Identification of Charcot-Leyden Crystals by Electron Microscopy

Carson, Henry J.; Buschmann, Robert J.; Weisz-Carrington, Paul; Choi, Yeun Sook

doi:10.3109/01913129209057825

Cited by 19 publications

(6 citation statements)

References 23 publications

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“…Several types of spontaneously forming eosinophilic crystals have been described in the mammalian literature. Charcot-Leyden crystals (CLCs) are commonly described in human conditions of eosinophilia, such as allergy, parasitic infection, eosinophilic granuloma and various neoplasms (Charcot & Robin 1853, Dvorak et al 1990, Carson et al 1992. CLCs are composed of the enzyme lysophospholipase (lysolecithin acylhydrolase, EC 3.1.1.5) (Weller et al 1982), which spontaneously forms regular geometric structures, usually hexagonal bipyramids, when released from damaged or degranulating eosinophils (El-Hashimi 1971).…”

Section: Discussionmentioning

confidence: 99%

Ultrastructure of Mycobacterium marinum granuloma in striped bass Morone saxatilis

Gauthier

Vogelbein²,

Ottinger³

2004

Dis. Aquat. Org.

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An emerging epizootic of mycobacteriosis currently threatens striped bass Morone saxatilis populations in Chesapeake Bay, USA. Several species of mycobacteria, including Mycobacterium marinum, species resembling M. avium, M. gordonae, M. peregrinum, M. scrofulaceum and M. terrae, and the new species M. shottsii have been isolated from diseased and healthy bass. In this study, we describe the ultrastructure of developing M. marinum granulomas in experimentally infected bass over a period of 45 wk. The primary host response to injected mycobacteria was formation of large macrophage aggregations containing phagocytosed bacilli. M. marinum were always contained within phagosomes. Close association of lysosomes with mycobacterial phagosomes, as well as the presence of electron-opaque material within phagosomes, suggested phagolysosomal fusion. Development of granulomas involved epithelioid transformation of macrophages, followed by appearance of central necrosis. Desmosomes were present between mature epithelioid cells. The necrotic core region of M. marinum granulomas was separated from overlying epithelioid cells by several layers of flattened, electron-opaque spindle-shaped cells. These cells appeared to be formed by compression of epithelioid cells and, aside from a flattened nucleus, did not possess recognizable organelles. Following the development of well-defined, paucibacillary granulomas, secondary disease was observed. Recrudescence was marked by bacterial replication followed by disruption of granuloma architecture, including loss of epithelioid and spindle cell layers. In advanced recrudescent lesions, normal tissue was replaced by macrophages, fibroblasts, and other inflammatory leukocytes. Large numbers of mycobacteria were observed, both intracellular and suspended in cellular debris. KEY WORDS: Mycobacteriosis · Striped bass · Morone saxatilis · Mycobacterium marinum · Granuloma · Ultrastructure Resale or republication not permitted without written consent of the publisherDis Aquat Org 62: [121][122][123][124][125][126][127][128][129][130][131][132] 2004 body granulomas are composed of a solid mass of epithelioid cells and giant cells, with the eliciting agent often visible in the center. The other major granuloma type, the immune granuloma, is formed in response to insoluble particles that stimulate a cell-mediated immune response, such as mycobacteria. Cytokines produced by T cells, such as IL-12 and interferon-γ, play an important role in formation and maintenance of immune granulomas (Cooper et al. 1993, Ehlers et al. 2000. A prominent feature of immune granulomas produced by mycobacteria such as Mycobacterium tuberculosis is caseous necrosis in the lesion core, which produces a highly acidic, anoxic environment that may serve to degrade otherwise refractory bacilli.The structure and development of mycobacterial granulomas in mammals, especially those produced in response to Mycobacterium tuberculosis and BCG (attenuated M. bovis), have been described (Papadimitriou & Spector 1972, Ad...

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Section: Discussionmentioning

confidence: 99%

Ultrastructure of Mycobacterium marinum granuloma in striped bass Morone saxatilis

Gauthier

Vogelbein²,

Ottinger³

2004

Dis. Aquat. Org.

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“…Activated macrophages were reported to have an important role in the formation of CLC in several disease processes (48, 109). CLC and damaged eosinophils, along with their granules, have been observed within macrophages (27, 48, 109). It has been proposed that after the degranulation of eosinophils, CLC protein could be phagocytized into acidified membrane‐bound lysosomes (109).…”

Section: Non‐living Etiologic Agentsmentioning

confidence: 99%

Diagnosis, prognosis and decision‐making in the treatment of combined periodontal‐endodontic lesions

Rotstein

Simon²

2004

Periodontology 2000

150

102

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“…Their presence is most often associated with increased numbers of peripheral blood or tissue eosinophils in parasitic, allergic, neoplastic and inflammatory diseases . Activated macrophages are reported to have an important role in the formation of Charcot–Leyden crystals in several disease processes . Charcot–Leyden crystals and damaged eosinophils, along with their granules, have been observed within macrophages .…”

Section: Disease Relationshipsmentioning

confidence: 99%

“…Activated macrophages are reported to have an important role in the formation of Charcot–Leyden crystals in several disease processes . Charcot–Leyden crystals and damaged eosinophils, along with their granules, have been observed within macrophages . It has been proposed that after the degranulation of eosinophils, Charcot–Leyden crystal protein can be phagocytized into acidified membrane‐bound lysosomes .…”

Section: Disease Relationshipsmentioning

confidence: 99%

Interaction between endodontics and periodontics

Rotstein¹

2017

Periodontology 2000

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Endodontic-periodontal lesions present challenges to the clinician regarding diagnosis, treatment planning and prognosis. Etiologic factors, such as bacteria and viruses, as well as contributing factors, such as trauma, root resorptions, perforations, cracks and dental malformations, play an important role in the development and progression of such lesions. Treatment and prognosis of endodontic-periodontal lesions vary, depending on the etiology, pathogenesis and correct diagnosis of each specific condition. This chapter will appraise the interrelationship between endodontic and periodontal lesions and provide biological and clinical evidence for diagnosis, prognosis and decision-making in the treatment of these conditions.

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Identification of Charcot-Leyden Crystals by Electron Microscopy

Cited by 19 publications

References 23 publications

Ultrastructure of Mycobacterium marinum granuloma in striped bass Morone saxatilis

Ultrastructure of Mycobacterium marinum granuloma in striped bass Morone saxatilis

Diagnosis, prognosis and decision‐making in the treatment of combined periodontal‐endodontic lesions

Interaction between endodontics and periodontics

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