Identification of candidate biomarkers correlated with pathogenesis of postoperative peritoneal adhesion by using microarray analysis

Bian, Yaoyao; Yang, Lili; Yan, Yan; Zhao, Min; Chen, Yanqi; Zhou, Yaqi; Wang, Zixin; Li, Wenlin; Zeng, Li

doi:10.4251/wjgo.v12.i1.54

Cited by 9 publications

(8 citation statements)

References 24 publications

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“…The degree of adhesion was determined using ve-stage adhesion score system [13][28] [29] by two independent investigators in a blinded way. This scoring system included ve stages adhesion scores range from 0 to 4.…”

Section: Adhesion Grading and Evaluationmentioning

confidence: 99%

“…And a signi cant increase of Th2 cells was found in the adhesion area of a rodent experiment [8] . Previously, we reported that the exact roles of TLR4/MyD88/NF-κB signaling pathway in the pathogenesis of PPA based on the microarray analysis [13] . Herein, it might be a bene cial way to nd new approach to regulate Th1/Th2 balance through TLR4/MyD88/NF-κB regulatory chain.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Ligustrazine Nanoparticles on Th1/Th2 Balance by TLR4/MyD88/NF-κB Pathway in Rats with Postoperative Peritoneal Adhesion

Yang

Lian

Zhang³

et al. 2020

Preprint

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Background Postoperative peritoneal adhesion (PPA) is regarded as fibrous bands connecting both injured abdominal wall and organs or adjacent tissues and associated with T helper (Th)1 and Th2 differentiation. However, the critical role of immunopathogenesis of adhesion formation was precisely unknown. Previously we demonstrated that ligustrazine have positive effects on adhesion formation. And microarray analysis revealed TLR4/MyD88/NF-κB pathway as an important underlying regulatory mechanism in the onset and development of PPA. To further explore the possible mechanism of PPA and enhance the specificity of ligustrazine delivery to injured sites of rodent models, a new agent polylactic acid (PLA) nanoparticles loaded with ligustrazine, that is, ligustrazine nanoparticles (LN) were applied. Methods Twenty-four SD rats were randomly divided into sham, model, LN and sodium hyaluronate (SH) groups. The structure of LN, including entrapment efficiency (EE) and loading capacity (LC), and in vitro drug release were calculated. Adhesions were scored and the masson’s trichrome staining was used to determine the collagen deposition. The expression of TLR4, MyD88 and NF-κB were measured by qPCR, Immunohistochemistry and western blot assay. Moreover, Th1-related cytokines (IFN-γ, IL-12), Th2-related cytokines (IL-4, IL-6) in the cecum tissue and serum were conducted by ELISA. Results LN had good EE, LC and control-release delivery characters with fairly uniform diameter and spherical morphology. It could effectively prevent adhesion formation after surgery. Besides, it could reduce collagen fibers accumulation, downregulate the expression levels of TLR4, MyD88 and NF-κB, and maintain Th1/Th2 balance. Conclusions Ligustrazine nanoparticles had effective effects on Th1/Th2 balance by regualtingTLR4/MyD88/NF-κB pathway in PPA rats. It may be served as a promising therapy on postoperative adhesion formation.

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Section: Adhesion Grading and Evaluationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Ligustrazine Nanoparticles on Th1/Th2 Balance by TLR4/MyD88/NF-κB Pathway in Rats with Postoperative Peritoneal Adhesion

Yang

Lian

Zhang³

et al. 2020

Preprint

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“…The degree of adhesion was determined using a five-stage adhesion score system [ 13 , 24 , 25 ] by two independent investigators in a blinded way. This scoring system included five stages adhesion scores ranging from 0 to 4.…”

Section: Methodsmentioning

confidence: 99%

“…And a significant increase of Th2 cells was found in the adhesion area of a rodent experiment [ 8 ]. Previously, we reported that the exact roles of the TLR4/MyD88/NF-κB signaling pathway in the pathogenesis of PPA based on the microarray analysis [ 13 ]. Herein, it might be a beneficial way to find a new approach to regulate Th1/Th2 balance through TLR4/MyD88/NF-κB regulatory chain.…”

Section: Introductionmentioning

confidence: 99%

Effect of ligustrazine nanoparticles on Th1/Th2 balance by TLR4/MyD88/NF-κB pathway in rats with postoperative peritoneal adhesion

Yang

Lian

Zhang³

et al. 2021

BMC Surg

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Background Postoperative peritoneal adhesion (PPA) is regarded as fibrous bands connecting both injured abdominal wall and organs or adjacent tissues. It is associated with T helper (Th)1 and Th2 differentiation. However, the critical role of the immunopathogenesis of adhesion formation was precisely unknown. The aim of this study was to investigate the effect of a new agent polylactic acid (PLA) nanoparticles loaded with ligustrazine, that is, ligustrazine nanoparticles (LN) on PPA and identify the potential mechanism. Methods Twenty-four Sprague–Dawley rats were randomly divided into the sham, model, LN, and sodium hyaluronate (SH) groups. The structure of LN, including entrapment efficiency (EE) and loading capacity (LC), and in vitro drug release were calculated. Adhesions were scored and the Masson's trichrome staining was used to determine the collagen deposition. The expressions of TLR4, MyD88, and NF-κB were measured by qRT-PCR, immunohistochemistry, and western blot assay. Moreover, Th1-related cytokines (IFN-γ, IL-12), Th2-related cytokines (IL-4, IL-6) in the cecum tissue and serum were conducted by ELISA. Results LN had good EE, LC, and control-release delivery characters with fairly uniform diameter and spherical morphology. It could effectively prevent adhesion formation after surgery. Besides, it could reduce collagen fibers accumulation, downregulate the expression levels of TLR4, MyD88, and NF-κB, and maintain Th1/Th2 balance. Conclusions Ligustrazine nanoparticles had effective effects on Th1/Th2 balance by regulating TLR4/MyD88/NF-κB pathway in PPA rats. It may be served as a promising therapy on postoperative adhesion formation.

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“…Although it is still unknown which cells sense the initial insult that leads to a pathological phenotype downstream, a few studies have elucidated the gene expression changes implicated in adhesion formation [ 39 , 167 , 168 , 169 , 170 ]. Micro-array analysis has identified several hub genes, such as TNF and IL6, to be significant biomarkers in the pathogenesis of post-operative peritoneal adhesions [ 171 ]. Ongoing studies are examining the potential genetic predispositions to post-operative adhesion development [ 169 ].…”

Section: Mechanisms Driving Post-surgical Adhesion Formationmentioning

confidence: 99%

Post-Operative Adhesions: A Comprehensive Review of Mechanisms

et al. 2021

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Post-surgical adhesions are common in almost all surgical areas and are associated with significant rates of morbidity, mortality, and increased healthcare costs, especially when a patient requires repeat operative interventions. Many groups have studied the mechanisms driving post-surgical adhesion formation. Despite continued advancements, we are yet to identify a prevailing mechanism. It is highly likely that post-operative adhesions have a multifactorial etiology. This complex pathophysiology, coupled with our incomplete understanding of the underlying pathways, has resulted in therapeutic options that have failed to demonstrate safety and efficacy on a consistent basis. The translation of findings from basic and preclinical research into robust clinical trials has also remained elusive. Herein, we present and contextualize the latest findings surrounding mechanisms that have been implicated in post-surgical adhesion formation.

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Identification of candidate biomarkers correlated with pathogenesis of postoperative peritoneal adhesion by using microarray analysis

Cited by 9 publications

References 24 publications

Effect of Ligustrazine Nanoparticles on Th1/Th2 Balance by TLR4/MyD88/NF-κB Pathway in Rats with Postoperative Peritoneal Adhesion

Effect of Ligustrazine Nanoparticles on Th1/Th2 Balance by TLR4/MyD88/NF-κB Pathway in Rats with Postoperative Peritoneal Adhesion

Effect of ligustrazine nanoparticles on Th1/Th2 balance by TLR4/MyD88/NF-κB pathway in rats with postoperative peritoneal adhesion

Post-Operative Adhesions: A Comprehensive Review of Mechanisms

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