Identification of chemosensory gene families in<i>Rhyzopertha dominica</i>(Coleoptera: Bostrichidae)

Diakite, M.M.; Wang, Juan; Ali, Saqib; Wang, Man‐Qun

doi:10.4039/tce.2015.13

Cited by 9 publications

(22 citation statements)

References 37 publications

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“…Transcriptome analysis of D. ponderosae found a total of 31 candidate OBPs, but one third of them were not detected in the antennal cDNA library (Andersson et al, 2013). And in I. typographus, M. alternatus, D. helophoroides, and R. dominica, 15, 29, 23, and 16 transcripts encoding putative OBPs were annotated (Andersson et al, 2013;Wang et al, 2014;Diakite et al, 2015). Therefore, the number of LdecOBPs identified in this study is consistent with previous reports.…”

Section: Discussionsupporting

confidence: 90%

“…In this study, we annotated olfactory genes in a Coleopteran pest, L. decemlineata, through antennal transcriptome sequence. Compared with six previously reported beetle antennal transcriptomes (Mitchell et al, 2012;Andersson et al, 2013;Wang et al, 2014;Diakite et al, 2015) sequenced by 454 or Illumina platform, the depth of sequencing of this L. decemlineata antennal transcriptome was greater. The length of the assembled transcripts varied obviously in these seven beetles and the N50 of our transcripts is longer than those in M. caryae (Mitchell et al, 2012), I. typographus (Andersson et al, 2013), M. alternatus (Wang et al, 2014) and D. helophoroides (Wang et al, 2014), but shorter than the transcripts in D. ponderosae (Andersson et al, 2013).…”

Section: Discussionmentioning

confidence: 70%

“…About 40% of all described insect species are beetles (about 400,000 species). In this, the largest insect order, olfactory genes have been identified from a few species: one from the genome of T. castaneum (Richards et al, 2008;Kim et al, 2010), and recently from the antennal transcriptomes of Megacyllene caryae (Mitchell et al, 2012), Ips typographus (Andersson et al, 2013), Dendroctonus ponderosae (Andersson et al, 2013), Monochamus alternatus (Wang et al, 2014), Dastarcus helophoroides (Wang et al, 2014), and Rhyzopertha dominica (Diakite et al, 2015). Thus, a greater effort must be made to investigate other beetle species in order to better understand the molecular biology of Coleopteran and insect olfaction.…”

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confidence: 99%

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Identification of candidate olfactory genes in Leptinotarsa decemlineata by antennal transcriptome analysis

et al. 2015

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The sense of smell is critical for the survival of insects, by as insects detect odor signals in the environment and make appropriate behavioral responses such as host preference, mate choice, and oviposition site selection. The antenna is the main olfactory organ in insects. Multiple antennal proteins have been suggested to be involved in olfactory signal transduction pathway such as odorant receptors (ORs), ionotropic receptors (IRs), odorant binding proteins (OBPs), chemosensory proteins (CSPs) and sensory neuron membrane proteins (SNMPs). In this study, we identified several olfactory gene subfamilies in the economically important Coleopteran agricultural pest, Leptinotarsa decemlineata, by assembling the adult male and female antennal transcriptomes. In the male and female antennal transcriptome, we identified a total of 37 OR genes, 10 IR genes, 26 OBP genes, 15 CSP genes, and 3 SNMP genes. Further, expression of all candidate ORs was validated in male or female antenna by semi-quantitative reverse transcription PCR. Most of the candidate OR genes have similar expression levels in male and female. A few OR genes have been detected to have male-specific (LdecOR6) or male-biased (LdecOR5, LdecOR12, LdecOR26, and LdecOR32) expression. Additionally, two OR genes (LdecOR3 and LdecOR29) were observed to be expressed higher in female. Our findings make it possible for future research of the olfactory system of L. decemlineata at the molecular level.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 70%

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confidence: 99%

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Identification of candidate olfactory genes in Leptinotarsa decemlineata by antennal transcriptome analysis

et al. 2015

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“…The above unigenes may play a significant part in antennal chemosensory processes, such as transporter activity and odorant binding. The 42 AgerORs identified in A. germari are similar to the 43 OR genes in A. corpulenta and I. typographus (Andersson et al, 2013), fewer than the 57 ORs reported in M. caryae (Mitchell et al, 2012) or the 73 transcripts encoding ORs in the antennal transcriptomes of P. striolata (Wu et al, 2016) and the 53 ORs in A. chinensis but more than the nine ORs identified in M. alternatus (Wang et al, 2014), six ORs in Rhyzopertha dominica (Diakite et al, 2016), or 37 ORs in A. glabripennis (Hu et al, 2016) belonging to seven known coleopteran specific subgroups. There were seven coleopteran specific subgroups reported in previous studies (Engsontia et al, 2008; FIGURE 4 | Molecular phylogeny comparing AgerORs with odorant receptors (ORs) from 10 insect species.…”

Section: Discussionmentioning

confidence: 68%

Identification and Expression Profile of Olfactory Receptor Genes Based on Apriona germari (Hope) Antennal Transcriptome

Qian

Mang

et al. 2020

Front. Physiol.

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Insects' olfactory receptor plays a central role in detecting chemosensory information from the environment. Odorant receptors (ORs) and ionotropic receptors (IRs) are two types of olfactory receptors, and they are essential for the recognition of ligands at peripheral neurons. Apriona germari (Hope) (Coleoptera: Cerambycidae) is one of the most serious insect pests that cause damage to economic trees and landscaping trees, resulting in massive environmental damages and economic losses. Olfactorybased management strategy has been suggested as a promising strategy to control this wood-boring beetle. However, the olfactory perception mechanism in A. germari is now almost unknown. In the present study, RNA sequencing analysis was used to determine the transcriptomes of adult A. germari antennae. Among 36,834 unigenes derived from the antennal assembly, we identified 42 AgerORs and three AgerIRs. Based on the tissue expression pattern analysis, 27 AgerORs displayed a female-biased expression. Notably, AgerOR3, 5, 13, 33, and 40 showed a significant female-biased expression and were clustered with the pheromone receptors of Megacyllene caryae in the phylogenetic tree, suggesting that these AgerORs could be potential pheromone receptors for sensing male-produced sex pheromones in A. germari. The AgerIRs expression profile demonstrated that AgerIR2 had high expression levels in male labial palps, suggesting that this receptor may function to detect female-deposited trail-sex pheromone blend of A. germari. In addition, the phylogenetic tree showed that the Orco gene of five cerambycidae species was highly conservative. These results provide a foundation for further studies on the molecular mechanisms of olfactory chemoreception in A. germari apart from suggesting novel targets for the control of this pest in the future.

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“…Kairomones, or food odors, can attract a wider range of species and both sexes, but need to compete against other food odors in the environment [ 62 ]. Sequenced genomes and transcriptomes from stored product insects are providing data on sensory genes that will lead to the development of better lures [ 66 , 67 ].…”

Section: Application In Pest Managementmentioning

confidence: 99%

Gene Disruption Technologies Have the Potential to Transform Stored Product Insect Pest Control

Perkin

Adrianos

Oppert

2016

Insects

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Stored product insects feed on grains and processed commodities manufactured from grain post-harvest, reducing the nutritional value and contaminating food. Currently, the main defense against stored product insect pests is the pesticide fumigant phosphine. Phosphine is highly toxic to all animals, but is the most effective and economical control method, and thus is used extensively worldwide. However, many insect populations have become resistant to phosphine, in some cases to very high levels. New, environmentally benign and more effective control strategies are needed for stored product pests. RNA interference (RNAi) may overcome pesticide resistance by targeting the expression of genes that contribute to resistance in insects. Most data on RNAi in stored product insects is from the coleopteran genetic model, Tribolium castaneum, since it has a strong RNAi response via injection of double stranded RNA (dsRNA) in any life stage. Additionally, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology has been suggested as a potential resource for new pest control strategies. In this review we discuss background information on both gene disruption technologies and summarize the advances made in terms of molecular pest management in stored product insects, mainly T. castaneum, as well as complications and future needs.

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Identification of chemosensory gene families inRhyzopertha dominica(Coleoptera: Bostrichidae)

Cited by 9 publications

References 37 publications

Identification of candidate olfactory genes in Leptinotarsa decemlineata by antennal transcriptome analysis

Identification of candidate olfactory genes in Leptinotarsa decemlineata by antennal transcriptome analysis

Identification and Expression Profile of Olfactory Receptor Genes Based on Apriona germari (Hope) Antennal Transcriptome

Gene Disruption Technologies Have the Potential to Transform Stored Product Insect Pest Control

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