Identification of carbapenem‐resistant Acinetobacter baumannii clones using infrared spectroscopy

Abstract

In this work we assessed the discriminatory ability of Fourier‐transform Infrared Spectroscopy (FTIR) in 22 representative isolates from a collection of 318 carbapenem‐hydrolyzing class D β ‐lactamases (CHDL)‐producing Acinetobacter spp. (5 hospitals; 2001–2008) previously characterized by DNA‐based typing methods. FTIR spectra were acquired with a Bruker spectrometer and analyzed with support of several chemometric tools. The results showed that FTIR spectroscopy was able to distinguish the main CHDL‐producing Acinetobacter baumannii lineages causing infection in Portugal, the ST103 carrying blaOXA‐58, ST98 carrying blaOXA‐24/40and ST92 carrying blaOXA‐23. Moreover, this study revealed distinctive phenotypic features of A. baumannii lineages causing infections that might justify different epidemic potential. Spectroscopy may arise as a low cost and easily to perform alternative for typing A. baumannii isolates. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

An Overview of the Evolution of Infrared Spectroscopy Applied to Bacterial Typing

“…FTIRS and MALDI-TOF MS demonstrated a similar potential for the discrimination of two pathovars of X. oryzae [79] and Goodacre et al [91] concluded that FTIRS was better than pyrolysis mass spectrometry and dispersive Raman microscopy for the species discrimination of 59 Gram-positive and -negative bacteria. Regarding the subspecies level, the discrimination of an Acinetobacter collection belonging to four distinct sequence types [69,70,89] was better with FTIRS than MALDI-TOF MS in. In contrast, similar results were obtained with FTIRS and MALDI-TOF MS regarding the discrimination of Klebsiella oxytoca [87] and Campylobacter strains.…”

“…Species FTIRS -- [41] (Continued) Rathayibacter tritici Species FTIRS -- [41] Lactococcus cremoris Species FTIRS PLS, KPLS [42] Rhodococcus erythropolis Species FTIRS -- [38] Acinetobacter spp. Species FTIRS, FTIRS-ATR HCA, PCA, DFA, ANN [43][44][45] Acinetobacter baumannii Sequence type FTIRS-ATR PLSDA [69,70] Burkholderia cepacia complex Species FTIRS PLS, PCA, SIMCA, ANN [45,46] Ribopatterns FTIRS PCA, PLS-DA [46] Pseudomonas spp. Species FTIRS HCA, ANN, PCA, SIMCA [45,49,53,86,92] Stenotrophomonas maltophilia Species FTIRS PCA, ANN [45,55] Ralstonia pickettii Species FTIRS ANN [45] Achromobacter spp Species FTIRS ANN [45] Yersinia spp.…”

“…[61][62][63] FTIRS combined with appropriate chemometric methods were also able to discriminate several serotypes of Streptococcus pneumonia, [64] S. aureus, [65] and Y. enterocolitica [47] and three Legionella pneumophila serogroups. [66] E. coli [67,68] and A. baumannii [69,70] were successfully discriminated according to their sequence types by IR spectroscopy and chemometrics, E. faecium isolates according their PFGE type [71] and E. coli according to their MLVA profiles. [72] Several studies were also found in the literature aiming to discriminate specific bacterial lineages.…”

“…[44,87,88] On the other hand, FTIRS seems to have high potential for subspecies discrimination due to the information available in the IR spectra namely, several classes of biomolecules, including lipids, nucleic acids, and carbohydrates. [69,70,87,89] In 2004, Wenning et al [90] studied the influence of the incubation time and medium type for typing bacteria at the genus, species, and strain level: FTIRS and MALDI-TOF MS were compared. The higher percentage of correct class assignments were achieved with the mass spectroscopic technique.…”

IR and Raman Spectroscopy for Pathogen Detection

“…Emphasis was also placed on the detection, discrimination, classification, and identification of bacteria -especially foodborne pathogens -such as Bacillus [19,20], Brucella [21], cyanobacteria [22], E. coli [23,24], lactobacilli [25], Listeria [26,27], Mycobacteria [28], Streptococcus [29], Salmonella [30,31], Staphylococcus [32,33], and Yersinia [34,35]. Also nosocomial pathogens, such as the members of the Burkholderia cepacia complex [36][37][38] possible warfare agents [39], and even fungi [40] were investigated. Thereby, samples in different conditions (liquid, suspended, powdered, or dehydrated) could be measured thanks to a variety of sampling methods and techniques available to record the spectra.…”