Identification of candidate olfactory genes in cicada Subpsaltria yangi by antennal transcriptome analysis

Qi, Mengmeng; Wei, Songshan

doi:10.1016/j.cbd.2018.08.001

Cited by 6 publications

(5 citation statements)

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“…Herbivorous insects show variability in their ability to use plants, which is based on differences in preference, ingestion speed, digestion efficiency, and detoxification [26, 27]. Olfaction in antennae is essential for regulating insect behaviors such as host preference and oviposition site selection [28]. Many olfactory-related proteins are responsible for perceiving plant volatiles and mediating host selection of phytophagous insects [29, 30].…”

Section: Introductionmentioning

confidence: 99%

“…Many olfactory-related proteins are responsible for perceiving plant volatiles and mediating host selection of phytophagous insects [29, 30]. Recently, Qi et al [28] conducted transcriptome analysis based on RNA-Seq data from antennae of S. yangi to better understand the olfactory mechanisms in S. yangi which has a very narrow host range far fewer than diets of most other cicadas. They identified a number of olfactory genes of S. yangi , which provide direct evidence for future research of the olfactory system of S. yangi at the molecular level and provide information for elucidation of the molecular mechanisms and evolution of chemosensation in sap-sucking insects.…”

Section: Introductionmentioning

confidence: 99%

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De novo comparative transcriptome analysis of a rare cicada, with identification of candidate genes related to adaptation to a novel host plant and drier habitats

Hou

2019

BMC Genomics

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Background: Although the importance of host plant chemistry in plant-insect interactions is widely recognized, our understanding about the genetic basis underlying the relationship between changes in midgut proteins and adaptation of plant-feeding insects to novel host plants and habitats is very limited. To address this knowledge gap, the transcriptional profiles of midguts among three populations of the cicada Subpsaltria yangi Chen were compared. Among which, the Hancheng (HC) and Fengxiang (FX) populations occurring in the Loess Plateau feed on Ziziphus jujuba Mill. var. spinosa (Bunge) Hu ex H. F. Chow, while the population occurring in a much drier habitat in the Helan (HL) Mountains is locally specialized on a chemically divergent plant, Ephedra lepidosperma C. Y. Cheng. Results: Based on comparative analysis, 1826 (HL vs HC) differentially expressed genes (DEGs) and 723 DEGs (HL vs FX) were identified between the populations utilizing different host plants, including 20, 36, 2, 5 and 2 genes related to digestion, detoxification, oxidation-reduction, stress response and water-deprivation response, respectively, and 35 genes presumably associated with osmoregulation. However, only 183 DEGs were identified between the HC and FX populations, including two genes related to detoxification, two genes related to stress response, and one gene presumably associated with osmoregulation. These results suggest that the weakest expression differences were between the populations utilizing the same host plant and occurring in the closest habitats, which may help explain the metabolic mechanism of adaptation in S. yangi populations to novel host plants and new niches. Conclusions: The observed differences in gene expression among S. yangi populations are consistent with the hypothesis that the host plant shift and habitat adaptation in the HL population was facilitated by differential regulation of genes related to digestion, detoxification, oxidation-reduction, stress response, water-deprivation response and osmoregulation. The results may inform future studies on the molecular mechanisms underlying the relationship between changes in midgut proteins and adaptation of herbivorous insects to novel host plants and new niches.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

De novo comparative transcriptome analysis of a rare cicada, with identification of candidate genes related to adaptation to a novel host plant and drier habitats

Hou

2019

BMC Genomics

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“…Transcriptome-based tissue-specific comparative analysis is a powerful research technique since patterns of gene expression are likely to vary among disparate tissues, organs, or even different regions of the same organ concerning their respective functions [ 39 ]. Correlations between the transcriptomes in many tissues or organs of animals (e.g., antennae, salivary glands, midgut, midgut ‘appendices’, hepatopancreas, venom gland, gill) and its respective physiological functions have been reported [ 40 – 45 ]. Similar studies have been carried out for the MTs of a few insect species, but these studies have been limited in viewing the MTs as a whole organ [ 3 , 5 , 10 , 12 , 22 , 25 , 46 – 50 ].…”

Section: Discussionmentioning

confidence: 99%

Gene expression profiles in Malpighian tubules of the vector leafhopper Psammotettix striatus (L.) revealed regional functional diversity and heterogeneity

Yuan

2022

BMC Genomics

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Background Many leafhoppers are known as pests and disease vectors of economically important plants. Previous studies of the physiological functions of vector leafhoppers have mainly focused on the salivary glands and the alimentary tract that are deemed to be associated with digestion, host defense and phytoplasma and/or virus transmission. By contrast, the significance of Malpighian tubules (MTs) is less studied. To clarify the physiological function of MTs of the vector leafhopper Psammotettix striatus that transmits phytoplasma triggering the wheat blue dwarf disease, we performed a transcriptome study on P. striatus MTs and compared gene expression profiles among different anatomical regions in the tubules (i.e., MT1+2, the anterior segment together with the sub-anterior segment; MT3, the inflated segment; and MT4, the distal segment). Results Transcriptome of P. striatus MTs generate a total of 42,815 high-quality unigenes, among which highly expressed unigenes are mainly involved in organic solute transport, detoxification and immunity in addition to osmoregulation. Region-specific comparative analyses reveal that all these MT regions have functions in osmoregulation, organic solute transport and detoxification, but each region targets different substrates. Differential expression and regional enrichment of immunity-related effector activities and molecules involved in phagocytosis and the biosynthesis of antimicrobial peptides among different regions indicate that MT1+2 and MT4 have the ability to eliminate the invading pathogens. However, in MT3 which secrets brochosomes to the integument and eggs as physical barriers, disulfide-isomerase, acidic ribosomal protein P and many other unigenes were highly expressed, which can be attractive candidate genes for future studies of the biosynthesis and the origin of brochosomes. Conclusions Psammotettix striatus MTs perform multiple physiological functions as versatile organs than just excretory organs with osmoregulatory function. Heterogeneity of physiological functions among different MT regions is related to organic solute transport, detoxification, immunity and brochosome biosynthesis in addition to osmoregulation, and each region targets different substrates. These functions may be helpful for P. striatus to resist pathogens from habitats and to utilize a wider range of host plants, which may assist the transmission and spread of phytoplasmas. The results provide potential molecular targets for the exploit of chemical and/or gene-silencing insecticides.

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“…Olfaction is essential for insects to identify host substrates, mates and oviposition sites (Qi et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Identification of the Differences in Olfactory System Between Male and Female Oriental Tobacco Budworm Helicoverpa assulta 

Miao

et al. 2020

Preprint

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Background: The olfactory system of insects facilitates their search for host and mates, hence it plays an essential role for insect survival and reproduction. Insects recognize odor substances through olfactory neurons and olfactory genes. Previous studies showed that there are significant sex-specific differences in how insects identify odorant substances, especially sex pheromones. However, whether the sex-specific recognition of odorant substances is caused by differences in the expression of olfaction-related genes between males and females remains unclear. Results: In order to clarify this problem, the whole transcriptome sequence of the adult Helicoverpa assulta, an important agricultural pest of tobacco and other Solanaceae plants, was obtained using PacBio sequencing. RNA-seq analysis showed that there were 27 odorant binding proteins (OBPs), 24 chemosensory proteins (CSPs), 4 pheromone-binding proteins (PBPs), 68 odorant receptors (OR) and 2 sensory neuron membrane proteins (SNMPs) genes that were expressed in the antennae of male and female H. assulta. Females had significantly higher expression of GOBP1-like, OBP, OBP3, PBP3 and SNMP1 than males, while males had significantly higher expression of GOBP1, OBP7, OBP13, PBP2 and SNMP2. Conclusions: Our findings improve our understanding of olfactory genes in H. assulta, and can be used to further study pheromone identification, mate search, and sex differences in an insect’s ability to search for hosts. These results improve our understanding of mate search and host differentiation in H. assulta.

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Identification of candidate olfactory genes in cicada Subpsaltria yangi by antennal transcriptome analysis

Cited by 6 publications

References 51 publications

De novo comparative transcriptome analysis of a rare cicada, with identification of candidate genes related to adaptation to a novel host plant and drier habitats

De novo comparative transcriptome analysis of a rare cicada, with identification of candidate genes related to adaptation to a novel host plant and drier habitats

Gene expression profiles in Malpighian tubules of the vector leafhopper Psammotettix striatus (L.) revealed regional functional diversity and heterogeneity

Identification of the Differences in Olfactory System Between Male and Female Oriental Tobacco Budworm Helicoverpa assulta

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Identification of candidate olfactory genes in cicada Subpsaltria yangi by antennal transcriptome analysis

Cited by 6 publications

References 51 publications

De novo comparative transcriptome analysis of a rare cicada, with identification of candidate genes related to adaptation to a novel host plant and drier habitats

De novo comparative transcriptome analysis of a rare cicada, with identification of candidate genes related to adaptation to a novel host plant and drier habitats

Gene expression profiles in Malpighian tubules of the vector leafhopper Psammotettix striatus (L.) revealed regional functional diversity and heterogeneity

Identification of the Differences in Olfactory System Between Male and Female Oriental Tobacco Budworm Helicoverpa assulta&nbsp;

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Identification of the Differences in Olfactory System Between Male and Female Oriental Tobacco Budworm Helicoverpa assulta