Identification of chemotaxis operon cheYZA and cheA gene expression under stressful conditions in Piscirickettsia salmonis

Albornoz, Romina; Valenzuela, Karla; Pontigo, Juan Pablo; Sánchez, Patricio; Ruiz, Pamela; Avendaño‐Herrera, Rubén; Romero, Alex; Oliver, Cristian; Yáñez, Alejandro J.

doi:10.1016/j.micpath.2017.04.030

Cited by 21 publications

(26 citation statements)

References 43 publications

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“…In the present study, we used comparative genomics to analyze chromosomal sequence of four isolates to determine the common genetic content and organization, unique virulence attributes, and evolutionary relationship with other strains. Whole-genome sequence analysis of S. algae detected the presence of chemotaxis gene cluster cheYZA operon that is conserved in the chemotactic bacteria [35]. Chemotaxis is a directed motility in response to concentration gradients of signals.…”

Section: Discussionmentioning

confidence: 99%

Comparative Genomics Reveals Pathogenicity-Related Loci in Shewanella algae

Wang

Huang

et al. 2020

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Shewanella algae is an emerging marine zoonotic pathogen and accounts for considerable mortality and morbidity in compromised hosts. However, there is scarce literature related to the understanding of the genetic background of virulence determinants in S. algae. In this study, we aim to determine the occurrence of common virulence genes in S. algae using whole-genome sequence and comparative genomic analysis. Comparative genomics reveals putative-virulence genes related to bile resistance, chemotaxis, hemolysis, and motility. We detected the existence of hlyA, hlyD, and hlyIII involved in hemolysis. We also found chemotaxis gene cluster cheYZA operon and cheW gene. The results provide insights into the genetic basis underlying pathogenicity in S. algae.

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Section: Discussionmentioning

confidence: 99%

Comparative Genomics Reveals Pathogenicity-Related Loci in Shewanella algae

Wang

Huang

et al. 2020

Canadian Journal of Infectious Diseases and Medical Microbiolog

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“…Then, plates were incubated at 37 °C to fix the adhered biofilm, followed by incubation for 10 min with crystal violet (200 µL at 3 g/mL). The CV solution was then discarded, and the wells were washed with PBS 1× for 5 min by repeated pipetting to remove dye excess and dried upside-down at room temperature for 30 min [ 35 ]. Finally, images of the CV stained biofilm obtained for each culture condition were registered using an inverted microscope (100×) coupled to an Idu Labcam Microscopy Adapter.…”

Section: Methodsmentioning

confidence: 99%

Biofilm Produced In Vitro by Piscirickettsia salmonis Generates Differential Cytotoxicity Levels and Expression Patterns of Immune Genes in the Atlantic Salmon Cell Line SHK-1

et al. 2020

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Piscirickettsia salmonis is the causative agent of Piscirickettsiosis, an infectious disease with a high economic impact on the Chilean salmonid aquaculture industry. This bacterium produces biofilm as a potential resistance and persistence strategy against stressful environmental stimuli. However, the in vitro culture conditions that modulate biofilm formation as well as the effect of sessile bacteria on virulence and immune gene expression in host cells have not been described for P. salmonis. Therefore, this study aimed to analyze the biofilm formation by P. salmonis isolates under several NaCl and iron concentrations and to evaluate the virulence of planktonic and sessile bacteria, together with the immune gene expression induced by these bacterial conditions in an Atlantic salmon macrophage cell line. Our results showed that NaCl and Fe significantly increased biofilm production in the LF-89 type strain and EM-90-like isolates. Additionally, the planktonic EM-90 isolate and sessile LF-89 generated the highest virulence levels, associated with differential expression of il-1β, il-8, nf-κb, and iκb-α genes in SHK-1 cells. These results suggest that there is no single virulence pattern or gene expression profile induced by the planktonic or sessile condition of P. salmonis, which are dependent on each strain and bacterial condition used.

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“…), and a relationship between biofilm formation and genes related to chemotaxis has been suggested (Albornoz et al . ). More specifically, P. salmonis expresses the cheA chemotaxis gene when cultivated in media known to induce biofilm production.…”

Section: Piscirickettsia Salmonismentioning

confidence: 97%

“…Gene expressions related to flagella structures have been reported in P. salmonis (Rojas et al 2013;Carril et al 2017), and a relationship between biofilm formation and genes related to chemotaxis has been suggested (Albornoz et al 2017). More specifically, P. salmonis expresses the cheA chemotaxis gene when cultivated in media known to induce biofilm production.…”

Section: P Salmonis: Virulence and Pathogenicitymentioning

confidence: 99%

Addressing viral and bacterial threats to salmon farming in Chile: historical contexts and perspectives for management and control

Figueroa

Cárcamo

Yáñez

et al. 2019

Reviews in Aquaculture

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The rapid growth of the salmon farming industry in Chile has led to the appearance of various viral, bacterial, parasitic, and fungal pathogens affecting farmed fish. The Chilean salmon industry has suffered several health crises associated with high fish mortalities, such as caused by the infectious salmon anaemia virus (2007) and harmful algal blooms (2016). In addition to these events, marine farms are continuously affected by outbreaks of harmful pathogens, including the bacteria Piscirickettsia salmonis and, most recently, a reappearance of Renibacterium salmoninarum, and the infectious pancreatic necrosis virus. These outbreaks have led to stricter regulations, but the salmon farming industry continues to struggle despite reforms. In addition to the aforementioned pathogens, which are a continuous threat, other apparently under control pathogens have also reappeared in recent years. In this review, we analyse the current state of knowledge on four of the main pathogens affecting salmon farming in Chile. The infectious pancreatic necrosis virus is relevant as it affects freshwater‐stage fish, with survivors then acting as carriers. The infectious salmon anaemia virus currently appears to be under control; however, P. salmonis and R. salmoninarum continue to be the cause for high mortalities in the Chilean aquaculture industry.

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Identification of chemotaxis operon cheYZA and cheA gene expression under stressful conditions in Piscirickettsia salmonis

Cited by 21 publications

References 43 publications

Comparative Genomics Reveals Pathogenicity-Related Loci in Shewanella algae

Comparative Genomics Reveals Pathogenicity-Related Loci in Shewanella algae

Biofilm Produced In Vitro by Piscirickettsia salmonis Generates Differential Cytotoxicity Levels and Expression Patterns of Immune Genes in the Atlantic Salmon Cell Line SHK-1

Addressing viral and bacterial threats to salmon farming in Chile: historical contexts and perspectives for management and control

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