Identification of Cellular Voids in the Human Otic Capsule

Hansen, Lars Juul; Bloch, Sune Land; Sørensen, Mads Sølvsten

doi:10.1007/s10162-021-00810-6

Cited by 2 publications

(5 citation statements)

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“…Studies of the cellular network and irregularities of the human otic capsule have led to the discovery of osteocyte-depleted areas termed cellular voids (12). This study set out to quantify and map the distribution of cellular voids around the inner ear using unbiased stereology.…”

Section: Discussionmentioning

confidence: 99%

“…Because basic fuchsin is fluorescent, the voids appear as unstained islands in an otherwise continuous network of basic fuchsin-stained osteocytes against the green autofluorescence of bone matrix. Cellular voids were therefore identified using fluorescent light as previously described (12).…”

Section: Discussionmentioning

confidence: 99%

“…Stereological work was performed in the VIS 2017.2(7.0) software (Visiopharm, Hørsholm, Denmark). All slides were scanned in a meander pattern in blue, fluorescent light (450–490 nm; Zeiss filter set 09; band pass, 450–490; beam splitter, FT 510; emission, LP 515) at low magnification (4×/0.13) (12) to identify the cellular voids. A point grid was superimposed in systematic uniform random positions to estimate the size of the voids.…”

Section: Methodsmentioning

confidence: 99%

“…Osteocytes are connected via the lacunocanalicular network, which is vital for their nutritional supply and signal transduction (8)(9)(10). Histological studies and computer simulations have demonstrated how the connectivity of osteocytes may be disrupted locally to form so-called cellular voids (11,12). Such osteocyte depleted voids may interrupt the OPG-signal allowing focal pathological remodeling as seen in otosclerosis (13,14).…”

Section: Introductionmentioning

confidence: 99%

“…Basic fuchsin bulk-stained undecalcified temporal bones have been used to document the accumulation of dead osteocytes and microfractures around the inner ear (5)(6)(7). Recently, the identification of cellular voids in undecalcified human temporal bones was validated (12) (Fig. 1).…”

Section: Introductionmentioning

confidence: 99%

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The Spatial Distribution of Cellular Voids in the Human Otic Capsule: An Unbiased Quantification of Osteocyte-Depleted Areas

2022

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Objective: This study aimed to describe the spatial distribution of osteocyte-depleted areas, so-called cellular voids, in the human otic capsule and compare it with that of otosclerosis. Background: Systematic histological studies of the bony otic capsule have revealed an osteoprotegerin (OPG)-mediated inhibition of normal bone remodeling around the inner ear. The resulting accumulation of bony degeneration and dead osteocytes has been thoroughly documented, and the spatial distribution of dead osteocytes and matrix microcracks resembles that of the human ear disease otosclerosis. Clusters of dead osteocytes that may interfere with osteocyte connectivity and thereby the OPG signaling pathway have been described in human temporal bones. It is possible that these cellular voids create disruptions in the antiresorptive OPG signal that may give rise to local pathological remodeling.Methods: Recently, a method of detecting cellular voids was developed. This study uses unbiased stereology to document the spatial distribution of cellular voids in bulk-stained undecalcified human temporal bone. Results: Cellular voids accumulate around the inner ear and increase in number and size with age. Furthermore, cellular voids are more frequently found in the anterior and lateral regions of the otic capsule, which are known predilection sites of otosclerosis. Conclusion: This colocalization of cellular voids and otosclerosis suggests a causal relationship between focal degeneration and otosclerotic remodeling.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%