Identification of collagen 1α3 in teleost fish species and typical collision induced internal fragmentations

Kleinnijenhuis, Anne J.; Holthoon, Frédérique L. van; Steen, Bastiaan van der

doi:10.1016/j.fochx.2022.100333

Cited by 4 publications

(3 citation statements)

References 31 publications

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“…Besides functional and informational restriction, the maintenance of code robustness [ 33 ] may also play a role during divergence, exemplified by codon usage bias structures. Overall, the current divergence status of bird collagen 1α2 makes it possible to apply generic detection using protein LC-MS. For fish species, however, it was observed that it does not seem feasible to select a comprehensive generic collagen type 1 target, due to the much longer divergence times [ 7 ]. The selection of generic peptides for fish species is still possible, but at a lower level, e.g.…”

Section: Resultsmentioning

confidence: 99%

“…Previously, we developed method modules for the individual detection of quantitative porcine and bovine targets [ 21 ] and for several fish species [ 7 ], amongst others. Generic bird targets should cover as many bird species as possible, but should be different from other animal species.…”

Section: Introductionmentioning

confidence: 99%

“…It is the most abundant protein in vertebrates [1] and consists of triple helices [2]. Usually the helices are heterotrimers of two collagen 1α1 chains and one collagen 1α2 chain [3], although skin type 1 collagens from several bony fish additionally contain a 1α3 chain in the heterotrimer along with 1α1 and 1α2 [4][5][6][7]. Collagen analysis is performed in several fields, e.g.…”

Section: Introductionmentioning

confidence: 99%

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The quest for a generic bird target to detect the presence of bird in food products and considerations for paleoprotein analysis

Kleinnijenhuis¹,

Holthoon²

2022

PLoS ONE

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It can be important for consumers to know whether food products contain animal material and, if so, of which species. Food products with animal material as an ingredient often contain collagen type 1. LC-MS/MS (Liquid Chromatography–tandem Mass Spectrometry) was applied as technique to generically detect bird. Unlike for example fish, that have experienced longer divergence times, it is still possible to find generic LC-MS targets for avian type 1 collagen. After theoretical target selection using 83 collagen 1α2 bird sequences of 33 orders and construction of a common ancestor sequence of birds, experimental evidence was provided by analyzing extracts from 10 extant bird species. Two suitable options have been identified. The combination of VGPIGPAGNR and VGPIGAAGNR (pheasant only) covers all investigated birds and was not found in other species. The peptide EGPVGFpGADGR covers all investigated birds, but also occurs in several species of crocodiles and turtles. The presence of the generic peptide (combination) was confirmed in food products, proving the principle, and can therefore be used to detect the presence of bird. Furthermore, it is shown how the use of constructed ancestor sequences could benefit the field of paleoproteomics, in the interpretation of collagen MS/MS spectra of ancient species. Our theoretical analysis and assessment of reported Brachylophosaurus canadensis collagen 1α2 MS/MS data provided support for several previous peptide sequence assignments, but we also propose that our constructed ancestral bird sequence GPpGESGAVGPAGPIGSR may fit the MS/MS data better than the original assignment GLPGESGAVGPAGPpGSR.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The quest for a generic bird target to detect the presence of bird in food products and considerations for paleoprotein analysis

Kleinnijenhuis¹,

Holthoon²

2022

PLoS ONE

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Characterization of collagen and its hydrolysate from southern bluefin tuna skin and their potencies as DPP-IV inhibitors

Safitri,

Kuziel,

Nagai

et al. 2024

Food Chemistry Advances

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Validation of an analytical method for the quantification of a marker compound and determination of its biological activities in skate skin collagen peptides

Kim¹,

Lee²,

Shin³

et al. 2022

Korean J. Food Preserv.

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In this study, we developed and validated a high-performance liquid chromatography/photodiode array method for the quantification of glycine-proline-hydroxyproline (GPH) as a marker compound in skate skin collagen peptides (SCPs). The accurate molecular mass and amino acid sequence of this marker were determined using a QTOF mass spectrometer. Chromatographic separations were conducted using a 95:5 isocratic mobile phase of 0.1% trifluoroacetic acid (A) and acetonitrile/methanol (1:4 v/v, B) at a flow rate of 0.3 mL/min; the separated compounds were detected at 214 nm using a Jupiter® 4 μm Proteo 90Å column. The method was validated for specificity, linearity, limit of detection (LOD), limit of quantitation (LOQ), accuracy, and precision. It was found to be linear in the range of 1.0-50.0 μg/mL, with a good correlation coefficient (R2=0.9999) and excellent specificity. The LOD and LOQ were 0.07 and 0.22 μg/mL, respectively. A recovery study determined the accuracy of the method, with an average recovery of 103.76%, 100.35% and 103.97% of the marker at 10, 15 and 25 μg/mL, respectively, and a precision study showed a relative standard deviation of less than 2%. Additionally, the DPPH and ABTS radical scavenging activities of the SCPs increased in a concentration-dependent manner, and UV-protective effect was confirmed by human keratinocyte (HaCaT cell) viability. Our study thus provides the foundation for developing functional foods or cosmetics using SCPs.

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Identification of collagen 1α3 in teleost fish species and typical collision induced internal fragmentations

Cited by 4 publications

References 31 publications

The quest for a generic bird target to detect the presence of bird in food products and considerations for paleoprotein analysis

The quest for a generic bird target to detect the presence of bird in food products and considerations for paleoprotein analysis

Characterization of collagen and its hydrolysate from southern bluefin tuna skin and their potencies as DPP-IV inhibitors

Validation of an analytical method for the quantification of a marker compound and determination of its biological activities in skate skin collagen peptides

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