Identification of cell surface polypeptides encoded by human chromosomes 2 and 5 in human-mouse somatic cell hybrids

Abstract

Iodinated cell surface polypeptides of human-mouse somatic cell hybrids were analyzed by one-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis and high resolution two-dimensional gel electrophoresis in an effort to identify human gene products. Expression of two cell surface polypeptides was positively correlated with retention of specific human chromosomes: a polypeptide of molecular weight 250,000 and isoelectric point 8.3 with the human 5, and a polypeptide of molecular weight 250,000 and isoelectric point 7.0 with the human 2.

Report of the committee on the genetic constitution of chromosomes 5 and 6

“…They identified cell surface antigens in reduced rodent/human somatic cell hybrids by monoclonal anti bodies. Carlin (1983) found correlation between expression of a cell surface polypeptide (MW 250kd,pi 8.3, gene symbol PCH2) and retention of human chromosome 5 in human/mouse cell hybrids. In an in situ hybridization study with the cloned genes for C0L1A1 and C0L1A2, Retief et al (HGM8) noticed significant cross hybridization to other chromosomes than 17 and 7, including 5q23-31.…”