Identification of Coniferin-Specific-Glucosidases in Cicer arietinum L. Seedlings

Arabidopsis thaliana β-Glucosidases BGLU45 and BGLU46 hydrolyse monolignol glucosides

“…Table 3 also shows that, among chromogenic substrates tested, oNPGlc was most rapidly hydrolysed by BGLU45 and BGLU46 with relative rates of 619% and 449%, respectively, of the rate of pNPGlc degradation. pNPGlc was also a substrate for other monolignol glucoside b-glucosidases, and, where tested, oNPGlc was hydrolysed 2-7-fold faster than pNPGlc (Marcinowski and Grisebach, 1978;Surholt and Hö sel, 1981;Leinhos et al, 1994;Dharmawardhana et al, 1995).…”

“…No activity was detectable toward any other natural substrate tested. High activity toward syringin and coniferin with relatively poor utilization of CAG is a hallmark of known monolignol glucoside b-glucosidases (Marcinowski and Grisebach, 1978;Hö sel et al, 1978;Surholt and Hö sel, 1981;Hö sel and Todenhagen, 1980;Dharmawardhana et al, 1995). Furthermore, this substrate preference reflects the composition of dicotyledonous angiosperm lignins, which consist principally of S and G units (derived from sinapyl and coniferyl alcohols, respectively) with traces of H units (derived from p-coumaryl alcohol) .…”

Relationship of coniferin β-glucosidase to lignification in various plant cell suspension cultures

“…However, further experiments to prove this hypothesis were not performed, and the hypothesis was questioned by others [8,12,27]. Recently,/3-glucosidases with high specificity for the possible lJgnin precursors coniferin and syringin have been identified from spruce (Picea abies) seedlings [21 ], chickpea (Cicer arietinum L.) [14,29], and soybean (Glycine max.) [13] seedlings and cell cultures.…”

Rational design, synthesis and biological evaluation of the first inhibitor of lignin polymerization

“…18 Glucosides 3 are the natural protected forms of cinnamyl alcohols 1 and have been extensively used for radiolabelling experiments, 19,20 the glucosyl residue being cleaved in situ by a b-glucosidase before the polymerization step. [21][22][23] b-Fluoroconiferin 4 was thus designed as the stable protected form of fluoroconiferyl alcohol, the actual inhibitor.…”