Identification of canine circulating miRNAs as tumor biospecific markers using Next-Generation Sequencing and Q-RT-PCR

Agarwal, Payal; Crepps, Melissa P.; Stahr, Natalie; Kretzschmar, Will P; Harris, H; Prasad, Nripesh; Levy, Shawn; Smith, B. F.

doi:10.1016/j.bbrep.2021.101106

Cited by 11 publications

(11 citation statements)

References 32 publications

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“…ΔCt values for each sample were calculated by normalizing to beta-actin as the normalization control. ΔΔCt values were calculated using the mean of ΔCt values of all samples as a reference control [ 26 ]. Finally, the relative fold CAR expression for each sample was calculated using the formula −2 −(∆∆Ct) .…”

Section: Methodsmentioning

confidence: 99%

Characterization of Canine Adenovirus Type 2 Virus Infection Pattern in Canine and Human Cell Lines

Hogans

Kretzschmar

Higgins

et al. 2022

Advances in Virology

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Canine adenovirus type 2 (CAV2) is a nonhuman adenovirus with a known ability to infect human and canine cells. The cell surface receptors involved in CAV2 transduction are still unknown. Identification of these would provide valuable information to develop enhanced gene delivery tools and better understand CAV2 biology. CAV2 is erroneously grouped with Ad5 based on the knowledge that CAV2 may transduce using CAR. Therefore, we have evaluated CAV2 and Ad5 (CAV2GFP, Ad5G/L) infection patterns in various canine and human cell lines to determine their different tropisms. Our research demonstrates that CAV2 can successfully infect cells that Ad5 does not infect, and CAV2 infections do not correlate with CAR expression. CAV2 can infect cells that have a low or minimal expression of CAR. Our data suggest that CAV2 transduction is not dependent on the CAR receptor, and thus, it is crucial to find novel CAV2 receptors.

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Section: Methodsmentioning

confidence: 99%

Characterization of Canine Adenovirus Type 2 Virus Infection Pattern in Canine and Human Cell Lines

Hogans

Kretzschmar

Higgins

et al. 2022

Advances in Virology

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“…In the study by Agarwal et al, thirteen cancer cell lines (osteosarcoma, melanoma, breast tumor etc.) were sequenced via next-generation sequencing (NGS) and six miRNAs were identified, which were then studied and analyzed using Q-RT-PCR [ 129 ]. Cancer cells release circulatory miRNAs into the exosomal fraction, [ 130 ] and Cfa-miR-9 demonstrated consistent elevation in both NGS and PCR analyses, suggesting its potential as a promising diagnostic miRNA.…”

Section: Exosome-mediated Interactions Of Brain Tumor Cells With Stromamentioning

confidence: 99%

“…Cancer cells release circulatory miRNAs into the exosomal fraction, [ 130 ] and Cfa-miR-9 demonstrated consistent elevation in both NGS and PCR analyses, suggesting its potential as a promising diagnostic miRNA. The findings of this study propose the feasibility of identifying a pan-tumor specific miRNA through NGS and validating it via Q-RT-PCR, offering the potential for enhanced diagnostic efficiency [ 129 ].…”

Section: Exosome-mediated Interactions Of Brain Tumor Cells With Stromamentioning

confidence: 99%

Exosomes as Novel Diagnostic Biomarkers and Therapeutic Tools in Gliomas

Skouras

Gargalionis

Piperi

2023

IJMS

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Exosomes constitute small extracellular vesicles that contain lipids, proteins, nucleic acids, and glycoconjugates from the secreted cells and are capable of transmitting signals between cells and coordinating cellular communication. By this means, they are ultimately involved in physiology and disease, including development, homeostasis, and immune system regulation, as well as contributing to tumor progression and neurodegenerative diseases pathology. Recent studies have shown that gliomas secrete a panel of exosomes which have been associated with cell invasion and migration, tumor immune tolerance, potential for malignant transformation, neovascularization, and resistance to treatment. Exosomes have therefore emerged as intercellular communicators, which mediate the tumor–microenvironment interactions and exosome-regulated glioma cell stemness and angiogenesis. They may induce tumor proliferation and malignancy in normal cells by carrying pro-migratory modulators from cancer cells as well as many different molecular cancer modifiers, such as oncogenic transcripts, miRNAs, mutant oncoproteins, etc., which promote the communication of cancer cells with the surrounding stromal cells and provide valuable information on the molecular profile of the existing tumor. Moreover, engineered exosomes can provide an alternative system for drug delivery and enable efficient treatment. In the present review, we discuss the latest findings regarding the role of exosomes in glioma pathogenesis, their utility in non-invasive diagnosis, and potential applications to treatment.

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“…Five of the eight novel microRNAs were present more abundantly in the circulating exosomes of patients with osteosarcoma than in controls, indicating the role of these eight novel microRNAs in osteosarcoma diagnosis [ 101 ]. Agarwal et al used next-generation sequencing and Q-RT-PCR to show that the upregulated canine exosomal Cfa-miR-9 could act as a bispecific osteosarcoma markers [ 102 ]. What’s more, Yoshida and colleagues reported that miR-25-3p, which was expressed at a high level in tumor-derived exosomes, exhibited clinicopathological relevance and could potentially serve as a noninvasive biomarker in osteosarcoma [ 44 , 103 ].…”

Section: Role Of Evs In the Diagnosis And Therapeutics Of Osteosarcomamentioning

confidence: 99%

Extracellular vesicles in tumorigenesis, metastasis, chemotherapy resistance and intercellular communication in osteosarcoma

Liao

Qian

et al. 2023

Bioengineered

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Osteosarcoma, the most common primary malignant tumor of bone, occurs frequently in teenagers and is the third most common type of cancer among children and adolescents. Osteosarcoma originates from malignant mesenchymal stem cells. Fibroblasts, osteoblasts, osteoclasts, and immune cells, among other cell types, are present in the osteosarcoma microenvironment and work in conjunction to regulate osteosarcoma tumorigenesis. Extracellular vesicles (EVs) are spherical bilayer-membrane vesicles released by cells into extracellular spaces and body fluids, including plasma and synovial fluid. EV cargos contain various biomolecules, such as DNA, mRNAs, non-coding RNAs, proteins, lipids, and metabolites. EVs mediate intercellular communication by delivering these bioactive molecules to recipient cells and play a critical role in maintaining cellular homeostasis and promoting pathological progression. A growing body of evidence indicates that EVs play an important role in osteosarcoma tumorigenesis. In this review, we discuss existing knowledge on the important roles of tumor-derived or microenvironment-derived EVs in tumorigenesis, development, metastasis, drug resistance, and communication with the osteosarcoma microenvironment. We propose the potential of EVs in osteosarcoma diagnosis and treatment. Highlights Extracellular vehicles play crucial function in osteosarcoma tumorigenesis. Extracellular vehicles mediated the intercellular communication of osteosarcoma cells with other types cells in tumor microenvironment. Extracellular vehicles have potential utility in osteosarcoma diagnosis and treatment.

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Identification of canine circulating miRNAs as tumor biospecific markers using Next-Generation Sequencing and Q-RT-PCR

Cited by 11 publications

References 32 publications

Characterization of Canine Adenovirus Type 2 Virus Infection Pattern in Canine and Human Cell Lines

Characterization of Canine Adenovirus Type 2 Virus Infection Pattern in Canine and Human Cell Lines

Exosomes as Novel Diagnostic Biomarkers and Therapeutic Tools in Gliomas

Extracellular vesicles in tumorigenesis, metastasis, chemotherapy resistance and intercellular communication in osteosarcoma

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