Identification of cholinergic chemosensory cells in mouse tracheal and laryngeal glandular ducts

Krasteva‐Christ, Gabriela; Soultanova, Aichurek; Schütz, Burkhard; Papadakis, Tamara; Weiß, Christian; Deckmann, Klaus; Chubanov, Vladimir; Gudermann, Thomas; Voigt, Anja; Meyerhof, Wolfgang; Boehm, Ulrich; Weihe, Eberhard; Kummer, Wolfgang

doi:10.1016/j.intimp.2015.05.028

Cited by 16 publications

(9 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While evaluating the expression of GFP by peripheral cholinergic neurons was the primary goal of this study, we also observed prominent staining for GFP in scattered epithelial cells of the airways and in the small intestine of the same preparations that were used to evaluate the neuronal localization of GFP and VAChT. This observation confirms previous findings from other studies that used the same ChAT BAC -eGFP transgenic mice to examine cholinergic epithelial cells (Kummer and Krasteva-Christ, 2014; Krasteva-Christ et al, 2015; Schütz et al, 2015). Analysis of double-labeled tissues by confocal microscopy revealed that some GFP+ epithelial cells in the airways exhibited low to moderate intensity of staining for VAChT, but epithelial cells in the small intestine lacked VAChT.…”

Section: Discussionsupporting

confidence: 88%

Variable expression of GFP in different populations of peripheral cholinergic neurons of ChATBAC-eGFP transgenic mice

Brown¹,

Bond²,

Hoover³

2018

Autonomic Neuroscience

View full text Add to dashboard Cite

Immunohistochemistry is used widely to identify cholinergic neurons, but this approach has some limitations. To address these problems, investigators developed transgenic mice that express enhanced green fluorescent protein (GFP) directed by the promoter for choline acetyltransferase (ChAT), the acetylcholine synthetic enzyme. Although, it was reported that these mice express GFP in all cholinergic neurons and non-neuronal cholinergic cells, we could not detect GFP in cardiac cholinergic nerves in preliminary experiments. Our goals for this study were to confirm our initial observation and perform a qualitative screen of other representative autonomic structures for the presences of GFP in cholinergic innervation of effector tissues. We evaluated GFP fluorescence of intact, unfixed tissues and the cellular localization of GFP and vesicular acetylcholine transporter (VAChT), a specific cholinergic marker, in tissue sections and intestinal whole mounts. Our experiments identified two major tissues where cholinergic neurons and/or nerve fibers lacked GFP: 1) most cholinergic neurons of the intrinsic cardiac ganglia and all cholinergic nerve fibers in the heart and 2) most cholinergic nerve fibers innervating airway smooth muscle. Most cholinergic neurons in airway ganglia stained for GFP. Cholinergic systems in the bladder and intestines were fully delineated by GFP staining. GFP labeling of input to ganglia with long preganglionic projections (vagal) was sparse or weak, while that to ganglia with short preganglionic projections (spinal) was strong. Total absence of GFP might be due to splicing out of the GFP gene. Lack of GFP in nerve projections from GFP-positive cell bodies might reflect a transport deficiency.

show abstract

Section: Discussionsupporting

confidence: 88%

Variable expression of GFP in different populations of peripheral cholinergic neurons of ChATBAC-eGFP transgenic mice

Brown¹,

Bond²,

Hoover³

2018

Autonomic Neuroscience

View full text Add to dashboard Cite

show abstract

“…1C). A hallmark of SCCs in many other tissues is the use of acetylcholine as a downstream effector 17,22,27,28 . ChAT-GFP mice, expressing GFP from the promoter of the acetylcholine-synthesizing enzyme choline acetyltransferase (ChAT), showed a population of GFP+ cells in the marginal epithelium that did not overlap with the α-gustducin+ populations in the junctional epithelium, indicating that gSCCs are not cholinergic (Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

“…In the gut, tuft or brush cells (a type of SCC) 25,26 detect and evoke innate immune responses against helminthic parasites 18 . SCCs serve as innate immune sentinels in a variety of anatomical locations, including but not limited to gastric mucosa and biliary tract 27 , tracheal and laryngeal glandular ducts 28 , and urinary tract 17 .…”

Section: Introductionmentioning

confidence: 99%

Gingival solitary chemosensory cells are immune sentinels for periodontitis

et al. 2019

View full text Add to dashboard Cite

Solitary chemosensory cells (SCCs) are epithelial sentinels that utilize bitter Tas2r receptors and coupled taste transduction elements to detect pathogenic bacterial metabolites, triggering host defenses to control the infection. Here we report that SCCs are present in mouse gingival junctional epithelium, where they express several Tas2rs and the taste signaling components α-gustducin (Gnat3), TrpM5, and Plcβ2. Gnat3−/− mice have altered commensal oral microbiota and accelerated naturally occurring alveolar bone loss. In ligature-induced periodontitis, knockout of taste signaling molecules or genetic absence of gingival SCCs (gSCCs) increases the bacterial load, reduces bacterial diversity, and renders the microbiota more pathogenic, leading to greater alveolar bone loss. Topical treatment with bitter denatonium to activate gSCCs upregulates the expression of antimicrobial peptides and ameliorates ligature-induced periodontitis in wild-type but not in Gnat3−/− mice. We conclude that gSCCs may provide a promising target for treating periodontitis by harnessing innate immunity to regulate the oral microbiome.

show abstract

“…C. Denatonium increased PTS significantly (black line). 24,36 Additionally, we detected high levels of Gng13 (Guanine nucleotide-binding protein subunit gamma-13) and Pou2f3 (a transcription factor), which is regulating the generation of different chemosensory cells including tuft cells in the gut and BC in the airways. Denatonium did not influence PTS in the presence of the cholinergic receptor inhibitors mecamylamine (MEC) and atropine (grey line).…”

Section: F I G U R Ementioning

confidence: 98%

Tracheal brush cells release acetylcholine in response to bitter tastants for paracrine and autocrine signaling

et al. 2019

View full text Add to dashboard Cite

This is an open access article under the terms of the Creat ive Commo ns Attri butio n-NonCo mmercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. AbstractFor protection from inhaled pathogens many strategies have evolved in the airways such as mucociliary clearance and cough. We have previously shown that protective respiratory reflexes to locally released bacterial bitter "taste" substances are most probably initiated by tracheal brush cells (BC). Our single-cell RNA-seq analysis of murine BC revealed high expression levels of cholinergic and bitter taste signaling transcripts (Tas2r108, Gnat3, Trpm5). We directly demonstrate the secretion of acetylcholine (ACh) from BC upon stimulation with the Tas2R agonist denatonium. Inhibition of the taste transduction cascade abolished the increase in [Ca 2+ ] i in | 317 HOLLENHORST ET aL.

show abstract

Identification of cholinergic chemosensory cells in mouse tracheal and laryngeal glandular ducts

Cited by 16 publications

References 57 publications

Variable expression of GFP in different populations of peripheral cholinergic neurons of ChATBAC-eGFP transgenic mice

Variable expression of GFP in different populations of peripheral cholinergic neurons of ChATBAC-eGFP transgenic mice

Gingival solitary chemosensory cells are immune sentinels for periodontitis

Tracheal brush cells release acetylcholine in response to bitter tastants for paracrine and autocrine signaling

Contact Info

Product

Resources

About