Identification of cannabinoid receptors in cultures of rat cerebellar granule cells

Long-Term Administration of Δ9-Tetrahydrocannabinol Desensitizes CB1-, Adenosine A1-, and GABAB-Mediated Inhibition of Adenylyl Cyclase in Mouse Cerebellum

“…This study also examined the interaction between CB 1 , A 1 , and GABA B receptors in cerebellum. In agreement with previous studies (Wojcik et al, 1985;Pacheco et al, 1993), GABA B receptors exhibited less than additive inhibition of adenylyl cyclase with either CB 1 or adenosine A 1 receptors. A novel finding of the present study is that CB 1 and A 1 receptors produced less than additive regulation of both G protein and adenylyl cyclase activity.…”

“…The finding that activation of G proteins by a combination of receptors was closer to additive than was inhibition of adenylyl cyclase suggests that adenylyl cyclase is the limiting factor in this signal transduction pathway. A similar conclusion was reached in previous studies comparing G protein and adenylyl cyclase responses (Wojcik et al, 1985;Pacheco et al, 1993). The finding that cerebellar GABA B and CB 1 receptors produce additive activation of G proteins agrees with a previous report (Pacheco et al, 1993).…”

“…A similar conclusion was reached in previous studies comparing G protein and adenylyl cyclase responses (Wojcik et al, 1985;Pacheco et al, 1993). The finding that cerebellar GABA B and CB 1 receptors produce additive activation of G proteins agrees with a previous report (Pacheco et al, 1993). However, the finding that G protein activation by GABA B and A 1 receptors was less than additive disagrees with a previous study that reported complete additivity (Wojcik et al, 1985).…”

International Union of Basic and Clinical Pharmacology. LXXIX. Cannabinoid Receptors and Their Ligands: Beyond CB1and CB2

“…The GABA B antagonist phaclofen noncompetitively antagonized R-(ϩ)-WIN55212-stimulated [ 35 S]GTP␥S binding in hippocampal membranes, and a CB 1 antagonist competitively antagonized the response to 3-aminopropyl(methyl) phosphinic acid (SKF97541) (Cinar et al, 2008). Agoniststimulation of CB 1 and GABA B receptors, both endogenously expressed in cerebellar granule cells, resulted in a subadditive inhibition of adenylyl cyclase Pacheco et al, 1993). The response to agonists for ␣ 2 -adrenoceptor-or somatostatin receptor-mediated inhibition of N-type Ca 2ϩ channels was reduced by expression of exogenous CB 1 receptors in superior cervical ganglia neurons (Pan et al, 1998;Vásquez and Lewis, 1999).…”

The orphan receptor GPR55 is a novel cannabinoid receptor

“…Thus, cannabinoid receptors and endocannabinoids are physiologically or pathophysiologically relevant in a great diversity of tissues and organs like the CNS and cardiovascular, reproductive, endocrine, immune and gastrointestinal systems. Particularly, the CNS and its hypothalamic appetite-regulating control system have attracted much attention over the last ten years and endocannabinoids have classically been shown to play a role in the physiological regulation of food intake (Sofia and Knobloch, 1976;Anderson-Baker et al, 1979;Pacheco et al, 1993;Berry and Mechoulam, 2002;Fride, 2002), effects that are inhibited by the non-endogenous…”