Identification of Cancer Stem Cells Derived From a Canine Lung Adenocarcinoma Cell Line

Nemoto, Yasuhiro; Maruo, Takuya; Sato, T.; Deguchi, Takao; Ito, Takahiro; Sugiyama, Hiroki; Ishikawa, Takefumi; Madarame, Hiroo; Watanabe, Tetsuya; Shida, Takuo; Sahara, Hiroeki

doi:10.1177/0300985810396106

Cited by 27 publications

(22 citation statements)

References 22 publications

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“…CLAC was used in this study . Adherent CLAC was cultured in Dulbecco's Modified Eagle's Medium (D'MEM) medium (Sigma–Aldrich, St. Louis, MO, USA), supplemented with 10% foetal bovine serum (FBS), MEM non‐essential amino acids (Life technologies, Carlsbad, CA, USA), 200 units mL −1 penicillin (Life technologies), 200 mg mL −1 streptomycin (Life technologies) and 2 mM l ‐glutamine (Life technologies) and maintained in a humidified atmosphere with 5% CO 2 at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

“…The existence of canine CSCs/CICs have been reported in osteosarcoma, glioblastoma and mammary gland tumour . We previously established the canine lung adenocarcinoma cell (CLAC) line derived from primary canine lung adenocarcinoma and identified subpopulation exhibiting CSCs/CICs‐like properties from CLAC using the Hoechst 33342 dye‐based side population technique . The CSCs/CICs derived from CLAC was included at the ration of 0.075% and possess stemness property such as high tumourigenesis in vivo .…”

Section: Introductionmentioning

confidence: 99%

“…Various studies have been performed to characterize human CSCs/CICs, and several cell surface proteins and transcription factors, such as CD44, CD133, Sox2 and Oct‐4, have been identified as CSCs/CICs maker. It have been also reported that CSCs/CICs display high drug efflux capacity due to overexpression of Adenosine Triphosphate (ATP)‐binding cassette (ABC) transporters …”

Section: Introductionmentioning

confidence: 99%

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Radioresistance of cancer stem‐like cell derived from canine tumours

Tanabe

Deguchi

Sato

et al. 2014

Vet Comparative Oncology

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Cancer stem-like cells (CSCs)/cancer-initiating cells (CICs) are a small subpopulation of cancer cells that are responsible for the initiation, recurrence and metastasis of cancer. We previously demonstrated that, using the Hoechst 33342 dye-based side population technique, CSCs/CICs in canine lung adenocarcinoma cell line exist. In this study, as CSCs/CICs are known to form spheres in anchorage-independent environment in vitro, we evaluated the stemness of spheroid cells derived from canine lung adenocarcinoma and osteosarcoma cells by expression of stemness markers, and investigated radioresistance. Spheroid cells showed greater expression of stemness markers Oct-4 and CD133 gene than those of adherent-cultured cells. In nude mouse xenograft models, spheroid cells showed higher tumourigenic ability than adherent-cultured cells. In addition, spheroid cells showed significantly resistant against radioactivity as compared with adherent-cultured cells. These results suggest that spheroid cells could possess stemness and provide a CSCs/CICs research tool to investigate CSCs/CICs of canine tumour cells.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Radioresistance of cancer stem‐like cell derived from canine tumours

Tanabe

Deguchi

Sato

et al. 2014

Vet Comparative Oncology

Self Cite

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“…CSCs have been identified in many human cancers, including skin, breast, liver, lung, and so on [1], [12], [14], [15]. Within a tumor, CSCs, which constitute a small portion of neoplastic cells, are defined by their capacity to produce new tumors.…”

Section: Introductionmentioning

confidence: 99%

P38/NF-κB/Snail Pathway Is Involved in Caffeic Acid-Induced Inhibition of Cancer Stem Cells-Like Properties and Migratory Capacity in Malignant Human Keratinocyte

Yang

Wang

et al. 2013

PLoS ONE

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BackgroundSkin cancer is the most common cancer throughout the world. The epithelial-mesenchymal transition (EMT) and the acquisition of cancer stem cells (CSCs)-like properties emerge as critical steps in the metastasis of human skin cancers. Caffeic acid (CaA) exerts anticarcinogenic effects. However, the effects of CaA on the migratory capability and on the CSCs-like properties of skin cancer cells, and the molecular mechanisms underlying it are not fully understood.MethodsMalignant HaCaT cells were treated by CaA. Transwell assay was performed to determine that CaA attenuated the migratory capability; Spheroid formation assay was performed to confirm that CaA decreased the CSCs-like phenotype; Treated malignant HaCaT cells were molecularly characterized by RT-PCR, Western blots, Southwestern blot, and immunoprecipitation.ResultsIn CaA-treated malignant human keratinocyte (malignant HaCaT cells), inhibition of the migratory capability and CSCs-like phenotype were observed. CaA up-regulated the phosphorylation of p38, and down-regulated the activation of nuclear factor κB (NF-κB)/snail signal pathway. Indeed, p38 decreased the DNA-binding activity of NF-κB to the promoter of snail gene, which resulted in the transcriptional inactivation of snail. Blockage of p38 attenuated the CaA-induced inhibition of migratory capability and CSCs-like phenotype in malignant HaCaT cells.ConclusionsCaA attenuates the migratory capability and CSCs-like Properties of malignant human keratinocyte, in which, p38-mediated down-regulation of NF-κB/snail signal pathway is involved.

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“…CSCs have been identified in various human cancers, including those of the liver and lung [6,7]. Within a tumor, CSCs, which constitute a small portion of the neoplastic cells, are defined by their capacity to produce new tumors.…”

Section: Introductionmentioning

confidence: 99%

Inhibitions of epithelial to mesenchymal transition and cancer stem cells‐like properties are involved in miR‐148a‐mediated anti‐metastasis of hepatocellular carcinoma

Yan

Dong

Zhong

et al. 2013

Molecular Carcinogenesis

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The epithelial-mesenchymal transition (EMT) and acquisition of cancer stem cells (CSCs)-like properties are essential steps in the metastasis and postsurgical recurrence of hepatocellular carcinomas (HCCs). The molecular mechanisms involved, however, remain obscure. As determined by an miRNA microarray analysis, there was lower expression of miR-148a in poorly differentiated HCC tissues relative to well-differentiated HCC tissues. MHCC97H and MHCC97L (HCC cells with migratory capacity) and HCC tissues with various differentiation status were selected for further investigation. The results showed that miR-148a levels inversely correlated with the differentiation status of HCC tissues. In MHCC97H and MHCC97L cells, over-expression of miR-148a blocked the EMT process, attenuated the expression of CD90 and CD44 (biomarkers for liver cancer stem cells), and inhibited their migratory capacity. Via TargetScan and microRNA.org algorithms, miR-148a was predicted to bind to the Wnt1 mRNA 3'-UTR. Wnt1 was confirmed as a target gene of miR-148a in HCC cells, and the Wnt signal pathway was determined to be involved in the miR-148a-mediated inhibition of EMT and CSCs-like properties of MHCC97H cells. Moreover, the expression of miR-148a in nonmetastatic HCC tissues was higher than that in metastatic HCC tissues. The results suggest that miR-148a inhibits the metastasis of HCCs by blocking EMT and CSCs-like properties through effects on the Wnt signaling pathway.

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Identification of Cancer Stem Cells Derived From a Canine Lung Adenocarcinoma Cell Line

Cited by 27 publications

References 22 publications

Radioresistance of cancer stem‐like cell derived from canine tumours

Radioresistance of cancer stem‐like cell derived from canine tumours

P38/NF-κB/Snail Pathway Is Involved in Caffeic Acid-Induced Inhibition of Cancer Stem Cells-Like Properties and Migratory Capacity in Malignant Human Keratinocyte

Inhibitions of epithelial to mesenchymal transition and cancer stem cells‐like properties are involved in miR‐148a‐mediated anti‐metastasis of hepatocellular carcinoma

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