Identification of characteristic TRB V usage in HBV-associated HCC by using differential expression profiling analysis

Han, Yingxin; Liu, Xing; Wang, Yuqi; Wu, Xiaolei; Guan, Yanfang; Li, Hongmei; Chen, Xinchun; Zhou, Boping; Yuan, Qing; Ou, Ying; Wu, Renhua; Huang, Wanqiu; Wang, Yun; Zhang, Ming; Zhang, Yinxin; Zhu, Dongxing; Zhu, Hongmei; Yang, Ling; Yi, Xin; Huang, Chen; Huang, Jian

doi:10.1080/2162402x.2015.1021537

Cited by 36 publications

(36 citation statements)

References 44 publications

(22 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…By high throughput TCR sequencing of T‐cell repertoires, differential TCR signatures had been identified in primary biliary cirrhosis, primary sclerosing cholangitis, and alcoholic liver disease, which represented an imprint of distinctive antigenic repertoires in these chronic liver diseases 13. Similarly, significant differences of T‐cell repertoires among HCC, intrahepatic cholangiocarcinoma, and mixed hepatocellular and cholangiocellular carcinoma have also been demonstrated 14. Besides, the T‐cell repertoires in tumor and matched adjacent nontumor tissues from HBV‐associated HCC patients have also shown a significant difference, suggesting a distinct T cell immune microenvironment 15.…”

Section: Introductionmentioning

confidence: 99%

T cell receptor repertoire profiling predicts the prognosis of HBV‐associated hepatocellular carcinoma

et al. 2018

View full text Add to dashboard Cite

Tumor‐infiltrating T cell repertoire has been demonstrated to be closely associated with anti‐tumor immune response. However, the relationship between T cell repertoire in tumor tissue and prognosis has never been reported in Hepatocellular carcinoma (HCC). We performed the high‐throughput T cell receptor (TCR) sequencing to systematically characterize the infiltrating T cell repertoires of tumor and matched adjacent normal tissues from 23 HBV‐associated HCC patients. Significant differences on usage frequencies of some Vβ, Jβ, and Vβ‐Jβ paired genes have been found between the 2 groups of tissue samples, but no significant difference of TCR repertoire diversity could be found. Interestingly, the similarity of TCR repertoires between paired samples or the TNM stage alone could not be helpful to evaluate the prognosis of patients very well, but their combination could serve as an efficient prognostic indicator that the patients with early stage and high similarity showed a better prognosis. This is the first attempt to assess the potential value of TCR repertoire in HCC prognosis, and our findings could serve as a complement for the characterization of TCR repertoire in HCC.

show abstract

Section: Introductionmentioning

confidence: 99%

T cell receptor repertoire profiling predicts the prognosis of HBV‐associated hepatocellular carcinoma

et al. 2018

View full text Add to dashboard Cite

show abstract

Section: Reduced Clonal Diversity and Enhanced Clonal Expansion And Ementioning

confidence: 99%

“…A unique CDR3 clone whose reads account for .0.1% of total reads stands for a "highly expressed clone" (HEC-unique CDR3) (22). A lineage whose reads from all unique CDR3s account for .0.1% of the total reads represents a "highly expanded and expressed clone" (HEC-lineage) (22). The ratio of HEC-lineage numbers to total lineage numbers was referred to as HEC-lineage number rate, and so was the HEC-unique CDR3 number rate, both of which were used to reflect the degree of clonal expansion and expression.…”

Section: Reduced Clonal Diversity and Enhanced Clonal Expansion And Ementioning

confidence: 99%

Clonal Characteristics of Circulating B Lymphocyte Repertoire in Primary Biliary Cholangitis

Tan

Wang

Zhang

et al. 2016

The Journal of Immunology

Self Cite

View full text Add to dashboard Cite

Primary biliary cholangitis (PBC) is an autoimmune liver disease characterized by elevated serum anti-mitochondrial Ab and lymphocyte-mediated bile duct damage. This study was designed to reveal the clonal characteristics of B lymphocyte repertoire in patients with PBC to facilitate better understanding of its pathogenesis and better management of these patients. Using high-throughput sequencing of Ig genes, we analyzed the repertoire of circulating B lymphocytes in 43 patients with PBC, and 34 age- and gender-matched healthy controls. Compared with healthy controls, PBC patients showed 1) a gain of 14 new clones and a loss of 8 clones; 2) a significant clonal expansion and increased relative IgM abundance, which corresponded with the elevated serum IgM level; 3) a significant reduction of clonal diversity and somatic hypermutations in class-switched sequences, which suggested a general immunocompromised status; 4) the reduction of clonal diversity and enhancement of clonal expansion were more obvious at the cirrhotic stage; and 5) treatment with ursodeoxycholic acid could increase the clonal diversity and reduce clonal expansion of the IgM repertoire, with no obvious effect on the somatic hypermutation level. Our data suggest that PBC is a complex autoimmune disease process with evidence of B lymphocyte clonal gains and losses, Ag-dependent ogligoclonal expansion, and a generally compromised immune reserve. This new insight into the pathogenesis of PBC opens up the prospect of studying disease-relevant B cells to better diagnose and treat this devastating disease.

show abstract

“…[14][15][16] A recent investigation of HBV-associated HCC indicated that the highly expanded clone (HEC) ratio of TCRb CDR3 (TRB CDR3) was significantly different in liver cancer compared to healthy and hepatitis B patients. 17 The comparison of TRB CDR3 among three histological types of liver cancer samples revealed that the consistency between tumor tissues and adjacent non-tumor tissues was related to tumor malignancy. However, because HBV-associated HCC develops as a result of HBV infection, the immune microenvironments differ between tumor and adjacent non-tumor tissues, and the consequent TRB CDR3 diversity should be elucidated, respectively.…”

Section: Introductionmentioning

confidence: 99%

High-throughput T cell receptor sequencing reveals distinct repertoires between tumor and adjacent non-tumor tissues in HBV-associated HCC

Chen

Zhao

et al. 2016

OncoImmunology

View full text Add to dashboard Cite

T lymphocytes, which recognize antigen peptides through specific T cell receptors (TCRs), play an important role in the human adaptive immune response. TCR diversity is closely associated with host immune response and cancer prognosis. Although tumor-infiltrating T lymphocytes have implications for tumor prognosis, few studies have performed a detailed characterization of TCR diversity in both tumor and non-tumor tissues in hepatitis B virus (HBV)-associated hepatocellular carcinoma (HCC). Here, we performed high-throughput sequencing of the TCRb chain complementarity determining region 3 (CDR3) of liver-infiltrating T cells from 48 HBV-associated HCC patients. A significantly higher average number of CDR3 aa clonotypes (2259 vs. 1324, p < 0.001), and significantly higher TCR diversity (Gini coefficient, p < 0.001; Simpson index, p < 0.01; Shannon entropy, p < 0.001) were observed in tumor tissues compared with adjacent non-tumor tissues. The ratio of highly expanded clones (HECs) was significantly higher in non-tumor tissues than in tumor tissues when the HEC threshold was defined as 2% or greater (p < 0.05). Our analysis of the median Morisita-Horn index indicated weak TCR repertoire similarity between tumor and matched non-tumor tissues. The median number of shared clones in tumor tissue and matched non-tumor tissue from each patient was 360.5, representing 5.1-15.8% (10.6 § 0.4%) of all clones in each patient. We observed extensive heterogeneity of T lymphocytes in tumors and higher HEC ratios in adjacent non-tumor tissues of HCC patients. The differential T cell repertoires in tumor and non-tumor tissues suggest a distinct T cell immune microenvironment in patients with HBV-associated HCC.

show abstract

Identification of characteristic TRB V usage in HBV-associated HCC by using differential expression profiling analysis

Cited by 36 publications

References 44 publications

T cell receptor repertoire profiling predicts the prognosis of HBV‐associated hepatocellular carcinoma

T cell receptor repertoire profiling predicts the prognosis of HBV‐associated hepatocellular carcinoma

Clonal Characteristics of Circulating B Lymphocyte Repertoire in Primary Biliary Cholangitis

High-throughput T cell receptor sequencing reveals distinct repertoires between tumor and adjacent non-tumor tissues in HBV-associated HCC

Contact Info

Product

Resources

About