Identification of common dermatophytes (Trichophyton,Microsporum,Epidermophyton) using polymerase chain reactions

Graser,; Fari, EL; Presber,; Sterry,; Tietz,

doi:10.1046/j.1365-2133.1998.02165.x

Cited by 98 publications

(86 citation statements)

References 24 publications

(29 reference statements)

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“…1). Our results fitted well with the known predilection of dermatophyte species for certain body areas (De Hoog et al, 1998;Fumeaux et al, 2004;Graser et al, 1998Graser et al, , 1999aKardjeva et al, 2006;Ninet et al, 2003;Summerbell et al, 2002;Weitzman & Summerbell, 1995). Clade I human isolates Table 1) were isolated from tinea pedis, like the neotype of T.…”

Section: T T G a C T G T G T G T T T T A T A C A A T G G G A -G T Isupporting

confidence: 74%

Phylogenetic analysis of Trichophyton mentagrophytes human and animal isolates based on MnSOD and ITS sequence comparison

et al. 2007

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Dermatophytes are keratinophilic fungi able to infect keratinized tissues of human or animal origin. Among them, Trichophyton mentagrophytes is known to be a species complex composed of several species or variants, which occur in both human and animals. Since the T. mentagrophytes complex includes both anthropophilic and zoophilic pathogens, accurate molecular identification is a critical issue for comprehensive understanding of the clinical and epidemiological implications of the genetic heterogeneity of this complex. Here, 41 T. mentagrophytes isolates from either human patients (14 isolates) or animals (27 isolates) with dermatophytosis were prospectively isolated by culture and identified on morphological bases at the University Hospital Centres of Lille and Poitiers, and the Veterinary School of Alfort, respectively. The isolates were differentiated by DNA sequencing of the variable internal transcribed spacer (ITS) regions flanking the 5.8S rDNA, and of the housekeeping gene encoding the manganese-containing superoxide dismutase (MnSOD), an enzyme which is involved in defence against oxidative stress and has previously provided interesting insight into both fungal taxonomy and phylogeny. ITS1-ITS2 regions and MnSOD sequences successfully differentiate between members of the T. mentagrophytes complex and the related species Trichophyton rubrum. Whatever the phylogenetic marker used, members of this complex were classified into two major clades exhibiting a similar topology, with a higher variability when the ITS marker was used. Relationships between ITS/MnSOD sequences and host origin, clinical pattern and phenotypic characteristics (macroscopic and microscopic morphologies) were analysed.

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Section: T T G a C T G T G T G T T T T A T A C A A T G G G A -G T Isupporting

confidence: 74%

Phylogenetic analysis of Trichophyton mentagrophytes human and animal isolates based on MnSOD and ITS sequence comparison

et al. 2007

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“…The conventional methods to identify dermatophytes rely on the expression of characteristic phenotypic characters. Identification is often delayed or problematic because isolates may be slow to form conidia or produce atypical microscopic structures or colony appearances that require the assistance of an experienced mycologist who specializes in dermatophytes 24 . Molecular techniques are more beneficial for dermatophyte identification as they are objective, unequivocal, rapid and more sensitive.…”

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confidence: 99%

Correlation Between Phenotypic and Genotypic Identification of Trichophyton Violaceum

Soliman

Ibrahim

Taha

et al. 2014

Zagazig University Medical Journal

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Background: Trichophyton violaceum is an anthropophilic dermatophyte. It is the most common etiologic agent causing dermatophytosis especially tinea capitis in Egypt. As routine phenotypic identification of dermatophytes is either slow or lack specificity, improved identification methods are required. The application of PCR has made rapid and precise identification of T. violaceum possible. Aim: This study was designed to correlate between phenotypic and genotypic identification of T. violaceum. Subjects: Two hundred patients with tinea capitis (122 males and 78 females) with a mean age of 8.5±3.1 were the subjects of this study. Methods: Phenotypic identification of T. violaceum determined by observation of its macroscopic and microscopic characteristics and culture on differential media was correlated with ITS-based PCR. Results: Genotypic identification results of T. violaceum in comparison with phenotypic identification results were identical. Conclusion: Although there is no difference between the results of phenotypic and genotypic identification methods of T. violaceum isolates, genotypic method is recommended as it is rapid and accurate.

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“…Due to some limitations in traditional methods such as the high degree of phenoltypic similarity between these relative species identification and time-consumption, many isolates reveal unusual characteristics (Weiyzman et al, 1995;Graser et al, 2008;Shehata et al, 2008;De Baere et al, 2010). To overcome these problems, in recent years, molecular marker approaches relying on genetic makeup are regarded as useful in the exact and rapid recognition of dermatophytes Kanbe et al, 2003;Liu et al, 2000;Graser et al, 1998). During the present work, three molecular marker systems based on PCR were applied to study the genetic relationships among 18 selected isolates of dermatophytes (12 isolates) and yeasts (6 isolates).…”

Section: Discussionmentioning

confidence: 99%

Genetic relationships and isozyme profile of dermatophytes and Candida strains from Egypt and Libya

Abdel-Fatah¹,

Moharram²,

Moubasher³

et al. 2013

Afr. J. Biotechnol.

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Three molecular techniques random amplified polymorphic DNA (RAPD), inter-simple sequence repeat (ISSR) and restriction fragment length polymorphism (RFLP) were employed for identification and to study the genetic relationship among six species of dermatophytes and three species of yeasts isolated from Egyptian and Libyan patients with skin mycosis. Each species was represented by two isolates, one from Egyptian patients and the second from Libyan. RAPD in which four random 10-mer primers and two ISSR primers were used to amplify the DNA fragments of target fungi and RFLP in which two universal primers (ITS1 and ITS4) were used to amplify the internal transcribed spacer (ITS) regions of the ribosomal (rRNA) gene in fungal isolates followed by digestion with HinfI and HaeIII endonucleases was carried out. Three molecular marker techniques showed considerable potential for identifying and discriminating dermatophytes and Candida species and the achieved results confirmed identification based on conventional morphological methods. Results of RAPD and ISSR markers revealed 78.7% genetic similarity (GS) between Microsporum canis and other tested fungi reflecting a relatively longer genetic distance from other isolates of dermatophytes and yeasts. Candida krusei and Candida tropicalis were closely related showing 93.3% GS. C. albicans showed 90.9% similarity with other species of Candida. Epidermophyton floccosum was easily separated from all Trichophyton species showing 87.3% similarity. Unique bands were displayed by certain fungi and can be taken as a positive marker for isolate identification and discrimination. RFLP technique revealed differences in the number (1 to 5) and size (8 to 378 base pairs) of DNA fragments depending on the fungal isolate and restriction enzyme used. Within each fungal species, different isolates of dermatophytes and Candida from Egypt and Libya showed close relationship. Seven isozyme systems namely esterase, peroxidase, malate dehydrogenase, acid phosphatase, glutamate-oxalo-acetate transaminase, Urease and protease were studied to detect the gene expression and genetic variability among the different isolates of dermatophytes and Candida.

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Identification of common dermatophytes (Trichophyton,Microsporum,Epidermophyton) using polymerase chain reactions

Cited by 98 publications

References 24 publications

Phylogenetic analysis of Trichophyton mentagrophytes human and animal isolates based on MnSOD and ITS sequence comparison

Phylogenetic analysis of Trichophyton mentagrophytes human and animal isolates based on MnSOD and ITS sequence comparison

Correlation Between Phenotypic and Genotypic Identification of Trichophyton Violaceum

Genetic relationships and isozyme profile of dermatophytes and Candida strains from Egypt and Libya

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