Identification of chlamydial species in crocodiles and chickens by PCR-HRM curve analysis

Robertson, Timothy James; Bibby, Summa; O’Rourke, Denise; Belfiore, T.; Agnew-Crumpton, Rebecca; Noormohammadi, Amir H.

doi:10.1016/j.vetmic.2010.04.007

Cited by 39 publications

(38 citation statements)

References 16 publications

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“…In the past five years, researchers described the occurrence of C. psittaci and atypical Chlamydia in chickens (8,9,12,24,25,26,27). Thus, Chlamydia infections seem to be (re)-emerging in Australian, Chinese, and European broilers and to a lesser extent in layers, although poultry farmers mostly do not seem to be aware of it.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, Chlamydia infections seem to be (re)-emerging in Australian, Chinese, and European broilers and to a lesser extent in layers, although poultry farmers mostly do not seem to be aware of it. Some of these papers document clinical disease in chickens (8,24,25,26,27), while others report no clinical disease in chickens (9,12) but zoonotic transfer and pneumonia in humans (9). Thus, less is known on the pathogenicity of C. psittaci strains in chickens.…”

Section: Discussionmentioning

confidence: 99%

“…Surprisingly, all chicken flocks appeared healthy. Recent data suggest that this new chlamydial agent could putatively be widespread in Australian, French, Greek, Croatian, Slovenian, and Chinese chicken flocks (12,28).…”

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confidence: 99%

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Pathogenicity of Low and Highly VirulentChlamydia psittaciIsolates for Specific-Pathogen-Free Chickens

Yin¹,

Lagae²,

Kalmar³

et al. 2013

Avian Diseases

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Pathogenicity of Low and Highly VirulentChlamydia psittaciIsolates for Specific-Pathogen-Free Chickens

Yin¹,

Lagae²,

Kalmar³

et al. 2013

Avian Diseases

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“…The following strains were used: C. psittaci, genotype B, strain 10/423 (isolated from a broiler with pneumonia from a Belgian farm), C. psittaci, genotype B, strain 10/525 (originated from another Belgian broiler farm, also associated with pneumonia), and C. psittaci, genotype D, strain 10/298 (originated from a broiler with pneumonia from a French farm) (24). Molecular characteristics was performed by a genotype-specific real-time PCR, allowing the identification of the outer membrane protein A (ompA) genotypes A to F and E/B (8), and by Multi Locus Sequence Typing (MLST) using seven housekeeping genes (enoA, fumC, gatA, gidA, hemN, hlfX, oppA) (12). The strains were grown in Buffalo Green Monkey (BGM) cells, as previously described (19).…”

Section: Methodsmentioning

confidence: 99%

Chlamydia Psittaci Strains from Broiler Chickens Induce Histopathological Lesions and Mortality in SPF Chickens

Yin

Kalmar

Chiers

et al. 2015

Bulletin of the Veterinary Institute in Pulawy

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A detailed study on histopathological lesions induced by two C. psittaci outer membrane protein A (ompA) genotype B strains (10/423 and 10/525) and one genotype D strain (10/298) in experimentally infected (aerosol) specific pathogen free (SPF) chickens was performed. The strains were derived from Belgian and French commercially raised broilers with pneumonia. Both genotype B and D strains induced conjunctivitis, rhinitis, sinusitis, tracheitis, bronchitis, pneumonitis, airsacculitis, splenitis, hepatitis, nephritis, and enteritis in sequentially (days 2 to 34 post infection) euthanized chickens. Inflammation of the ovaries was only observed in genotype D infected chickens. Overall, the genotype D strain caused more severe gross and histopathological lesions and mortality (54.5%) early upon infection. The genotype D strain seemed to replicate faster as severity of the lesions increased more quickly. C. psittaci is a primary pathogen in chickens, and efficient monitoring and control of this emerging zoonotic pathogen is urgently needed.

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“…Ever since applying NAATs, C. psittaci has been detected more often in chickens. Virulent C. psittaci strains were detected by NAATs, and isolated from diseased chickens raised in Australia, Belgium, China, France and Germany (Gaede et al, 2008;Zhang et al, 2008;Laroucau et al, 2009;Robertson et al, 2010;Zhou et al, 2010;Yin et al, 2013a). Recently, C. psittaci was detected in a Belgian chicken slaughterhouse and in a Belgian chicken hatchery.…”

Section: Introductionmentioning

confidence: 99%

Longitudinal monitoring for respiratory pathogens in broiler chickens reveals co-infection of Chlamydia psittaci and Ornithobacterium rhinotracheale

Boeck

Kalmar

Dumont

et al. 2015

Journal of Medical Microbiology

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Chlamydia psittaci is prevalent in broiler chicken production. However, the role of C. psittaci in the respiratory disease complex needs to be clarified. Our aim was to identify the time point when a C. psittaci infection appeared on a broiler farm and to examine the presence of other respiratory pathogens at that time. We focused on the 'major' respiratory pathogens occurring in Belgian broilers, namely infectious bronchitis virus (IBV), avian metapneumovirus (aMPV), Ornithobacterium rhinotracheale, Mycoplasma gallisepticum and Mycoplasma synoviae, and examined their co-occurrence with C. psittaci on three commercial broiler farms. For all farms, 1-day-old broilers showed high maternal antibody titres against C. psittaci in the presence of viable C. psittaci. Maternal antibodies seemed to protect against respiratory signs. Maternal antibodies declined and clinical outbreaks could be identified serologically even before maternal antibodies completely disappeared. Mixed infections with genotypes B/C and B/C/D were observed. Broilers with C. psittaci antibody increases showed conjunctivitis, signs of upper respiratory disease and dyspnoea. C. psittaci always preceded an O. rhinotracheale infection. Infections with aMPV, IBV or Mycoplasma spp. were not observed. Evidence was provided that C. psittaci could occur at an early age in broilers without a predisposing respiratory infection. Both C. psittaci and O. rhinotracheale should be considered when developing prevention strategies for respiratory disease in broilers

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Identification of chlamydial species in crocodiles and chickens by PCR-HRM curve analysis

Cited by 39 publications

References 16 publications

Pathogenicity of Low and Highly VirulentChlamydia psittaciIsolates for Specific-Pathogen-Free Chickens

Pathogenicity of Low and Highly VirulentChlamydia psittaciIsolates for Specific-Pathogen-Free Chickens

Chlamydia Psittaci Strains from Broiler Chickens Induce Histopathological Lesions and Mortality in SPF Chickens

Longitudinal monitoring for respiratory pathogens in broiler chickens reveals co-infection of Chlamydia psittaci and Ornithobacterium rhinotracheale

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