Identification of circular RNAs and corresponding regulatory networks reveals potential roles in the brains of honey bee workers exposed to dinotefuran

Huang, Minjie; Dong, Jie; Guo, Haiyang; Xiao, Minghui; Wang, Deqian

doi:10.1016/j.pestbp.2021.104994

Cited by 6 publications

(6 citation statements)

References 56 publications

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“…The potential targeting relationships between the DEcircRNAs and DEmiRNAs, as well as those between the DEmiRNAs and DEmRNAs, were predicted using a combination of the TargetFind software (Kiełbasa et al 2010 ) and mirTarBase software (Huang et al 2022a , b ). On the basis of the predicted targeting relationships, DEcircRNA-DEmiRNA-DEmRNA regulatory networks were constructed and then visualized using the Cytoscape v.3.2.1 software (Smoot et al 2011 ) with default parameters.…”

Section: Methodsmentioning

confidence: 99%

“…Antioxidant enzymes like superoxide dismutase (SOD), catalase (CAT), and glutathione S -transferase (GST) were used as weapons by insects to combat pathogens or parasites (Ding et al 2001 ; Zhang et al 2023b ). Based on the Nr annotations, similarly, the potential targeting relationships between antioxidant enzyme–associated mRNAs and DEmiRNAs, as well as between DEmiRNAs and DEcircRNAs, were predicted with Targetfinder (Kiełbasa et al 2010 ) and mirTarBase software (Huang et al 2022a , 2022 ). Further, DEcircRNA-DEmiRNA-mRNA regulatory networks were constructed and then visualized by Cytoscape v.3.2.1 software (Smoot et al 2011 ).…”

Section: Methodsmentioning

confidence: 99%

“…Compared with Drosophila and B. mori , understanding of circRNA in honey bees is fairly limited. Huang et al ( 2022a , 2022 ) identified 33, 144, and 211 DEcircRNAs by resolving the differential expression profiles of Apis mellifera ligustica workers at 1, 5, and 10 days after dinotefuran exposure. Further DEcircRNA-miRNA-mRNA regulatory network analysis showed that circ_0008898 and circ_0001829 were potentially involved in the host immune response as “molecular sponges” of miRNAs.…”

Section: Introductionmentioning

confidence: 99%

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Dynamics and regulatory role of circRNAs in Asian honey bee larvae following fungal infection

Guo,

Zhang,

Zang

et al. 2024

Appl Microbiol Biotechnol

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Non-coding RNA (ncRNA) plays a vital part in the regulation of immune responses, growth, and development in plants and animals. Here, the identification, characteristic analysis, and molecular verification of circRNAs in Apis cerana cerana worker larval guts were conducted, followed by in-depth investigation of the expression pattern of larval circRNAs during Ascosphaera apis infection and exploration of the potential regulatory part of differentially expressed circRNAs (DEcircRNAs) in host immune responses. A total of 3178 circRNAs in the larval guts of A. c. cerana were identified, with a length distribution ranging from 15 to 96,007 nt. Additionally, 155, 95, and 86 DEcircRNAs were identified in the in the 4-, 5-, and 6-day-old larval guts following A. apis infection. These DEcircRNAs were predicted to target 29, 25, and 18 parental genes relevant to 12, 20, and 17 GO terms as well as 144, 114, and 61 KEGG pathways, including 5 cellular and 4 humoral immune pathways. Complex competing endogenous RNA (ceRNA) regulatory networks were detected as being formed among DEcircRNAs, DEmiRNAs, and DEmRNAs. The target DEmRNAs were engaged in 36, 47, and 47 GO terms as well as 331, 332, and 331 pathways, including 6 cellular and 6 humoral immune pathways. Further, 19 DEcircRNAs, 5 DEmiRNAs, and 3 mRNAs were included in the sub-networks relative to 3 antioxidant enzymes. Finally, back-splicing sites within 15 circRNAs and the difference in the 15 DEcircRNAs’ expression between uninoculated and A. apis–inoculated larval guts were confirmed based on molecular methods. These findings not only enrich our understanding of bee host–fungal pathogen interactions but also lay a foundation for illuminating the mechanism underlying the DEcircRNA-mediated immune defense of A. c. cerana larvae against A. apis invasion. Key points • The expression pattern of circRNAs was altered in the A. cerana worker larval guts following A. apis infection. • Back-splicing sites within 15 A. cerana circRNAs were verified using molecular approaches. DEcircRNAs potentially modulated immune responses and antioxidant enzymes in A. apis–challenged host guts.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Dynamics and regulatory role of circRNAs in Asian honey bee larvae following fungal infection

Guo,

Zhang,

Zang

et al. 2024

Appl Microbiol Biotechnol

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“…Like all other neonicotinoids, the most controversial issue with DNF is that bees are adversely affected by the toxic effects of DNF. For this reason, many studies have been conducted on these effects in recent years [44][45][46][47].…”

Section: Dnfmentioning

confidence: 99%

Determination of neonicotinoid pesticides nitenpyram and dinotefuran by electroanalytical methods

Kul

2023

Explor Foods Foodomics

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Nitenpyram (NIT) and dinotefuran (DNF) are neonicotinoid pesticides commonly used in the production and storage of agricultural products, as well as in forests and gardens, for the purpose of protection from insect pests. Although they are safer for mammals, their toxic effects on pollinators, such as bees, and their long-term accumulation in water and soil, are important problems. Therefore, it is crucial to ensure that their usage remains within certain specific limits and that their residues are quickly, precisely, and reliably monitored in various samples. In this review, electrochemical methods, which are voltammetry, amperometry, and potentiometry, for the determination of NIT and DNF in pure solutions, agricultural, and environmental samples by using various modified electrodes were reviewed. The results obtained from studies published since 2011 were compared, and the effectiveness of the selected methods was demonstrated. It was observed that the electrochemical methods, particularly voltammetry, used in the studies conducted for NIT and DNF yielded selective and sensitive results at detection limits at nmol L–1 levels. These methods also exhibited high precision and accuracy without being affected by the matrix of the studied samples, such as soil, water, or agricultural products.

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“…Compared with Drosophila and B. mori, understanding of circRNA in honey bees is fairly limited. Huang et al [19] identified 33, 144, and 211 DEcircRNAs by resolving the differential expression profiles of Apis mellifera ligustica workers at 1, 5 and 10 days after dinotefuran exposure. Further DEcircRNA-miRNA-mRNA regulatory network analysis showed that circ_0008898 and circ_0001829 were potentially involved in the host immune response as "molecular sponges" of miRNAs.…”

Section: Introductionmentioning

confidence: 99%

CircRNAs and their regulatory networks associated with the antioxidant enzymes and immune responses of Asian honey bee larvae to fungal infection

Guo

Zhang

Gao

et al. 2023

Preprint

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Non–coding RNA (ncRNA) plays an important role in the regulation of immune responses, growth, and development in plants and animals. Here, the identification, characteristic investigation, and molecular verification of circRNAs in Apis cerana cerana larval guts were conducted, and the expression pattern of larval circRNAs during Ascosphaera apis infection was analyzed. This was followed by exploration of the potential regulatory part of differentially expressed circRNAs (DEcircRNAs) in host immune responses. A total of 3178 circRNAs in the larval guts of A. c. cerana were identified, with a length distribution ranging from 15 nt to 96007 nt. Additionally, 45, 33, and 48 up-regulated circRNAs, as well as 110, 62, and 38 down-regulated circRNAs were identified in the A. apis–inoculated 4–, 5–, and 6–day–old larval guts in comparison with the corresponding uninoculated larval guts. These DEcircRNAs were predicted to target 29, 25, and 18 parental genes, which were relative to 12, 20, and 17 GO terms as well as 144, 114, and 61 KEGG pathways, including five cellular and four humoral immune pathways containing melanization, phagosomes, lysosomes, endocytosis, apoptosis, MAPK, Ras, and Jak–STAT signaling pathways. Furthermore, complex competing endogenous RNA (ceRNA) regulatory networks were detected as being formed among DEcircRNAs, DEmiRNAs, and DEmRNAs. The target DEmRNAs were engaged in 36, 47, and 47 GO terms as well as 331, 332, and 331 pathways, including six cellular and six humoral immune-related pathways. In total, nineteen DEcircRNAs, five DEmiRNAs, and three mRNAs were included in the sub-networks relative to three antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST). Finally, back–splicing sites within 15 circRNAs and the difference in the 15 DEcircRNAs expression between uninoculated and A.apis–inoculated larval guts were confirmed utilizing molecular methods. These findings not only enrich our understanding of bee host–fungal pathogen interactions, but also lay a foundation for illuminating the mechanism underlying the DEcircRNA–mediated immune defense of A. c. cerana larvae against A. apis invasion.

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Identification of circular RNAs and corresponding regulatory networks reveals potential roles in the brains of honey bee workers exposed to dinotefuran

Cited by 6 publications

References 56 publications

Dynamics and regulatory role of circRNAs in Asian honey bee larvae following fungal infection

Dynamics and regulatory role of circRNAs in Asian honey bee larvae following fungal infection

Determination of neonicotinoid pesticides nitenpyram and dinotefuran by electroanalytical methods

CircRNAs and their regulatory networks associated with the antioxidant enzymes and immune responses of Asian honey bee larvae to fungal infection

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