Identification of Circulating Melanoma Cells in Uveal Melanoma Patients by Dual-Marker Immunoenrichment

Tura, Aysegül; Lüke, Julia; Merz, Hartmut; Reinsberg, Mihaela; Lüke, Matthias; Jager, Martine J.; Grisanti, Salvatore

doi:10.1167/iovs.14-14512

Cited by 35 publications

(53 citation statements)

References 20 publications

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“…Different technical approaches have been used to detect or isolate UM CTCs, including immunomagnetic sorting [332], enrichment by size of tumor cells [333], or RT-PCR detection of tyrosinase and MelanA/MART1 mRNA [334]. Variable results have been reported on the prognostic value of CTC detection in UM, and comparisons among studies are difficult due to the different methodologies applied.…”

Section: Biomarkersmentioning

confidence: 99%

“…Also, the combination of two monoclonal antibodies against a melanoma-associated glycoprotein was used for CTC immunomagnetic sorting from the blood of 31 UM patients without metastatic disease. The presence or number of CTCs did not show a significant association with clinical prognostic factors [332]. One recent study combined the isolation of CTCs with the same technology with immuno-FISH detection of monosomy of chromosome 3 in 44 primary UM patients.…”

Section: Biomarkersmentioning

confidence: 99%

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The biology of uveal melanoma

Amaro

Gangemi

Piaggio

et al. 2017

Cancer Metastasis Rev

186

193

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Uveal melanoma (UM), a rare cancer of the eye, is distinct from cutaneous melanoma by its etiology, the mutation frequency and profile, and its clinical behavior including resistance to targeted therapy and immune checkpoint blockers. Primary disease is efficiently controlled by surgery or radiation therapy, but about half of UMs develop distant metastasis mostly to the liver. Survival of patients with metastasis is below 1 year and has not improved in decades. Recent years have brought a deep understanding of UM biology characterized by initiating mutations in the G proteins GNAQ and GNA11. Cytogenetic alterations, in particular monosomy of chromosome 3 and amplification of the long arm of chromosome 8, and mutation of the BRCA1-associated protein 1, BAP1, a tumor suppressor gene, or the splicing factor SF3B1 determine UM metastasis. Cytogenetic and molecular profiling allow for a very precise prognostication that is still not matched by efficacious adjuvant therapies. G protein signaling has been shown to activate the YAP/TAZ pathway independent of HIPPO, and conventional signaling via the mitogen-activated kinase pathway probably also contributes to UM development and progression. Several lines of evidence indicate that inflammation and macrophages play a pro-tumor role in UM and in its hepatic metastases. UM cells benefit from the immune privilege in the eye and may adopt several mechanisms involved in this privilege for tumor escape that act even after leaving the niche. Here, we review the current knowledge of the biology of UM and discuss recent approaches to UM treatment.

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Section: Biomarkersmentioning

confidence: 99%

Section: Biomarkersmentioning

confidence: 99%

The biology of uveal melanoma

Amaro

Gangemi

Piaggio

et al. 2017

Cancer Metastasis Rev

186

193

View full text Add to dashboard Cite

show abstract

“…MCSP-Expression, keine CD45-Expression) gut von Leukozyten (hohe CD45-Expression) abgrenzen. Daneben bieten sich aber auch die Form und Größe des Nukleus als weitere Unterscheidungsmerkmale an [30] mithilfe eines Antikörpers, der gegen das CD146 gerichtet ist. Für die Abgrenzung gegen Leukozyten und Endothelzellen erfolgten weitere Immunfluoreszenzfärbungen [44].…”

Section: Nachweismethoden Zirkulierender Melanomzellen (Zmz) Bei Chorunclassified

Potenziale und Limitationen der Detektion von zirkulierenden Melanomzellen

2018

Self Cite

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ZusammenfassungDie Bestimmung und Charakterisierung zirkulierender Melanomzellen ist ein nicht invasives und komplikationsarmes Verfahren mit dem Potenzial, einen choroidalen Nävus von einem choroidalen Melanom abzugrenzen und frühzeitig einer Therapie zuführen zu können. Zukünftig bietet es die Chance, das therapeutische Ansprechen zu überwachen und bei der Therapieauswahl zu helfen. Es besteht die Hoffnung, weitere Erkenntnisse hinsichtlich des Metastasierungsprozesses zu gewinnen und damit Ansatzpunkte für die Entwicklung zielgerichteter Therapien. In diesem Artikel werden die verschiedenen Techniken der Bestimmung zirkulierender Tumorzellen vorgestellt und ihr Potenzial bzw. Limitationen diskutiert.

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“…Der Vorteil dieser Methode ist die Möglichkeit weiterer Untersuchun-gen wie z. B. die geno-und phänotypische Charakterisierung [29][30][31]. Mit unserer Methodik werden ZMZs mittels an Immunobeads gekoppelten Antikörpern, die gegen verschiedene melanomspezifische Antigene gerichtet sind, aus dem Blut isoliert.…”

Section: Nachweismethoden Zirkulierender Melanomzellen (Zmz) Bei Chorunclassified

Potenziale und Limitationen der Detektion von zirkulierenden Melanomzellen

Lüke

Grisanti

Tura

2017

Klin Monatsbl Augenheilkd

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The detection and characterisation of circulating melanoma cells (CMC) is a non-invasive procedure with a low complication rate and can potentially distinguish choroidal nevi from uveal melanomas and help to initiate early adequate therapy. It has the perspective to select and monitor treatment. This technique may lead to new insights into the metastasis process itself and help to detect specific targets for future therapies. This article introduces the different techniques for CMC detection; their potentials and limitations are discussed.

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Identification of Circulating Melanoma Cells in Uveal Melanoma Patients by Dual-Marker Immunoenrichment

Cited by 35 publications

References 20 publications

The biology of uveal melanoma

The biology of uveal melanoma

Potenziale und Limitationen der Detektion von zirkulierenden Melanomzellen

Potenziale und Limitationen der Detektion von zirkulierenden Melanomzellen

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