Identification of Candida Species in the Clinical Laboratory: A Review of Conventional, Commercial and Molecular Techniques

Sudhan, Shashi S.; Sharma, Preeti; Sharma, Maneesh; Shrivastava, Divya Rishi

doi:10.21276/ijmrp.2016.2.6.001

Cited by 7 publications

(9 citation statements)

References 10 publications

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“…[ 6 ] Emergence of drug-resistant strains of Candida has highlighted the need for reliable methods of candidial isolation and identification. [ 16 ] Thus, the above study was undertaken to comparatively evaluate the phenotypic variation of Candida species in patients of OLP and OLL with healthy individuals using indirect microscopic examination.…”

Section: Discussionmentioning

confidence: 99%

“…On performing GT test for differentiation into C. albicans and non C. albicans species,[ 16 ] the frequency of C. albicans was higher in control group and non C. albicans species in study (OLP + OLL) group. To the best of our knowledge, no other studies show an increased number of non C. albicans in OLP and OLL as compared to healthy controls.…”

Section: Discussionmentioning

confidence: 99%

“…The formation of germ tubes is influenced by various environmental factors (temperature, concentration of inoculum, composition of the medium, pH and bacterial contamination) which may account for false readings. [ 16 ] One possible concern about this technique is that 95% of C. albicans isolates or some non C. albicans ( C. dubliniensis , C. tropicalis and C. parapsilosis) can also generate germ tube or pseudohyphae in serum, leading to misidentification if only perfunctory tests are performed. [ 15 ]…”

Section: Discussionmentioning

confidence: 99%

“…Sugar assimilation was depicted as a positive or a negative reaction for various sugars, as seen in [ Table 1b ]. [ 16 ]…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Comparative evaluation of prevalence and phenotypic variations of Candida species in patients of oral lichen planus and oral lichenoid lesions with healthy individuals - A prospective microbiological study

Sarkate

Pathak

Patel

et al. 2022

J Oral Maxillofac Pathol

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Sugar assimilation was depicted as a positive or a negative reaction for various sugars, as seen in [ Table 1b ]. [ 16 ]…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Comparative evaluation of prevalence and phenotypic variations of Candida species in patients of oral lichen planus and oral lichenoid lesions with healthy individuals - A prospective microbiological study

Sarkate

Pathak

Patel

et al. 2022

J Oral Maxillofac Pathol

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“…The API 20 C AUX system (Bio-Merieux, France) was also used for differentiation of Candida spp. The cupules turbidity was identified after incubation at 37°C for 24−72 h by using the analytical profile index and reading them as compared to the growth control (0 cupule) 18 .…”

Section: Api 20 C Aux Systemmentioning

confidence: 99%

Species Identification of Candida Isolates from Critically Ill Patients by Rapid Commercial and Genotypic Methods

Elnagar

Sultan

Ahmed

2023

Egyptian Journal of Medical Microbiology

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Background: Rapid, reliable Candida species (spp.) identification is crucial because it enables prompt and effective antifungal treatment. Unfortunately this is difficult due to a lack of knowledge, adequate reagents, and equipments, particularly in countries with limited resources. Objectives: The aim of this study was to assess the effectiveness of rapid commercial methods in identifying Candida spp. isolated from critically ill patients. Methodology: Various clinical specimens were collected from critically ill patients admitted to the Intensive Care Units of Mansoura University Hospitals, and processed by numerous mycological techniques. Gram stained, and lactophenol cotton blue stained films, as well, germ tube test, and corn meal agar with 1% Tween 80 were used to identify the isolated Candida spp. Three commercial methods were used for spp. identification; CHROMagar TM Candida medium, API 20 C AUX and Vitek-2 Compact automated system. In addition, all Candida isolates were subjected to species-specific multiplex PCR as the reference method. Results: A total of 52 Candida strains were identified where C. albicans was the predominant spp. (53.8%) followed by C. parapsilolsis (17.3%), C. glabrata (13.5%), C. dubliniensis (5.8%), C. tropicalis (5.8%), C. auris (1.9%) and C. krusei (1.9%). Both CHROMagar TM Candida medium and Vitek-2 Compact automated system exhibited high sensitivity and specificity rates in identification of various Candida spp. as compared to multiplex PCR. Conclusion: This study demonstrated a good performance of both CHROMagar TM Candida medium and Vitek-2 Compact automated system; however, CHROMagar TM Candida medium may be an easier and more cost-effective choice to identify Candida spp., mainly in areas with restricted resources.

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A critical review on anticandidal properties of marine plants

Priyanka,

Rajaram

2023

Regional Studies in Marine Science

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Identification of Candida Species in the Clinical Laboratory: A Review of Conventional, Commercial and Molecular Techniques

Cited by 7 publications

References 10 publications

Comparative evaluation of prevalence and phenotypic variations of Candida species in patients of oral lichen planus and oral lichenoid lesions with healthy individuals - A prospective microbiological study

Comparative evaluation of prevalence and phenotypic variations of Candida species in patients of oral lichen planus and oral lichenoid lesions with healthy individuals - A prospective microbiological study

Species Identification of Candida Isolates from Critically Ill Patients by Rapid Commercial and Genotypic Methods

A critical review on anticandidal properties of marine plants

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