Identification of Colletotrichum species associated with postbloom fruit drop in Brazil through GAPDH sequencing analysis and multiplex PCR

Silva, A. O.; Savi, Daiani Cristina; Gomes, Felipe Bittioli R.; Gos, Francielly Maria Wilke Ramos; Silva, Geraldo José da; Glienke, Chirlei

doi:10.1007/s10658-016-1038-z

Cited by 22 publications

(21 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In recent years, molecular techniques have revolutionized and greatly facilitated the diagnostic of plant pathogens (Kostov et al, 2016;Michalecka et al, 2016;Reich et al, 2016;Silva et al, 2017;Burbank and Ortega, 2018). Among other applications, they have allowed the precise identification of the causal agents of diseases caused by a complex of fungi (Conti et al, 2019), the fulfillment of Koch's postulates with closely related species such as Fusarium spp.…”

Section: Discussionmentioning

confidence: 99%

Discriminant haplotypes of avirulence genes of Phytophthora sojae lead to a molecular assay to predict phenotypes

Dussault‐Benoit

Arsenault‐Labrecque

Sonah

et al. 2020

Molecular Plant Pathology

View full text Add to dashboard Cite

The soybean–Phytophthora sojae interaction operates on a gene‐for‐gene relationship, where the product of a resistance gene (Rps) in the host recognizes that of an avirulence gene (Avr) in the pathogen to generate an incompatible reaction. To exploit this form of resistance, one must match with precision the appropriate Rps gene with the corresponding Avr gene. Currently, this association is evaluated by phenotyping assays that are labour‐intensive and often imprecise. To circumvent this limitation, we sought to develop a molecular assay that would reveal the avirulence allele of the seven main Avr genes (Avr1a, Avr1b, Avr1c, Avr1d, Avr1k, Avr3a, and Avr6) in order to diagnose with precision the pathotypes of P. sojae isolates. For this purpose, we analysed the genomic regions of these Avr genes in 31 recently sequenced isolates with different virulence profiles and identified discriminant mutations between avirulence and virulence alleles. Specific primers were designed to generate amplicons of a distinct size, and polymerase chain reaction conditions were optimized in a final assay of two parallel runs. When tested on the 31 isolates of known virulence, the assay accurately revealed all avirulence alleles. The test was further assessed and compared to a phenotyping assay on 25 isolates of unknown virulence. The two assays matched in 97% (170/175) of the interactions studied. Interestingly, the sole cases of discrepancy were obtained with Avr3a, which suggests a possible imperfect interaction with Rps3a. This molecular assay offers a powerful and reliable tool to exploit and study with greater precision soybean resistance against P. sojae.

show abstract

Section: Discussionmentioning

confidence: 99%

Discriminant haplotypes of avirulence genes of Phytophthora sojae lead to a molecular assay to predict phenotypes

Dussault‐Benoit

Arsenault‐Labrecque

Sonah

et al. 2020

Molecular Plant Pathology

View full text Add to dashboard Cite

show abstract

“…Similarly, C. gloeosporioides was reported as the only Colletotrichum species to cause citrus fruit anthracnose ( Brown 1975 , Timmer et al 2000 ). During the last decade a polyphasic approach was used in several Colletotrichum studies, revealing new species involved with citrus diseases, such as C. abscissum and C. gloeosporioides associated with PFD ( Lima et al 2011 , Crous et al 2015 , Silva et al 2016 ).…”

Section: Discussionmentioning

confidence: 99%

“…A recent extensive investigation in citrus orchards of São Paulo state (Brazil), revealed only C. abscissum and C. gloeosporioides s.str. associated with PFD disease ( Silva et al 2016 ). Key lime anthracnose (KLA), a disease complex relating to leaves, flowers and fruits of Key lime, was initially reported to be caused by C. acutatum ( Brown et al 1996 , Peres et al 2008 , MacKenzie et al 2009 ), but later classified as C. limetticola ( Damm et al 2012a ).…”

Section: Introductionmentioning

confidence: 99%

High species diversity in Colletotrichum associated with citrus diseases in Europe

et al. 2017

View full text Add to dashboard Cite

Species of Colletotrichum are considered important plant pathogens, saprobes, and endophytes on a wide range of plant hosts. Several species are well-known on citrus, either as agents of pre- or post-harvest infections, such as anthracnose, postbloom fruit drop, tear stain and stem-end rot on fruit, or as wither-tip of twigs. In this study we explored the occurrence, diversity and pathogenicity of Colletotrichum spp. associated with Citrus and allied genera in European orchards, nurseries and gardens. Surveys were carried out during 2015 and 2016 in Greece, Italy, Malta, Portugal and Spain. A total of 174 Colletotrichum strains were isolated from symptomatic leaves, fruits, petals and twigs. A multi-locus phylogeny was established based on seven genomic loci (ITS, GAPDH, ACT, CAL, CHS-1, HIS3 and TUB2), and the morphological characters of the isolates determined. Preliminary pathogenicity tests were performed on orange fruits with representative isolates. Colletotrichum strains were identified as members of three major species complexes. Colletotrichum gloeosporioides s.str. and two novel species (C. helleniense and C. hystricis) were identified in the C. gloeosporioides species complex. Colletotrichum karstii, C. novae-zelandiae and two novel species (C. catinaense and C. limonicola) in the C. boninense species complex, and C. acutatum s.str. was also isolated as member of C. acutatum species complex. Colletotrichum gloeosporioides and C. karstii were the predominant species of Colletotrichum isolated. This study represents the first report of C. acutatum on citrus in Europe, and the first detection of C. novae-zelandiae from outside New Zealand. Pathogenicity tests revealed C. gloeosporioides s.str. to be the most virulent species on fruits. The present study improves our understanding of species associated with several disease symptoms on citrus fruits and plants, and provides useful information for effective disease management.

show abstract

“…Diaporthe cf. heveae LGMF1631 also caused excellent inhibition (72%) of the mycelial growth of C. abscissum , the epidemiological agent of Post-bloom Fruit Drop (PFD) (Pinho et al, 2015 ; Silva et al, 2016 ). In favorable climatic conditions, frequent rainy days during the bloom period, PFD can drastically reduce citrus production, reaching approximately 80% (Lima et al, 2011 ).…”

Section: Discussionmentioning

confidence: 99%

Bioprospecting and Structure of Fungal Endophyte Communities Found in the Brazilian Biomes, Pantanal, and Cerrado

et al. 2018

Self Cite

View full text Add to dashboard Cite

Medicinal plants have been recognized as hosts of high diverse endophytic microorganisms, including fungi that produce secondary metabolites with biological activity. Two biomes in Brazil, Pantanal (wetland), and Cerrado (savannah), are known as biodiversity hotspots, and despite their importance as a reservoir for several species, knowledge about the fungal biodiversity in these biomes is very limited. Fungal endophytic communities associated with leaves and petioles of the medicinal plants Vochysia divergens (from Pantanal) and Stryphnodendron adstringens (from Cerrado) were analyzed and studied for their antimicrobial activity against human and plant pathogens. A total of 1,146 isolates of endophytic fungi were obtained from plants collected in January and June of 2016 and grouped into 124 morphotypes. One isolate of each morphotype was identified by sequencing of internal transcribed spacer (ITS) region of the rDNA gene, which revealed the presence of 24 genera, including 3 possible new genera, and 48 taxa. Differences in the endophytic community according to the biomes were observed concerning the analyzed morphotypes. However, when we analyzed the diversity of genera and richness, they were similar for both plants, with Diaporthe, Phyllosticta, and Neofusicoccum as dominant genera. In addition, the community composition of V. divergens differs according to the analyzed plant tissues (petiole and leaf). These data suggested that both, the plant species and plant tissues play a role in the composition of endophytic community. As regards the biotechnological potential, 5 isolates showed activity against the phytopathogens Phyllosticta citricarpa, Colletotrichum abscissum, and Fusarium verticilioides, and 8 isolates showed high activity against clinical pathogens and were selected for the production of crude extract in different culture media. Extract from cultivation of Diaporthe sp. LGMF1548 and LGMF1583 and Neofusicoccum brasiliense LGMF1535 showed activity against methicillin-resistant Staphylococcus aureus, Klebssiella pneumonia, and Candida albicans. In addition, extracts of Diaporthe cf. heveae LGMF1631 inhibited 90% of the mycelial growth of the P. citricarpa and 70% of C. abscissum and may represent an alternative to be used in the biological control of these phytopathogens. Future research will focus on the chemical characterization and structural elucidation of these bioactive compounds.

show abstract

Identification of Colletotrichum species associated with postbloom fruit drop in Brazil through GAPDH sequencing analysis and multiplex PCR

Cited by 22 publications

References 45 publications

Discriminant haplotypes of avirulence genes of Phytophthora sojae lead to a molecular assay to predict phenotypes

Discriminant haplotypes of avirulence genes of Phytophthora sojae lead to a molecular assay to predict phenotypes

High species diversity in Colletotrichum associated with citrus diseases in Europe

Bioprospecting and Structure of Fungal Endophyte Communities Found in the Brazilian Biomes, Pantanal, and Cerrado

Contact Info

Product

Resources

About