion prey could be detected by PCR as efficiently as fresh prey irrespective of carrion age. This is the first proof that PCR-based techniques are highly efficient and sensitive, both in fresh and carrion prey detection. Thus, if active predation has to be distinguished from scavenging, then additional approaches are needed to interpret the picture of prey choice derived by highly sensitive detection methods.

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Identification of consumed stone flounder, Kareius bicoloratus (Basilewsky), from the stomach contents of sand shrimp, Crangon affinis (De Haan) using mitochondrial DNA analysis

Cited by 57 publications

References 10 publications

Cestode parasitism as a regulator of early life-history survival in an estuarine population of rainbow smelt Osmerus mordax

Cestode parasitism as a regulator of early life-history survival in an estuarine population of rainbow smelt Osmerus mordax

Application of a novel method PCR-ligase detection reaction for tracking predator—prey trophic links in insect-resistant GM rice ecosystem

Detecting predation and scavenging by DNA gut-content analysis: a case study using a soil insect predator-prey system

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