Identification of ciliary epithelial‐specific genes using subtractive libraries and cDNA arrays in the avian eye

Kubota, Ryo; McGuire, Christopher Roger; Dierks, Blair; Reh, Thomas A.

doi:10.1002/dvdy.20000

Cited by 31 publications

(33 citation statements)

References 39 publications

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“…A combination of subtractive hybridization and cDNA microarrays were used to identify candidate genes and ISH was employed subsequently to validate the specificity of expression of these candidate markers. The majority of the genes identified as specific markers of the ciliary epithelium by Kubota et al (2004) did not overlap with those from our study. Only three genes (Zic2, Tgfb2, and Collagen IX) were commonly isolated by the two approaches.…”

Section: Comparison Of Ciliary Body Marker Studiescontrasting

confidence: 56%

“…These approaches have included ISH screens, subtractive hybridization strategies, and microarrays using mouse, human, and chick anterior segment structures (Thut et al, 2001;Escribano and CocaPrados, 2002;Kubota et al, 2004;Diehn et al, 2005). In the current study, a combination of single-cell transcriptional profiling and ISH was used to identify markers of the ciliary body in the developing mouse and chick retina.…”

Section: Comparison Of Ciliary Body Marker Studiesmentioning

confidence: 99%

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Identification of genes expressed preferentially in the developing peripheral margin of the optic cup

Trimarchi

Cho

Cepko

2009

Developmental Dynamics

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Specification of the peripheral optic cup by Wnt signaling is critical for formation of the ciliary body/iris. Identification of marker genes for this region during development provides a starting point for functional analyses. During transcriptional profiling of single cells from the developing eye, two cells were identified that expressed genes not found in most other single cell profiles. In situ hybridizations demonstrated that many of these genes were expressed in the peripheral optic cup in both early mouse and chicken development, and in the ciliary body/iris at subsequent developmental stages. These analyses indicate that the two cells probably originated from the developing ciliary body/iris. Changes in expression of these genes were assayed in embryonic chicken retinas when canonical Wnt signaling was ectopically activated by CA-␤-catenin. Twelve ciliary body/iris genes were identified as upregulated following induction, suggesting they are excellent candidates for downstream effectors of Wnt signaling in the optic cup. Developmental

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Section: Comparison Of Ciliary Body Marker Studiescontrasting

confidence: 56%

Section: Comparison Of Ciliary Body Marker Studiesmentioning

confidence: 99%

Identification of genes expressed preferentially in the developing peripheral margin of the optic cup

Trimarchi

Cho

Cepko

2009

Developmental Dynamics

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“…[27][28][29][30][31] Collagen II is essential for the gel character of the vitreous. 31,32 The dominant origin of the vitreous proteins is the ciliary body, 30,31,[33][34][35][36] with little contribution of the Figure 4 The retinal neuroepithelium does not contribute in the de novo assembly of inner limiting membrane (ILM). Transplantation of chick retinal tissue in organ-cultured quail host eyes shows a tight integration of the graft into the host after 24 h of culture (a).…”

Section: Vitreous and The Origin Of Vb Proteins In The Eyementioning

confidence: 99%

“…Transferrin, a protein typical for serum, is present in large quantities in VB. It is synthesized by the ciliary body, 34,39 and its concentration in VB remains steady from embryogenesis to old age. 22 A similar time course was detected for fibronectin.…”

Section: Vitreous and The Origin Of Vb Proteins In The Eyementioning

confidence: 99%

Origin and turnover of ECM proteins from the inner limiting membrane and vitreous body

Halfter

Dong

et al. 2008

Eye

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The inner limiting membrane (ILM) and the vitreous body (VB) are two major extracellular matrix (ECM) structures that are essential for early eye development. The ILM is considered to be the basement membrane of the retinal neuroepithelium, yet in situ hybridization and chick/quail transplant experiments in organcultured eyes showed that all components critical for ILM assembly, such as laminin or collagen IV, are not synthesized by the retina. Rather, ILM proteins, with the exception of agrin, originate from the lens or (and) ciliary body and are shed into the vitreous. The VB serves as a reservoir providing high concentrations of ILM proteins for the instant assembly of new ILM during rapid embryonic eye growth. The function of the retina in ILM assembly is to provide the cellular receptor proteins for the binding of the ILM proteins from the vitreous. The VB is a gelatinous ECM structure that fills the vitreous cavity of the eye. Its major structural proteins, collagen II and fibrillin, originate primarily from the ciliary body. Reverse transcription-PCR and western blotting show that the rate of synthesis of structural, monomeric ILM and VB proteins, such as laminin, collagen IV and II is very high during embryogenesis and very low in the adult. The downregulation of ILM and VB protein synthesis occurs during early postnatal life, and both ILM and VB are from then on maintained throughout life with minimum turnover. Our data explain why ILM and VB do not regenerate after vitrectomy and ILM peeling.

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“…Quantitative RT-PCR was performed as previously described (Kubota et al, 2004) using an Opticon monitor from MJ Research and SYBR Green PCR Master Mix (Applied Biosystems). RNA samples were taken from three different individuals at the ages indicated.…”

Section: Quantitative Rt Pcrmentioning

confidence: 99%

Epidermal growth factor receptor expression regulates proliferation in the postnatal rat retina

Liu

Gümüşçü

et al. 2006

Glia

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109

121

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Epidermal growth factor (EGF) is known to promote proliferation of both retinal progenitors and Muller glia in vitro, but several questions remain concerning an in vivo role for this factor. In this study, we investigated whether the EGF receptor (EGFR) is necessary for the maintenance of normal levels of progenitor and Muller glial proliferation in vivo. Here, we show that (1) mice with homozygous deletion of the Egfr gene have reduced proliferation in late stages of retinal histogenesis, (2) EGF is mitogenic for M€ uller glia in vivo during the first two postnatal weeks in the rodent retina, (3) the effectiveness of EGF as a M€ uller glial mitogen declines in parallel with the decline in EGFR expression as the retina matures, and (4) following damage to the retina from continuous light exposure, EGFR expression is upregulated in M€ uller glia to levels close to those in the neonatal retina, resulting in a renewed mitotic response to EGF. Together with previous results from other studies, these data indicate that the downregulation of a growth factor receptor is one mechanism by which glial cells maintain mitotic quiescence in the mature nervous system. V V C 2006 Wiley-Liss, Inc.

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Identification of ciliary epithelial‐specific genes using subtractive libraries and cDNA arrays in the avian eye

Cited by 31 publications

References 39 publications

Identification of genes expressed preferentially in the developing peripheral margin of the optic cup

Identification of genes expressed preferentially in the developing peripheral margin of the optic cup

Origin and turnover of ECM proteins from the inner limiting membrane and vitreous body

Epidermal growth factor receptor expression regulates proliferation in the postnatal rat retina

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