Identification of C-type isolectins in the venom of the scorpionfish Scorpaena plumieri

Andrich, Filipe; Richardson, Michael; Naumann, Gustavo B.; Cordeiro, Marta N.; Santos, Adão V.; Santos, Daniel Moreira dos; Oliveira, Juliana; Lima, Maria Elena de; Figueiredo, Suely G.

doi:10.1016/j.toxicon.2015.01.004

Cited by 19 publications

(11 citation statements)

References 23 publications

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“…Interestingly, the protein appeared to oligomerize with no loss of activity in an inhibition ELISA. A group of five isolectins (SP-1-5) were found in an agglutinating fraction of S. plumieri venom [ 136 ]. One of these isolectins was sequenced and found to have homology with fish C-type lectins.…”

Section: Venom Componentsmentioning

confidence: 99%

Bioactive Components in Fish Venoms

Ziegman

Alewood

2015

Toxins

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Animal venoms are widely recognized excellent resources for the discovery of novel drug leads and physiological tools. Most are comprised of a large number of components, of which the enzymes, small peptides, and proteins are studied for their important bioactivities. However, in spite of there being over 2000 venomous fish species, piscine venoms have been relatively underrepresented in the literature thus far. Most studies have explored whole or partially fractioned venom, revealing broad pharmacology, which includes cardiovascular, neuromuscular, cytotoxic, inflammatory, and nociceptive activities. Several large proteinaceous toxins, such as stonustoxin, verrucotoxin, and Sp-CTx, have been isolated from scorpaenoid fish. These form pores in cell membranes, resulting in cell death and creating a cascade of reactions that result in many, but not all, of the physiological symptoms observed from envenomation. Additionally, Natterins, a novel family of toxins possessing kininogenase activity have been found in toadfish venom. A variety of smaller protein toxins, as well as a small number of peptides, enzymes, and non-proteinaceous molecules have also been isolated from a range of fish venoms, but most remain poorly characterized. Many other bioactive fish venom components remain to be discovered and investigated. These represent an untapped treasure of potentially useful molecules.

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Section: Venom Componentsmentioning

confidence: 99%

Bioactive Components in Fish Venoms

Ziegman

Alewood

2015

Toxins

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“…Two lectins — (i) plumieribetin, a lectin homologous to monocot mannose-binding B-type lectin and (ii) a group of five isolectins (Sp-CL 1–5) homologous to fish C-type lectins — were purified from S. plumieri venom [27, 28]. …”

Section: Bioactive Proteins Isolated From Spvmentioning

confidence: 99%

“…The amino acid sequence of RP4 revealed homology (24–32% of identity) with various fish C-type lectins. Finally, the presence of the glycan moiety galactose-β(1 → 4)-N-acetylglucosamine was also revealed in the FV structure [28]. …”

Section: Bioactive Proteins Isolated From Spvmentioning

confidence: 99%

“…The similar chemical characteristics exhibited by RP fractions (elution in RP-HPLC and MALDI-TOF) — together with the similarities found among amino acid sequences — strongly suggest that RP1-5 are C-type lectin isoforms (isolectins) [28]. …”

Section: Bioactive Proteins Isolated From Spvmentioning

confidence: 99%

“…Considerable progress has been made in relation to the biochemical and pharmacological properties of the crude venom extract [20–23] and a few bioactive molecules have been isolated and analysed [20, 24–28]. In this review, we will focus on the discussion of the chemical and physio-pharmacological properties of S. plumieri venom along with those of the bioactive molecules isolated so far.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A review on the Scorpaena plumieri fish venom and its bioactive compounds

Campos

Menezes

Malacarne

et al. 2016

J Venom Anim Toxins Incl Trop Dis

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The most poisonous fish species found along the Brazilian coast is the spotted scorpionfish Scorpaena plumieri. Though hardly ever life-threatening to humans, envenomation by S. plumieri can be quite hazardous, provoking extreme pain and imposing significant socioeconomic costs, as the victims may require days to weeks to recover from their injuries. In this review we will walk the reader through the biological features that distinguish this species as well as the current epidemiological knowledge related to the envenomation and its consequences. But above all, we will discuss the challenges involved in the biochemical characterization of the S. plumieri venom and its compounds, focusing then on the successful isolation and pharmacological analysis of some of the bioactive molecules responsible for the effects observed upon envenomation as well as on experimental models. Despite the achievement of considerable progress, much remains to be done, particularly in relation to the non-proteinaceous components of the venom. Therefore, further studies are necessary in order to provide a more complete picture of the venom’s chemical composition and physiological effects. Given that fish venoms remain considerably less studied when compared to terrestrial venoms, the exploration of their full potential opens a myriad of possibilities for the development of new drug leads and tools for elucidating the complex physiological processes.

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The piscine arsenal: an updated review of venomous fishes

Harris

2023

Rev Fish Biol Fisheries

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Identification of C-type isolectins in the venom of the scorpionfish Scorpaena plumieri

Cited by 19 publications

References 23 publications

Bioactive Components in Fish Venoms

Bioactive Components in Fish Venoms

A review on the Scorpaena plumieri fish venom and its bioactive compounds

The piscine arsenal: an updated review of venomous fishes

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