Identification of Circulating miR-22-3p and miR-93-5p as Stable Endogenous Control in Tuberculosis Study

Korma, Workneh; Mihret, Adane; Tarekegn, Azeb; Chang, Yunhee; Hwang, Dasom; Tessema, Tesfaye Sisay; Lee, Hyeyoung

doi:10.3390/diagnostics10110868

Cited by 11 publications

(11 citation statements)

References 39 publications

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“…We guess the reason for this difference is that the situation of HCC patients and the composition of the control group in our study are different from their projects. Korma et al 55 found that miR-22-3p and miR-93-5p are stable reference genes for plasma microRNA analysis in tuberculosis study which is consistent with our results (Figure 4B). Therefore, we predict that miR-22 may become a stable expression reference gene in liver disease research, which needs more data to verify.…”

Section: Discussionsupporting

confidence: 92%

Circulating microRNAs (miR‐16, miR‐22, miR‐122) expression and early diagnosis of hepatocellular carcinoma

Fang

Yan

Wang

et al. 2022

Clinical Laboratory Analysis

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Purpose: Circulating microRNA (miRNA) has been reported to have diagnostic value in multiple tumors. To identify serum miRNAs for early diagnosis of hepatocellular carcinoma (HCC), we analyzed the differential miRNA expression between HCC patients and controls.Methods: Real-time reverse transcription polymerase chain reaction (RT-PCR) was carried out to detect serum miR-16, miR-22, and miR-122 expression in 100 HCC patients and 100 controls (including hepatitis B, liver cirrhosis, liver metastases, hepatic hemangioma, health group, and each of them had 20 subjects). The miRNA expression results were combined with alpha-fetoprotein (AFP) to evaluate the diagnostic efficacy in HCC through receiver operating characteristic (ROC) curve. And the target genes were predicted through bioinformatics methods.Results: Compared with controls, the expression of miR-16 and miR-122 significantly increased in early-stage HCC patients, while no significant changes were detected in miR-22. The ROC curve analysis demonstrated that miR-16 and miR-122 had a high diagnostic efficacy (AUC 0.798 and 0.759), and it was improved when combined with AFP (AUC 0.862). When compared with each of the five groups in the controls, the results showed that miR-16 of HCC was significantly higher than liver cirrhosis (AUC 0.936), liver metastases, and health; miR-122 was significantly higher than liver metastases, hepatitis B, and health. Moreover, 175 and 101 potential target genes were regulated by miR-16 and miR-122, respectively. And most of the target genes were enriched in the PI3K, MAPK, FoxO signaling pathways, and pathways in cancer. Conclusion:Our findings illustrate that both circulating miR-16 and miR-122 can provide value for early diagnosis of HCC and they are potential biomarkers for the earlystage HCC.

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Section: Discussionsupporting

confidence: 92%

Circulating microRNAs (miR‐16, miR‐22, miR‐122) expression and early diagnosis of hepatocellular carcinoma

Fang

Yan

Wang

et al. 2022

Clinical Laboratory Analysis

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“…To calculate the miRNA relative fold changes, the ΔΔCt method was used with miR-93 as an endogenous control. This stable internal reference has recently been recommended for human cancer cell lines and plasma samples [ 34 , 35 ].…”

Section: Methodsmentioning

confidence: 99%

Different bisphenols induce non-monotonous changes in miRNA expression and LINE-1 methylation in two cell lines

Oldenburg

Fürhacker

Hartmann

et al. 2021

Environmental Epigenetics

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4,4ʹ-Isopropylidenediphenol (bisphenol A, BPA), a chemical substance that is widely used mainly as a monomer in the production of polycarbonates, in epoxy resins, and in thermal papers, is suspected to cause epigenetic modifications with potentially toxic consequences. Due to its negative health effects, BPA is banned in several products and is replaced by other bisphenols such as bisphenol S and bisphenol F. The present study examined the effects of BPA, bisphenol S, bisphenol F, p,pʹ-oxybisphenol, and the BPA metabolite BPA β-d-glucuronide on the expression of a set of microRNAs (miRNAs) as well as long interspersed nuclear element-1 methylation in human lung fibroblast and Caco-2 cells. The results demonstrated a significant modulation of the expression of different miRNAs in both cell lines including miR-24, miR-155, miR-21, and miR-146a, known for their regulatory functions of cell cycle, metabolism, and inflammation. At concentrations between 0.001 and 10 µg/ml, especially the data of miR-155 and miR-24 displayed non-monotonous and often significant dose–response curves that were U- or bell-shaped for different substances. Additionally, BPA β-d-glucuronide also exerted significant changes in the miRNA expression. miRNA prediction analysis indicated effects on multiple molecular pathways with relevance for toxicity. Besides, long interspersed nuclear element-1 methylation, a marker for the global DNA methylation status, was significantly modulated by two concentrations of BPA and p,pʹ-oxybisphenol. This pilot study suggests that various bisphenols, including BPA β-d-glucuronide, affect epigenetic mechanisms, especially miRNAs. These results should stimulate extended toxicological studies of multiple bisphenols and a potential use of miRNAs as markers.

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“…In the present study, a total of 11 reference genes ( Figure 7 ) including nine miRNAs, one snRNA, and one piRNA were found in six stains ( Table 2 ). Among them, RF00026 (U6) [ 15 , 50 ], let-7a [ 59 ], let-7i [ 9 ], let-7g [ 9 ], miR-22-3p [ 57 ], miR-26a-5p [ 58 ], and miR-451a [ 9 ] have been reported, and let-7f-5p, let-7b-5p, miR-181a-5p, piR-020829 were newly identified. However, as illustrated in Figure 7 , although there were relatively similar expression profiles between samples of the same fluid or tissue type, samples of different body fluids or tissues did not exhibit consistent expression levels.…”

Section: Resultsmentioning

confidence: 99%

A Comprehensive Characterization of Small RNA Profiles by Massively Parallel Sequencing in Six Forensic Body Fluids/Tissue

Liu

Wang

et al. 2022

Genes

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Body fluids/tissue identification (BFID) is an essential procedure in forensic practice, and RNA profiling has become one of the most important methods. Small non-coding RNAs, being expressed in high copy numbers and resistant to degradation, have great potential in BFID but have not been comprehensively characterized in common forensic stains. In this study, the miRNA, piRNA, snoRNA, and snRNA were sequenced in 30 forensic relevant samples (menstrual blood, saliva, semen, skin, venous blood, and vaginal secretion) using the BGI platform. Based on small RNA profiles, relative specific markers (RSM) and absolute specific markers (ASM) were defined, which can be used to identify a specific body fluid/tissue out of two or six, respectively. A total of 5204 small RNAs were discovered including 1394 miRNAs (including 236 novel miRNA), 3157 piRNAs, 636 snoRNAs, and 17 snRNAs. RSMs for 15 pairwise body fluid/tissue groups were discovered by differential RNA analysis. In addition, 90 ASMs that were specifically expressed in a certain type of body fluid/tissue were screened, among them, snoRNAs were reported first in forensic genetics. In brief, our study deepened the understanding of small RNA profiles in forensic stains and offered potential BFID markers that can be applied in different forensic scenarios.

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Identification of Circulating miR-22-3p and miR-93-5p as Stable Endogenous Control in Tuberculosis Study

Cited by 11 publications

References 39 publications

Circulating microRNAs (miR‐16, miR‐22, miR‐122) expression and early diagnosis of hepatocellular carcinoma

Circulating microRNAs (miR‐16, miR‐22, miR‐122) expression and early diagnosis of hepatocellular carcinoma

Different bisphenols induce non-monotonous changes in miRNA expression and LINE-1 methylation in two cell lines

A Comprehensive Characterization of Small RNA Profiles by Massively Parallel Sequencing in Six Forensic Body Fluids/Tissue

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