Identification of circulating extracellular vesicle long RNAs as diagnostic biomarkers for patients with severe acute pancreatitis

Abstract

Dear Editor,Severe acute pancreatitis (SAP) may cause multiple organ dysfunction and results in the systemic inflammatory response syndrome. 1 The contrast-enhanced computed tomography (CECT) is the main diagnostic method in clinical practice, but has several disadvantages such as time delay, missed diagnosis and high false-positive rate, and renal burden caused by contrast agents. 2 Since SAP has a high mortality, the novel detection methods and noninvasive biomarkers are urgently needed for timely, rapid, and precision SAP diagnosis.Small extracellular vesicles (sEVs), transport of biological molecules (RNAs, proteins, and metabolites) from disease-related organs or tissues to target cells for cell-tocell communication has been demonstrated as a potential source for biomarker discovery. 3 Here, we have performed F I G U R E 1 Transcriptomic analysis of circulating extracellular vesicles (EVs) for severe acute pancreatitis (SAP) diagnosis. (A) EVs are secreted by pancreatic tissue entering the blood circulation. (B) EV isolation using EXODUS. (C) EV transcriptomic analysis using RNA sequencing. (D) Identification of the key regulators from MAP to SAP by integrative analysis of EV transcriptomics and metabolomics. Qingfu Zhu and Rui Yang contributed equally to this work.