Identification of coiled body-like structures in meristematic cells of Pisum sativum cotyledonary buds

Jennane, A.; Thiry, Marc; Goessens, Guy

doi:10.1007/s004120050360

Cited by 9 publications

(9 citation statements)

References 47 publications

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“…Their composition is also similar. Both nucleolar (NABs) and nucleoplasmic coiled bodies are devoid of DNA (Moreno Díaz de la Espina et al 1982a;Martín et al 1992;Jennane et al 1999). We demonstrate here that both contain RNPs, argyrophilic and highly phosphorylated proteins, splicing factors and fibrillarin, though displaying an intraclass heterogeneity (Alliegro and Alliegro 1998).…”

Section: Ultrastructural Characterisation Of the Two Types Of Coiled mentioning

confidence: 66%

“…(No nucleolus). Bars represent 0.5 µm The structures reported here as coiled bodies in the confocal microscope after immunostaining against components of the splicing and rRNA processing complexes share many features with the previously described plant coiled bodies and NABs (Barlow 1981; Moreno Díaz de la Espina et al 1982a, b;Barlow 1983a, b;Williams et al 1983;Moreno Díaz de la Espina et al 1992;Chamberland and Lafontaine 1993;Jennane et al 1999). Both nucleoplasmic and nucleolar coiled bodies display the same ultrastructural organisation consisting of the typical coiled threads (Monneron and Bernhard 1965;Moreno Díaz de la Espina et al 1982a), which is different from that of G1 prenucleolar bodies containing fibrillarin (Medina et al 1995) and also from the nuclear bodies corresponding to replication factories in plants (Samaniego and Moreno Díaz de la Espina 2000;Samaniego et al 2001).…”

Section: Ultrastructural Characterisation Of the Two Types Of Coiled mentioning

confidence: 70%

“…Nucleoplasmic coiled bodies, which are very rare in dormant cells from primordia, account for the general increase in coiled bodies during activation, although their numbers do not vary significantly between the stabilised and nonstabilised proliferating populations. In contrast, the number of nucleolar coiled bodies in plant cells appears to depend rather on nuclear organisation and/or genetic characteristics than on cell activity (Barlow 1981(Barlow , 1983aLafontaine et al 1991;Lafontaine and Chamberland 1995;Jennane et al 1999). The number of coiled bodies is inversely correlated with their size.…”

Section: Variation Of Coiled Body Numbers During Activation Differs Fmentioning

confidence: 83%

“…Although it was not until the 1980s that coiled bodies were characterised in plants (Moreno Díaz de la Espina et al 1982a, b;Martín et al 1992), in the last decade there has been a great advance in knowledge about plant coiled bodies (Beven et al 1996;Simpson and Filipowicz 1996;Olmedilla et al 1997;Boudonck et al 1998Boudonck et al , 1999Jennane et al 1999). At present we know that plant coiled bodies are highly dynamic structures, with a composition similar to that of animal coiled bodies, whose numbers appear to be cell and developmentally regulated (Boudonck et al 1998) and that move and coalesce "in vivo" (Boudonck et al 1999).…”

Section: Introductionmentioning

confidence: 97%

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Coiled bodies in nuclei from plant cells evolving from dormancy to proliferation

2002

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To characterise the coiled bodies in meristematic nuclei of Saccharum officinarum, immunofluorescence labelling with antibodies against components of the splicing (U2B'' and Sm core protein B) and pre-rRNA processing (fibrillarin) complexes was used in cells from the dormant root primordia and from roots at different times after activation to the steady state of proliferation. The number, size and distribution of coiled bodies varied in the meristematic tissue depending on cell activity. While G0 cells in the dry primordia and proliferating cells showed a similar number of coiled bodies attached to their nucleoli, the number of nucleoplasmic coiled bodies greatly increased after the primordia were stimulated to proliferate. Their number remained steady from the time the meristematic population reached the steady state of proliferation, as estimated by flow cytometry. Fractionation studies demonstrated that coiled bodies are a part of the underlying nuclear matrix. Comparison of immunocytochemical and cytochemical data from confocal and electron microscopical studies demonstrated that the nucleolar and nucleoplasmic coiled bodies detected by confocal microscopy shared many features, suggesting that they form a family of closely related structures.

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Section: Ultrastructural Characterisation Of the Two Types Of Coiled mentioning

confidence: 66%

Section: Ultrastructural Characterisation Of the Two Types Of Coiled mentioning

confidence: 70%

Section: Variation Of Coiled Body Numbers During Activation Differs Fmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 97%

See 2 more Smart Citations

Coiled bodies in nuclei from plant cells evolving from dormancy to proliferation

2002

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“…Such structures have been reported in other vertebrate groups as well as in insects, plants and yeast (Jennane et al, 1999;Varheggen et al, 2000;Bogolyubov and Parfenov, 2008). Cajal bodies are enriched in snRNP and snoRNP and play a role in their maturation (Cioce and Lamond, 2005;Morris, 2008).…”

Section: Discussionmentioning

confidence: 89%

Nucleolar structure across evolution: The transition between bi- and tricompartmentalized nucleoli lies within the class Reptilia

Lamaye

Galliot

Alibardi

et al. 2011

Journal of Structural Biology

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Two types of nucleolus can be distinguished among eukaryotic cells: a tri-compartmentalized nucleolus in amniotes and a bi-compartmentalized nucleolus in all the others. However, though the nucleolus' ultrastructure is well characterized in mammals and birds, it has been so far much less studied in reptiles. In this work, we examined the ultrastructural organization of the nucleolus in various tissues from different reptilian species (three turtles, three lizards, two crocodiles, and three snakes). Using cytochemical and immunocytological methods, we showed that in reptiles both types of nucleolus are present: a bi-compartmentalized nucleolus in turtles and a tri-compartmentalized nucleolus in the other species examined in this study. Furthermore, in a given species, the same type of nucleolus is present in all the tissues, however, the importance and the repartition of those nucleolar components could vary from one tissue to another. We also reveal that, contrary to the mammalian nucleolus, the reptilian fibrillar centers contain small clumps of condensed chromatin and that their surrounding dense fibrillar component is thicker. Finally, we also report that Cajal bodies are detected in reptiles. Altogether, we believe that these results have profound evolutionarily implications since they indicate that the point of transition between bipartite and tripartite nucleoli lies at the emergence of the amniotes within the class Reptilia.

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Nuclear bodies and compartmentalization of pre-mRNA splicing factors in higher plants

et al. 2004

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We studied the fine structural organization of nuclear bodies in the root meristem during germination of maize and Arabidopsis thaliana using electron microscopy (EM). Cajal bodies (CBs) were observed in quiescent embryos and germinating cells in both species. The number and distribution of CBs were investigated. To characterize the nuclear splicing domains, immunofluorescence labelling with antibodies against splicing factors (U2B 00 and m3G-snRNAs) and in situ hybridisation (with U1/U6 antisense probes) were performed combined with confocal microscopy. Antibodies specific to the Arabidopsis SR splicing factor atRSp31 were produced. AtRSp31 was detected in quiescent nuclei and in germinating cells. This study revealed an unexpected speckled nuclear organization of atRSp31 in root epidermal cells where micro-clusters of interchromatin granules were also observed by EM. Therefore, we examined the distribution of green fluorescent protein (GFP)-tagged atRSp31 in living cells after Agrobacterium -mediated transient expression. When expressed transiently, atRSp31-GFP exhibited a speckled distribution in leaf cells. Treatments with a-amanitin, okadaic acid, staurosporine or heat shock induced the speckles to reorganize. Furthermore, we generated stable Arabidopsis transgenics expressing atRSp31-GFP. The distribution of the fusion protein was identical to that of endogenous atRSp31. Three-dimensional time-lapse confocal microscopy showed that speckles were highly dynamic domains over time.

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Identification of coiled body-like structures in meristematic cells of Pisum sativum cotyledonary buds

Cited by 9 publications

References 47 publications

Coiled bodies in nuclei from plant cells evolving from dormancy to proliferation

Coiled bodies in nuclei from plant cells evolving from dormancy to proliferation

Nucleolar structure across evolution: The transition between bi- and tricompartmentalized nucleoli lies within the class Reptilia

Nuclear bodies and compartmentalization of pre-mRNA splicing factors in higher plants

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