Identification of commercial hake and grenadier species by proteomic analysis of the parvalbumin fraction

Abstract

Analysis of parvalbumin fractions through proteomic methodologies allowed the differential classification of ten commercial, closely related species of the family Merlucciidae. Muscle extracts from nine hake species of the genus Merluccius including two subspecies of Merluccius australis (australis and polylepsis) and one grenadier species Macruronus novaezelandiae with two populations (novaezelandiae and magellanicus) were evaluated by 2-DE and MALDI-TOF MS. 2-DE demonstrated that the species tested displayed a low intra-specific degree of polymorphism and the isoform patterns were noticeably species-specific. MALDI-TOF mass fingerprints showed clear differences in the pattern of peptides produced by tryptic digestion between the Merluccius and the Macruronus, making the genus differentiation possible. In addition, a selective peptide mass present in the spectra from certain hakes allowed its classification in two groups: Euro-African and American hakes. Besides, some specific masses allowed a clearly individual identification for M. bilinearis, M. australis polylepsis, M. australis australis, M. productus, M. paradoxus and M. polli, while the rest of the hake species can be grouped in two clusters, comprising M. hubbsi and M. gayi in one and M. merluccius and M. capensis in the other.

Fish Allergen Detection

“…Analytical methods for the detection of fish in foods are necessary to monitor hygiene practices and to ensure compliance with legislation (Poms et al, 2004). A number of different techniques for the identification of parvalbumin by chromatography, isoelectric focussing, or mass spectrometry have been developed (Ross et al, 1998;Etienne et al, 2000;Carrera et al, 2006).…”

The Use of Peptide Markers of Carp and Herring Allergens as an Example of Detection of Sequenced and Non-Sequenced Proteins

“…Sarcoplasmic proteins were extracted from both fi sh species by the methods proposed by Bugajska-Schrett er et al (29) and Carrera et al (12). Extraction of sarcoplasmic proteins was carried out using frozen fi sh.…”

“…A peptide should be unique for precursor proteins. Parvalbumins or their fragments are recommended as unique markers of species--specifi c fi sh allergens due to high sequence variability (8,12). A single peptide marker capable of detecting several allergens can also be identifi ed (13).…”

Databases and Associated Bioinformatic Tools in Studies of Food Allergens, Epitopes and Haptens – a Review

“…The database may be suitable for the determination of epitope profi les for potential allergens belonging to families grouping proteins with amino acid sequences containing many common fragments such as caseins [Erhardt et al, 2016], tropomyosins [Marti et al, 2007] or prolamins [Minkiewicz et al, 2015]. For protein families with highly variable sequences such as fi sh parvalbumins [Carrera et al, 2006], the analysis of common epitopes in silico using the BIOPEP database may provide no results.…”