Identification of circadian rhythms in <i>Nannochloropsis</i> species using bioluminescence reporter lines

Poliner, Eric; Cummings, Cameron; Newton, Linsey; Farré, Eva M.

doi:10.1111/tpj.14314

Cited by 21 publications

(28 citation statements)

References 120 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cas9-mediated target gene disruption was conducted using an episomal CRISPR system 51 . The sgRNAs were designed to meet the following criteria: (1) uniqueness of target sequence and PAM (final 12 bp + NGG) in genome, (2) location within the first 300 bp of the gene, and (3) target sequence present in both genome and mRNA sequence (to avoid targeting introns).…”

Section: Methodsmentioning

confidence: 99%

Role of an ancient light-harvesting protein of PSI in light absorption and photoprotection

Gan

Iwai

et al. 2021

Nat Commun

View full text Add to dashboard Cite

Diverse algae of the red lineage possess chlorophyll a-binding proteins termed LHCR, comprising the PSI light-harvesting system, which represent an ancient antenna form that evolved in red algae and was acquired through secondary endosymbiosis. However, the function and regulation of LHCR complexes remain obscure. Here we describe isolation of a Nannochloropsis oceanica LHCR mutant, named hlr1, which exhibits a greater tolerance to high-light (HL) stress compared to the wild type. We show that increased tolerance to HL of the mutant can be attributed to alterations in PSI, making it less prone to ROS production, thereby limiting oxidative damage and favoring growth in HL. HLR1 deficiency attenuates PSI light-harvesting capacity and growth of the mutant under light-limiting conditions. We conclude that HLR1, a member of a conserved and broadly distributed clade of LHCR proteins, plays a pivotal role in a dynamic balancing act between photoprotection and efficient light harvesting for photosynthesis.

show abstract

Section: Methodsmentioning

confidence: 99%

Role of an ancient light-harvesting protein of PSI in light absorption and photoprotection

Gan

Iwai

et al. 2021

Nat Commun

View full text Add to dashboard Cite

show abstract

“…Robust diel rhythms in growth, gene expression, pigment synthesis, phototactic movements, and bioluminescence have been observed in a variety of phytoplanktonic organisms, including diatoms (7–10). Diatoms represent the most species-rich group of algae in the ocean and populate a wide range of aquatic environments (11).…”

mentioning

confidence: 99%

bHLH-PAS protein RITMO1 regulates diel biological rhythms in the marine diatom Phaeodactylum tricornutum

Annunziata

Ritter

Fortunato

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Periodic light–dark cycles govern the timing of basic biological processes in organisms inhabiting land as well as the sea, where life evolved. Although prominent marine phytoplanktonic organisms such as diatoms show robust diel rhythms, the mechanisms regulating these processes are still obscure. By characterizing aPhaeodactylum tricornutumbHLH-PAS nuclear protein, hereby named RITMO1, we shed light on the regulation of the daily life of diatoms. Alteration of RITMO1 expression levels and timing by ectopic overexpression results in lines with deregulated diurnal gene expression profiles compared with the wild-type cells. Reduced gene expression oscillations are also observed in these lines in continuous darkness, showing that the regulation of rhythmicity by RITMO1 is not directly dependent on light inputs. We also describe strong diurnal rhythms of cellular fluorescence in wild-type cells, which persist in continuous light conditions, indicating the existence of an endogenous circadian clock in diatoms. The altered rhythmicity observed in RITMO1 overexpression lines in continuous light supports the involvement of this protein in circadian rhythm regulation. Phylogenetic analysis reveals a wide distribution of RITMO1-like proteins in the genomes of diatoms as well as in other marine algae, which may indicate a common function in these phototrophs. This study adds elements to our understanding of diatom biology and offers perspectives to elucidate timekeeping mechanisms in marine organisms belonging to a major, but under-investigated, branch of the tree of life.

show abstract

“…This system efficiently generates targeted mutations and allows the loss of episomal DNA after removing the selection pressure, resulting in marker-free non-transgenic engineered lines. In the study reported by Poliner et al, 2019 [16], the nitrate reductase gene (NR) has been disrupted and subsequently, the CRISPR episome has been successfully removed to generate non-transgenic marker-free nitrate reductase knock-out lines (NR-KO).…”

Section: Episomal Crispr Systemmentioning

confidence: 99%

“…Despite the simplicity of microalgae cells, few of the CRISPR-Cas-mediated transformation and genetic editing have been successful ( [14][15][16]). Through all the attempts and trials to edit target genes in microalgae, many pitfalls have been encountered harming and slowing down the research in this field.…”

Section: Introductionmentioning

confidence: 99%

Genome Editing by CRISPR-Cas: A Game Change in the Genetic Manipulation of Chlamydomonas

Ghribi

Nouemssi

Meddeb-Mouelhi

et al. 2020

Life

View full text Add to dashboard Cite

Microalgae are promising photosynthetic unicellular eukaryotes among the most abundant on the planet and are considered as alternative sustainable resources for various industrial applications. Chlamydomonas is an emerging model for microalgae to be manipulated by multiple biotechnological tools in order to produce high-value bioproducts such as biofuels, bioactive peptides, pigments, nutraceuticals, and medicines. Specifically, Chlamydomonas reinhardtii has become a subject of different genetic-editing techniques adapted to modulate the production of microalgal metabolites. The main nuclear genome-editing tools available today include zinc finger nucleases (ZFNs), transcriptional activator-like effector nucleases (TALENs), and more recently discovered the clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated protein (Cas) nuclease system. The latter, shown to have an interesting editing capacity, has become an essential tool for genome editing. In this review, we highlight the available literature on the methods and the applications of CRISPR-Cas for C. reinhardtii genetic engineering, including recent transformation methods, most used bioinformatic tools, best strategies for the expression of Cas protein and sgRNA, the CRISPR-Cas mediated gene knock-in/knock-out strategies, and finally the literature related to CRISPR expression and modification approaches.

show abstract

Identification of circadian rhythms in Nannochloropsis species using bioluminescence reporter lines

Cited by 21 publications

References 120 publications

Role of an ancient light-harvesting protein of PSI in light absorption and photoprotection

Role of an ancient light-harvesting protein of PSI in light absorption and photoprotection

bHLH-PAS protein RITMO1 regulates diel biological rhythms in the marine diatom Phaeodactylum tricornutum

Genome Editing by CRISPR-Cas: A Game Change in the Genetic Manipulation of Chlamydomonas

Contact Info

Product

Resources

About